{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:31:39Z","timestamp":1790418699641,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T00:00:00Z","timestamp":1596585600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bacillus sp. Abq, belonging to Bacillus cereus sensu lato, was isolated from an aquifer in New Mexico, USA and phylogenetically classified. The isolate possesses the unusual property of precipitating Pb(II) by using cysteine, which is degraded intracellularly to hydrogen sulfide (H2S). H2S is then exported to the extracellular environment to react with Pb(II), yielding PbS (galena). Biochemical and growth tests showed that other sulfur sources tested (sulfate, thiosulfate, and methionine) were not reduced to hydrogen sulfide. Using equimolar concentration of cysteine, 1\u00a0mM of soluble Pb(II) was removed from Lysogeny Broth (LB) medium within 120\u00a0h of aerobic incubation forming black, solid PbS, with a removal rate of 2.03\u00a0\u00b5g L\u22121 h\u22121 (\u223c8.7 \u00b5M L\u22121 h\u22121). The mineralogy of biogenic PbS was characterized and confirmed by XRD, HRTEM, and EDX. Electron microscopy and electron diffraction identified crystalline PbS nanoparticles with a diameter &amp;lt;10\u00a0nm, \u00a0localized in the extracellular matrix and on the surface of the cells. This is the first study demonstrating the use of cysteine in Pb(II) precipitation as insoluble PbS and it may pave the way to PbS recovery from secondary resources, such as Pb-laden industrial effluents.<\/jats:p>","DOI":"10.1093\/femsec\/fiaa151","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T19:29:29Z","timestamp":1596569369000},"source":"Crossref","is-referenced-by-count":17,"title":["PbS biomineralization using cysteine: Bacillus cereus and the sulfur rush"],"prefix":"10.1093","author":[{"given":"Lucian C","family":"Staicu","sequence":"first","affiliation":[{"name":"Faculty of Biology, University of Warsaw, Miecznikowa 1, 02\u2013096 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina J","family":"Wojtowicz","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Warsaw, Miecznikowa 1, 02\u2013096 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mih\u00e1ly","family":"P\u00f3sfai","sequence":"additional","affiliation":[{"name":"Department of Earth and Environmental Sciences, University of Pannonia, Egyetem u. 10, H-8200, Veszpr\u00e9m, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter","family":"Pekker","sequence":"additional","affiliation":[{"name":"Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem u. 10, H-8200, Veszpr\u00e9m, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Gorecki","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Warsaw, Miecznikowa 1, 02\u2013096 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona L","family":"Jordan","sequence":"additional","affiliation":[{"name":"Department of Biology, University of New Mexico, MSCO3 2020, Albuquerque NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry L","family":"Barton","sequence":"additional","affiliation":[{"name":"Department of Biology, University of New Mexico, MSCO3 2020, Albuquerque NM 87131, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,8,5]]},"container-title":["FEMS Microbiology Ecology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femsec\/advance-article-pdf\/doi\/10.1093\/femsec\/fiaa151\/33569528\/fiaa151.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsec\/advance-article-pdf\/doi\/10.1093\/femsec\/fiaa151\/33569528\/fiaa151.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T16:21:02Z","timestamp":1596644462000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsec\/advance-article\/doi\/10.1093\/femsec\/fiaa151\/5881300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/femsec\/fiaa151","relation":{},"ISSN":["0168-6496","1574-6941"],"issn-type":[{"value":"0168-6496","type":"print"},{"value":"1574-6941","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,5]]},"article-number":"fiaa151"}}