{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T01:14:44Z","timestamp":1791508484457,"version":"4.3.4"},"reference-count":144,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,13]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bioaugmentation is a promising strategy for enhancing trichloroethylene (TCE) degradation in fractured rock. However, slow or incomplete biodegradation can lead to stalling at degradation byproducts such as 1,2-dichloroethene (cis-DCE) and vinyl chloride (VC). Over the course of 7 years, we examined the response of groundwater microbial populations in a bioaugmentation test where an emulsified vegetable oil solution (EOS\u00ae) and a dechlorinating consortium (KB-1\u00ae), containing the established dechlorinator Dehalococcoides (DHC), were injected into a TCE-contaminated fractured rock aquifer. Indigenous microbial communities responded within 2 days to added substrate and outcompeted KB-1\u00ae, and over the years of monitoring, several other notable turnover events were observed. Concentrations of ethene, the end product in reductive dechlorination, had the strongest correlations (P&amp;lt; .05) with members of Candidatus Colwellbacteria but their involvement in reductive dechlorination is unknown and warrants further investigation.DHC never exceeded 0.6% relative abundance of groundwater microbial communities, despite its previously presumed importance at the site. Increased concentrations of carbon dioxide, acetic acid, and methane were positively correlated with increasing ethene concentrations; however, concentrations of cis-DCE and VC remained high by the end of the monitoring period suggesting preferential enrichment of indigenous partial dechlorinators over bioaugmented complete dechlorinators. This study highlights the importance of characterizing in situ microbial populations to understand how they can potentially enhance or inhibit augmented TCE degradation.<\/jats:p>","DOI":"10.1093\/femsec\/fiac077","type":"journal-article","created":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T18:04:45Z","timestamp":1656093885000},"source":"Crossref","is-referenced-by-count":19,"title":["Microbial community response to a bioaugmentation test to degrade trichloroethylene in a fractured rock aquifer, Trenton, N.J"],"prefix":"10.1093","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2702-0410","authenticated-orcid":false,"given":"Jennifer C","family":"Underwood","sequence":"first","affiliation":[{"name":"U.S. Geological Survey, Water Mission Area , Boulder, CO 80303, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1534-3025","authenticated-orcid":false,"given":"Denise M","family":"Akob","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, Geology, Energy and Minerals Science Center , 12201 Sunrise Valley Drive, Mailstop 954 , Reston, VA 20192, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9236-587X","authenticated-orcid":false,"given":"Michelle M","family":"Lorah","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, MD-DE-DC Water Science Center , 5522 Research Park Drive , Baltimore, MD 21228, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1716-4768","authenticated-orcid":false,"given":"Thomas E","family":"Imbrigiotta","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, New Jersey Water Science Center , 3450 Princeton Pike , Suite 110, Lawrenceville, NJ 08648, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2791-8503","authenticated-orcid":false,"given":"Ronald W","family":"Harvey","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, Water Mission Area , Boulder, CO 80303, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0128-3685","authenticated-orcid":false,"given":"Claire R","family":"Tiedeman","sequence":"additional","affiliation":[{"name":"U.S. Geological Survey, Water Mission Area , Menlo Park, CA 94025, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,24]]},"reference":[{"key":"2022101911291198500_bib1","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1016\/j.watres.2008.03.015","article-title":"Microbial activity and distribution during enhanced contaminant dissolution from a NAPL source 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