{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T06:45:43Z","timestamp":1772174743601,"version":"3.50.1"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T00:00:00Z","timestamp":1662422400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001332","name":"CSIR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001332","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Herein, Bacillus subtilis PBE-8\u2032s biocontrol efficacy was evaluated through physiological and metabolic approaches against Fusarium oxysporum f.sp. lycopersici (FOL). The study elaborates on PBE-8\u2032s cell-free filtrate (CFF) antifungal activity through mycelial growth inhibition, metabolite profiling, and substrates utilization patterns. Additionally, under different CFF concentrations, reduction in spore count (94%\u201355%), biomass (50%), and cytoplasmic bulbous protrusions in mycelia were also observed. Furthermore, the effect of bacterial CFF on FOL metabolism was confirmed through GC-MS. CFF suppresses the concentration of aliphatic amino acids like L-valine, L-leucine, L-Isoleucine, glycine, and fatty acids such as linoleic acid and \u03b1- linolenic acid during the co-culturing conditions, which are essential for pathogenicity and resistance against host's systemic acquired resistance. The phenotype microarray assay revealed that CFF-treated FOL shows phenotype loss in 507 (56.58%) out of 896 substrates. Among 507, twenty-seven substrates showed significant phenotype loss, among which four substrates such as L-glutamic acid, L-glutamine, ammonia, and L-arginine are common in different crucial metabolic pathways of FOL, like alanine, aspartate, and glutamate metabolism, arginine and proline, carbon metabolism, arginine biosynthesis, nitrogen metabolism, amino-acyl tRNA synthesis, and biosynthesis of amino acids. The results suggest that PBE-8 CFF has certain antifungal metabolites that hinder the fungal metabolic pathways.<\/jats:p>","DOI":"10.1093\/femsec\/fiac102","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T15:42:51Z","timestamp":1662478971000},"source":"Crossref","is-referenced-by-count":10,"title":["Phenotype microarray analysis reveals the biotransformation of <i>Fusarium oxysporum f.sp. lycopersici<\/i> influenced by <i>Bacillus subtilis<\/i> PBE-8 metabolites"],"prefix":"10.1093","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3288-2736","authenticated-orcid":false,"given":"Aradhana","family":"Mishra","sequence":"first","affiliation":[{"name":"Division of Microbial Technology, CSIR-National Botanical Research Institute , Lucknow, UP 226001 , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad, 201002, UP , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arpita","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Division of Microbial Technology, CSIR-National Botanical Research Institute , Lucknow, UP 226001 , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad, 201002, UP , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyanka","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Division of Microbial Technology, CSIR-National Botanical Research Institute , Lucknow, UP 226001 , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad, 201002, UP , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shipra","family":"Pandey","sequence":"additional","affiliation":[{"name":"Division of Microbial Technology, CSIR-National Botanical Research Institute , Lucknow, UP 226001 , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad, 201002, UP , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashish","family":"Dwivedi","sequence":"additional","affiliation":[{"name":"Photobiology Division, CSIR-Indian Institute of Toxicology Research , Lucknow, UP 226001 , India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,9,6]]},"reference":[{"key":"2022100313200574400_bib1","first-page":"5","article-title":"Efficacy of cell-free supernatant from Bacillus subtilis ET-1, an iturin a producer strain, on biocontrol of green and gray mold","volume-title":"Postharvest Biol Technol","author":"Ambrico","year":"2017"},{"key":"2022100313200574400_bib2","first-page":"151","article-title":"Global nutrient profiling by phenotype microarrays: a tool complementing genomic and proteomic studies in conidial fungi","volume-title":"J Zhejiang Univ Sci B","author":"Atanasova","year":"2010"},{"key":"2022100313200574400_bib3","first-page":"2435","article-title":"The effect of Fusarium verticillioides fumonisins on fatty acids, sphingolipids, and oxylipins in maize germlings","volume-title":"Int J Mol Sci","author":"Beccaccioli","year":"2021"},{"key":"2022100313200574400_bib4","first-page":"165","article-title":"Masked mycotoxins: a review","volume-title":"Mol Nutr Food Res","author":"Berthiller","year":"2013"},{"key":"2022100313200574400_bib5","first-page":"105","article-title":"Metabolic profiling for studying chemotype variations in Withania somnifera (L.) 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