{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:48:36Z","timestamp":1790887716823,"version":"4.1.0"},"reference-count":98,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T00:00:00Z","timestamp":1673308800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EAR-1833525"],"award-info":[{"award-number":["EAR-1833525"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sideroxydans sp. CL21 is a microaerobic, acid-tolerant Fe(II)-oxidizer, isolated from the Schl\u00f6ppnerbrunnen fen. Since the genome size of Sideroxydans sp. CL21 is 21% larger than that of the neutrophilic Sideroxydans lithotrophicus ES-1, we hypothesized that strain CL21 contains additional metabolic traits to thrive in the fen. The common genomic content of both strains contains homologs of the putative Fe(II) oxidation genes, mtoAB and cyc2. A large part of the accessory genome in strain CL21 contains genes linked to utilization of alternative electron donors, including NiFe uptake hydrogenases, and genes encoding lactate uptake and utilization proteins, motility and biofilm formation, transposable elements, and pH homeostasis mechanisms. Next, we incubated the strain in different combinations of electron donors and characterized the fen microbial communities. Sideroxydans spp. comprised 3.33% and 3.94% of the total relative abundance in the peatland soil and peatland water, respectively. Incubation results indicate Sideroxydans sp. CL21 uses H2 and thiosulfate, while lactate only enhances growth when combined with Fe, H2, or thiosulfate. Rates of H2 utilization were highest in combination with other substrates. Thus, Sideroxydans sp. CL21 is a mixotroph, growing best by simultaneously using substrate combinations, which helps to thrive in dynamic and complex habitats.<\/jats:p>","DOI":"10.1093\/femsec\/fiac156","type":"journal-article","created":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T12:30:40Z","timestamp":1673008240000},"source":"Crossref","is-referenced-by-count":12,"title":["Mixotrophy broadens the ecological niche range of the iron oxidizer\n                    <i>Sideroxydans<\/i>\n                    sp. CL21 isolated from an iron-rich peatland"],"prefix":"10.1093","volume":"99","author":[{"given":"Rebecca E","family":"Cooper","sequence":"first","affiliation":[{"name":"Aquatic Geomicrobiology , , 07743 Jena , Germany"},{"name":"Institute of Biodiversity, Friedrich Schiller University Jena , , 07743 Jena , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Finck","sequence":"additional","affiliation":[{"name":"Aquatic Geomicrobiology , , 07743 Jena , Germany"},{"name":"Institute of Biodiversity, Friedrich Schiller University Jena , , 07743 Jena , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Chan","sequence":"additional","affiliation":[{"name":"School of Marine Science and Policy, University of Delaware , Newark, DE 19716 , United States"},{"name":"Delaware Biotechnology Institute, University of Delaware , Newark, DE 19713 , United States"},{"name":"Department of Earth Sciences, University of Delaware , Newark, DE 19716 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5396-0975","authenticated-orcid":false,"given":"Kirsten","family":"K\u00fcsel","sequence":"additional","affiliation":[{"name":"Aquatic Geomicrobiology , , 07743 Jena , Germany"},{"name":"Institute of Biodiversity, Friedrich Schiller University Jena , , 07743 Jena , Germany"},{"name":"German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig , 04103 Leipzig , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,1,9]]},"reference":[{"key":"2023021402015660200_bib1","doi-asserted-by":"crossref","first-page":"7609","DOI":"10.1021\/es061018y","article-title":"Characterizing the redox status in three different forested wetlands with geochemical data","volume":"40","author":"Alewell","year":"2006","journal-title":"Environ Sci Technol"},{"key":"2023021402015660200_bib2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3354\/ame01753","article-title":"Minor revisin to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton","volume":"75","author":"Apprill","year":"2015","journal-title":"Aquat Microb Ecol"},{"key":"2023021402015660200_bib3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST Server: rapid annotations using subsystems technology","volume":"9","author":"Aziz","year":"2008","journal-title":"BMC Genomics"},{"key":"2023021402015660200_bib4","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1093\/jxb\/erm297","article-title":"Multiple Rubisco forms in proteobacteria: their functional significance in relation to CO2 acquisition by the CBB cycle","volume":"59","author":"Badger","year":"2008","journal-title":"J Exp