{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:46:32Z","timestamp":1791474392651,"version":"4.3.4"},"reference-count":89,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T00:00:00Z","timestamp":1620777600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014423","name":"Environmental Restoration and Conservation Agency of Japan","doi-asserted-by":"publisher","award":["5-1701"],"award-info":[{"award-number":["5-1701"]}],"id":[{"id":"10.13039\/100014423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,21]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Chloroethenes are common soil and groundwater pollutants. Their dechlorination is impacted by environmental factors, such as the presence of metal ions. We here investigated the effect of ferrous iron on bacterial reductive dechlorination of chloroethenes and on methanogen community. Reductive dechlorination of tetrachloroethene was assayed with a groundwater sample originally containing 6.3\u00a0\u00d7 103 copies mL\u22121 of Dehalococcoides 16S rRNA gene and 2\u00a0mg\u2009L\u22121 of iron. Supplementation with 28\u00a0mg\u2009L\u22121 of ferrous iron enhanced the reductive dechlorination of cis-dichloroethene (cis-DCE) and vinyl chloride in the presence of methanogens. The supplementation shortened the time required for complete dechlorination of 1\u00a0mg\u2009L\u22121 of tetrachloroethene to ethene and ethane from 84 to 49 d. Methanogens, such as Candidatus \u2018Methanogranum\u2019, Methanomethylovorans and Methanocorpusculum, were significantly more abundant in iron-supplemented cultures than in non-supplemented cultures (P\u00a0&amp;lt;\u00a00.01). Upon methanogen growth inhibition by 2-bromoethanesulfonate and in the absence of iron supplementation, cis-DCE was not dechlorinated. Further, iron supplementation induced 71.3% dechlorination of cis-DCE accompanied by an increase in Dehalococcoides 16S rRNA and dehalogenase vcrA gene copies but not dehalogenase tceA gene copies. These observations highlight the cooperative effect of iron and methanogens on the reductive dechlorination of chloroethenes by Dehalococcoides spp.<\/jats:p>","DOI":"10.1093\/femsec\/fiab069","type":"journal-article","created":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T13:11:24Z","timestamp":1620652284000},"source":"Crossref","is-referenced-by-count":11,"title":["Effects of ferrous iron supplementation on reductive dechlorination of tetrachloroethene and on methanogenic microbial community"],"prefix":"10.1093","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9797-3674","authenticated-orcid":false,"given":"Miho","family":"Yoshikawa","sequence":"first","affiliation":[{"name":"Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki 305-8567, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki 305-8567, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshishige","family":"Kawabe","sequence":"additional","affiliation":[{"name":"Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki 305-8567, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiki","family":"Katayama","sequence":"additional","affiliation":[{"name":"Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki 305-8567, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,5,12]]},"reference":[{"key":"2021070721353727800_bib1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0168-6445(03)00055-X","article-title":"Bacterial iron homeostasis","volume":"27","author":"Andrews","year":"2003","journal-title":"FEMS Microbiol Rev"},{"key":"2021070721353727800_bib2","doi-asserted-by":"crossref","first-page":"109282","DOI":"10.1016\/j.rser.2019.109282","article-title":"A review of the effects of iron compounds on methanogenesis in anaerobic environments","volume":"113","author":"Baek","year":"2019","journal-title":"Renewable Sustainable Energy Rev"},{"key":"2021070721353727800_bib3","doi-asserted-by":"crossref","first-page":"103480","DOI":"10.1016\/j.jconhyd.2019.04.003","article-title":"Contributions of biotic and abiotic pathways to anaerobic trichloroethene transformation in low permeability source 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