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Here, we aimed to develop a plant model to study C. gattii\u2013plant interaction, since it is unclear how it affects the plant and the yeast. We tested three inoculation methods (scarification, infiltration, and abrasion) in three plant species: Arabidopsis thaliana, Nicotiana tabacum, and N. benthamiana. Cryptococcus gattii was able to grow in all three models, with a peak of yeast cell burden after 7 days, without any pathological effects. Furthermore, the fungal burden was reduced later, confirming that C. gattii is not a phytopathogen. Cryptococcus gattii proliferation was higher in N. benthamiana, which presented an increase in the hydrogen peroxide content, antioxidant system activity, and indoleacetic acid (IAA) production. Cryptococcus gattii colonies recovered from N. benthamiana presented lower ergosterol content, reduced capsule, and increased growth rate in vitro and inside macrophages. In vitro, IAA altered C. gattii morphology and susceptibility to antifungal drugs. We hypothesize that C. gattii can temporarily colonize plant living tissues, which can be a potential reservoir of yeast virulence, with further dissemination to the environment, birds, and mammals. In conclusion, N. benthamiana is suitable for studying C. gattii\u2013plant interaction.<\/jats:p>","DOI":"10.1093\/femsec\/fiac036","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T21:52:48Z","timestamp":1648158768000},"source":"Crossref","is-referenced-by-count":5,"title":["<i>Nicotiana benthamiana<\/i> as a model for studying <i>Cryptococcus<\/i>\u2013plant interaction"],"prefix":"10.1093","volume":"98","author":[{"given":"Jo\u00e3o C M","family":"Dornelas","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos, 6627, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marliete C","family":"Costa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia, Departamento de Microbiologia, Instituto de 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Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gracielle P P","family":"Bragan\u00e7a","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e2nica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos, 6627, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosy M S","family":"Isaias","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e2nica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos, 6627, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00falio C M","family":"Brito","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Ezequiel Dias (FUNED), Rua Conde Pereira Carneiro, 80, Gameleira, CEP 30.510-000, Belo Horizonte, MG, 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