Bot"},{"key":"2023021402015660200_bib5","doi-asserted-by":"crossref","first-page":"332","DOI":"10.3389\/fmicb.2015.00332","article-title":"Characterization of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth","volume":"6","author":"Beckwith","year":"2015","journal-title":"Frontiers in Microbiology"},{"key":"2023021402015660200_bib6","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1093\/gbe\/evv148","article-title":"A model of genome size evolution for prokaryotes in stable and fluctuating environments","volume":"7","author":"Bentkowski","year":"2015","journal-title":"Genome Biol Evolut"},{"key":"2023021402015660200_bib7","doi-asserted-by":"crossref","DOI":"10.1093\/femsec\/fiaa036","article-title":"Genome reconstruction reveals distinct assemblages of Gallionellaceae in surface and subsurface redox transition zones","volume":"96","author":"Bethencourt","year":"2020","journal-title":"FEMS Microbiol Ecol"},{"key":"2023021402015660200_bib8","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1127\/archiv-hydrobiol\/154\/2002\/561","article-title":"Iron, sulfur, and dissolved carbon dynamics in a northern peatland","volume":"154","author":"Blodau","year":"2002","journal-title":"Fundam Appl Limnol"},{"key":"2023021402015660200_bib9","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/978-3-540-33187-2_10","article-title":"Ecological aspects of microbes and microbial communities inhabiting the rhizosphere of wetland plants","volume-title":"Wetlands and Natural Resource Management","author":"Bodelier","year":"2006"},{"key":"2023021402015660200_bib10","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/nclimate1781","article-title":"Biogeochemical plant-soil microbe feedback in response to climate warming in peatlands","volume":"3","author":"Bragazza","year":"2013","journal-title":"Nature Climate Change"},{"key":"2023021402015660200_bib11","doi-asserted-by":"crossref","first-page":"8365","DOI":"10.1038\/srep08365","article-title":"RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes","volume":"5","author":"Brettin","year":"2015","journal-title":"Sci Rep"},{"key":"2023021402015660200_bib12","doi-asserted-by":"crossref","first-page":"3069","DOI":"10.1021\/es048613p","article-title":"Simultaneous utilization of acetate and hydrogen by Geobacter sulfurreducens and implications for use of hydrogen as an indicator of redox conditions","volume":"39","author":"Brown","year":"2005","journal-title":"Environ Sci Technol"},{"key":"2023021402015660200_bib13","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.1111\/1462-2920.14328","article-title":"Microbial anaerobic Fe(II) oxidation - Ecology, mechanisms and environmental implications","volume":"20","author":"Bryce","year":"2018","journal-title":"Environ Microbiol"},{"key":"2023021402015660200_bib14","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1073\/pnas.1000080107","article-title":"Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample","volume":"108","author":"Caporaso","year":"2011","journal-title":"Proc Natl Acad Sci"},{"key":"2023021402015660200_bib15","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1038\/ismej.2012.8","article-title":"Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms","volume":"6","author":"Caporaso","year":"2012","journal-title":"ISME J"},{"key":"2023021402015660200_bib16","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1038\/ismej.2017.112","article-title":"Mixotrophy drives niche expansion of verrucomicrobial methanotrophs","volume":"11","author":"Carere","year":"2017","journal-title":"ISME J"},{"key":"2023021402015660200_bib17","doi-asserted-by":"crossref","first-page":"796","DOI":"10.3389\/fmicb.2016.00796","article-title":"The architecture of iron microbial mats reflects the adaptation of chemolithotrophic iron oxidation in freshwater and marine environments","volume":"7","author":"Chan","year":"2016","journal-title":"Frontiers in Microbiology"},{"key":"2023021402015660200_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11274-019-2617-x","article-title":"Performance and microbial community pro-files in pilot-scale biofilter for ammonia, iron and manganese removal at different dissolved oxygen concentrations","volume":"35","author":"Cheng","year":"2019","journal-title":"World J Microbiol Biotechnol"},{"key":"2023021402015660200_bib19","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1186\/s12864-017-3888-y","article-title":"Relating genomic characteristics to environmental preferences and ubiquity in different microbial taxa","volume":"18","author":"Cobo-Simon","year":"2017","journal-title":"BMC Genomics"},{"key":"2023021402015660200_bib20","doi-asserted-by":"crossref","first-page":"e01444","DOI":"10.1128\/MRA.01444-19","article-title":"Draft