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As with previous studies on members of this genus, we found that the isolates were multi-drug resistant, and that this resistance included clinically important beta-lactams, aminoglycosides and the fluoroquinolone ciprofloxacin. Using the minION sequencing platform (Oxford Nanopore Technologies) combined with HiSeq PE150 Illumina sequencing data, the four isolates were assembled into genomes of single contigs. Analysis of the genomes revealed potential genetic factors possibly underlying some of the specific resistances observed. Metallo-beta-lactamase activity was detected in one isolate, and the same isolate contained a putative metallo-betalactamase gene resembling pedo-2. Furthermore, several genes related to multidrug efflux systems were found using the resistance database CARD. Additionally, the present study extends our knowledge on the phylogeny of this genus, adding four new genomes to the existing 50.<\/jats:p>","DOI":"10.1093\/femsec\/fiaa088","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T19:11:54Z","timestamp":1588965114000},"source":"Crossref","is-referenced-by-count":16,"title":["Whole genome sequencing and antibiotic diffusion assays, provide new insight on drug resistance in the genus Pedobacter"],"prefix":"10.1093","volume":"96","author":[{"given":"Ingvild Falkum","family":"Ullmann","sequence":"first","affiliation":[{"name":"Oslo Metropolitan University, Faculty of Health Sciences, Department of Life Sciences and Health, NO-0130, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders Benteson","family":"Nygaard","sequence":"first","affiliation":[{"name":"Oslo Metropolitan University, Faculty of Health Sciences, Department of Life Sciences and Health, NO-0130, Oslo, 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