genome sequence of Sideroxydans sp. strain CL21, an Fe(II)-oxidizing bacterium","volume":"9","author":"Cooper","year":"2020","journal-title":"Microbiol Resour Announc"},{"key":"2023021402015660200_bib21","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.1038\/s41396-020-0718-z","article-title":"Iron is not everything: unexpected complex metabolic responses between iron-cycling microorganisms","volume":"14","author":"Cooper","year":"2020","journal-title":"ISME J"},{"key":"2023021402015660200_bib22","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1128\/JB.186.10.3022-3028.2004","article-title":"Identification of an uptake hydrogenase required for hydrogen-dependent reduction of Fe(III) and other electron acceptors by Geobacter sulfurreducens","volume":"186","author":"Coppi","year":"2004","journal-title":"J Bacteriol"},{"key":"2023021402015660200_bib23","first-page":"e4320","article-title":"Linking pangenomes and metagenomes: the prochlorococcus metapangenome","volume-title":"Peer J","author":"Delmont","year":"2018"},{"key":"2023021402015660200_bib24","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1126\/science.1120250","article-title":"Community genomics among stratified microbial assemblages in the ocean's interior","volume":"311","author":"DeLong","year":"2006","journal-title":"Science"},{"key":"2023021402015660200_bib25","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1093\/bioinformatics\/btq234","article-title":"Characterization of the N-ATPase, a distinct, laterally transferred Na+-translocating form of the bacterial F-type membrane atpase","volume":"26","author":"Dibrova","year":"2010","journal-title":"Bioinformatics"},{"key":"2023021402015660200_bib26","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1758-2229.2009.00050.x","article-title":"Intermediary ecosystem metabolism as a main driver of methanogenesis in acidic wetland soil","volume":"1","author":"Drake","year":"2009","journal-title":"Environ Microbiol Rep"},{"key":"2023021402015660200_bib27","doi-asserted-by":"crossref","first-page":"3358","DOI":"10.1016\/j.gca.2008.04.035","article-title":"Low-oxygen and chemical kinetic constraints on the geochemical niche of neutrophilic iron(II) oxidizing microorganisms","volume":"72","author":"Druschel","year":"2008","journal-title":"Geochim Cosmochim Acta"},{"key":"2023021402015660200_bib28","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"2023021402015660200_bib29","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1080\/01490451.2019.1686667","article-title":"Growth of iron-Oxidizing bacteria Gallionella ferruginea and Leptothrix cholodnii in oligotrophic environments: Ca, Mg, and C as limiting factors and G. ferruginea necromass as C-Source","volume":"37","author":"Eggerichs","year":"2020","journal-title":"Geomicrobiol J"},{"key":"2023021402015660200_bib30","doi-asserted-by":"crossref","first-page":"4784","DOI":"10.1128\/aem.63.12.4784-4792.1997","article-title":"Isolation and characterization of novel iron-oxidizing bacteria that grow at circumneutral pH","volume":"63","author":"Emerson","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"2023021402015660200_bib31","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0076-6879(05)97006-7","article-title":"Enrichment and isolation of iron-oxidizing bacteria at neutral pH","volume":"397","author":"Emerson","year":"2005","journal-title":"Methods Enzymol"},{"key":"2023021402015660200_bib32","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1146\/annurev.micro.112408.134208","article-title":"Iron-oxidizing bacteria: an environmental and genomic perspective","volume":"64","author":"Emerson","year":"2010","journal-title":"Annu Rev Microbiol"},{"key":"2023021402015660200_bib33","doi-asserted-by":"crossref","first-page":"254","DOI":"10.3389\/fmicb.2013.00254","article-title":"Comparative genomics of freshwater Fe-oxidizing bacteria: implications for physiology, ecology, and systematics","volume":"4","author":"Emerson","year":"2013","journal-title":"Frontiers in Microbiology"},{"key":"2023021402015660200_bib34","first-page":"153","article-title":"Chapter 9 the Kdp system: a bacterial K+ transport atpase","volume-title":"Current Topics in Membranes and Transport","author":"Epstein","year":"1985"},{"key":"2023021402015660200_bib35","doi-asserted-by":"crossref","first-page":"e1319","DOI":"10.7717\/peerj.1319","article-title":"Anvi'o: an advanced analysis and visualization platform for 'omics data","volume":"3","author":"Eren","year":"2015","journal-title":"PeerJ"},{"key":"2023021402015660200_bib36","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/s41564-020-00834-3","article-title":"Community-led, integrated, reproducible multi-omics with anvi'o","volume":"6","author":"Eren","year":"2021","journal-title":"Nature Microbiology"},{"key":"2023021402015660200_bib37","doi-asserted-by":"crossref","first-page":"390","DOI":"10.3389\/fmicb.2013.00390","article-title":"Surprising abundance of Gallionella-related iron oxidizers in creek sediments at pH 4.4 or at high heavy metal concentrations","volume":"4","author":"Fabisch","year":"2013","journal-title":"Frontiers in Microbiology"},{"key":"2023021402015660200_bib38","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/ismej.2013.197","article-title":"Ecological succession among iron-oxidizing bacteria","volume":"8","author":"Fleming","year":"2014","journal-title":"ISME J"},{"key":"2023021402015660200_bib39","doi-asserted-by":"crossref","first-page":"37","DOI":"10.3389\/fmicb.2020.00037","article-title":"FeGenie: a comprehensive tool for the identification of iron genes and iron gene neighborhoods in genome and metagenome assemblies","volume":"11","author":"Garber","year":"2020","journal-title":"Frontiers in Microbiology"},{"key":"2023021402015660200_bib40","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1038\/s41396-018-0091-3","article-title":"Potential for primary productivity in a globally-distributed bacterial phototroph","volume":"12","author":"Graham","year":"2018","journal-title":"ISME J"},{"key":"2023021402015660200_bib41","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/j.1574-6941.2012.01352.x","article-title":"Origin and fate of acetate in an acidic fen","volume":"81","author":"H\u00e4drich","year":"2012","journal-title":"FEMS Microbiol Ecol"},{"key":"2023021402015660200_bib42","doi-asserted-by":"crossref","DOI":"10.1093\/femsec\/fiz034","article-title":"Microbial Fe(II) oxidation by Sideroxydans lithotrophicus ES-1 in the presence of Schl\u00f6ppnerbrunnen fen-derived humic acids","volume":"95","author":"H\u00e4drich","year":"2019","journal-title":"FEMS Microbiol Ecol"},{"key":"2023021402015660200_bib43","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.1128\/AEM.00193-08","article-title":"Anaerobic consumers of monosaccharides in a moderately acidic fen","volume":"74","author":"Hamberger","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"2023021402015660200_bib44","first-page":"5339","article-title":"O2-Tolerant H2 activation by an isolated large subunit of a [NiFe] hydrogenase","volume-title":"Biochemistry","author":"Hartmann","year":"2018"},{"key":"2023021402015660200_bib45","doi-asserted-by":"crossref","first-page":"2520","DOI":"10.1128\/JB.186.9.2520-2522.2004","article-title":"Metabolic versatility of prokaryotes for urea decomposition","volume":"186","author":"Hausinger","year":"2004","journal-title":"J Bacteriol"},{"key":"2023021402015660200_bib46","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.1038\/ismej.2016.42","article-title":"Consortia of low-abundance bacteria drive sulfate reduction-dependent degradation of fermentation products in peat soil microcosms","volume":"10","author":"Hausmann","year":"2016","journal-title":"ISME J"},{"key":"2023021402015660200_bib47","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1038\/s41396-018-0077-1","article-title":"Peatland acidobacteria with a dissimilatory sulfur metabolism","volume":"12","author":"Hausmann","year":"2018","journal-title":"ISME J"},{"key":"2023021402015660200_bib48","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.3389\/fmicb.2017.01584","article-title":"Comparative genomic analysis of neutrophilic iron(II) oxidizer genomes for candidate genes in extracellular electron transfer","volume":"8","author":"He","year":"2017","journal-title":"Frontiers in Microbiology"},{"key":"2023021402015660200_bib49","doi-asserted-by":"crossref","first-page":"126306","DOI":"10.1016\/j.syapm.2022.126306","article-title":"Candidatus ferrigenium straubiae' sp. nov., \u2018Candidatus ferrigenium bremense\u2019 sp. nov., \u2018Candidatus ferrigenium altingense\u2019 sp. nov., are autotrophic Fe(II)-oxidizing bacteria of the family Gallionellaceae","volume":"45","author":"Huang","year":"2022","journal-title":"Syst Appl Microbiol"},{"key":"2023021402015660200_bib50","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.bbabio.2012.10.001","article-title":"Insight into the evolution of the iron oxidation pathways","volume":"1827","author":"Ilbert","year":"2013","journal-title":"Biochimica Et Biophysica Acta (BBA) - Bioenergetics"},{"key":"2023021402015660200_bib51","doi-asserted-by":"crossref","first-page":"96","DOI":"10.3389\/fmicb.2012.00096","article-title":"Redox transformations of iron at extremely low pH: fundamental and applied aspects","volume":"3","author":"Johnson","year":"2012","journal-title":"Front Microbiol"},{"key":"2023021402015660200_bib52","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.femsle.2005.02.023","article-title":"Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP-dependent degradation pathway specific to urea","volume":"245","author":"Kanamori","year":"2005","journal-title":"FEMS Microbiol Lett"},{"key":"2023021402015660200_bib53","doi-asserted-by":"crossref","first-page":"e0107421","DOI":"10.1128\/mBio.01074-21","article-title":"Iron oxidation by a fused cytochrome-porin common to diverse iron-oxidizing bacteria","volume":"12","author":"Keffer","year":"2021","journal-title":"Mbio"},{"key":"2023021402015660200_bib54","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/nar\/gks808","article-title":"Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies","volume":"41","author":"Klindworth","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2023021402015660200_bib55","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.soilbio.2009.02.030","article-title":"Impact of experimental drought and rewetting on redox transformations and methanogenesis in mesocosms of a northern fen soil","volume":"41","author":"Knorr","year":"2009","journal-title":"Soil Biol Biochem"},{"key":"2023021402015660200_bib56","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.geoderma.2009.08.023","article-title":"Dynamics of redox processes in a minerotrophic fen exposed to a water table manipulation","volume":"153","author":"Knorr","year":"2009","journal-title":"Geoderma"},{"key":"2023021402015660200_bib57","doi-asserted-by":"crossref","first-page":"3160","DOI":"10.1073\/pnas.0308653100","article-title":"Trends between gene content and genome size in prokaryotic species with larger genomes","volume":"101","author":"Konstantinidis","year":"2004","journal-title":"Proc Natl Acad Sci"},{"key":"2023021402015660200_bib58","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/s00203-001-0396-9","article-title":"Evaluation of non-cyanobacterial genome sequences for occurrence of genes encoding proteins homologous to cyanophycin synthetase and cloning of an active cyanophycin synthetase from Acinetobacter sp. strain DSM 587","volume":"177","author":"Krehenbrink","year":"2002","journal-title":"Arch Microbiol"},{"key":"2023021402015660200_bib59","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1016\/j.scitotenv.2018.07.258","article-title":"Iron-organic matter complexes accelerate microbial iron cycling in an iron-rich fen","volume":"646","author":"K\u00fcgler","year":"2019","journal-title":"Sci Total Environ"},{"key":"2023021402015660200_bib60","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s10534-020-00258-w","article-title":"Rhizobactin B is the preferred siderophore by a novel Pseudomonas isolate to obtain iron from dissolved organic matter in peatlands","volume":"33","author":"K\u00fcgler","year":"2020","journal-title":"Biometals"},{"key":"2023021402015660200_bib61","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.5194\/bg-5-1537-2008","article-title":"Microbial reduction of iron and porewater biogeochemistry in acidic peatlands","volume":"5","author":"K\u00fcsel","year":"2008","journal-title":"Biogeosciences"},{"key":"2023021402015660200_bib62","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1073\/pnas.75.7.3216","article-title":"Identification of the structural proteins of an ATP-driven potassium transport system in Escherichia coli","volume":"75","author":"Laimins","year":"1978","journal-title":"Proc Natl Acad Sci"},{"key":"2023021402015660200_bib63","first-page":"221","article-title":"Importance of allochthonous and autochthonous dissolved organic matter in Fe(II) oxidation: a case study in Shizugawa Bay watershed, Japan","volume-title":"Chemosphere","author":"Lee","year":"2017"},{"key":"2023021402015660200_bib64","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.1128\/JB.01652-14","article-title":"The terminal oxidase cbb3 functions in redox control of magnetite biomineralization in Magnetospirillum gryphiswaldense","volume":"196","author":"Li","year":"2014","journal-title":"J Bacteriol"},{"key":"2023021402015660200_bib65","doi-asserted-by":"crossref","first-page":"37","DOI":"10.3389\/fmicb.2012.00037","article-title":"Identification and characterization of MtoA: a decaheme c-type cytochrome of the neutrophilic Fe(II)-oxidizing bacterium Sideroxydans lithotrophicus ES-1","volume":"3","author":"Liu","year":"2012","journal-title":"Frontiers in Microbiology"},{"key":"2023021402015660200_bib66","doi-asserted-by":"crossref","first-page":"6998","DOI":"10.1128\/AEM.70.12.6998-7009.2004","article-title":"Microarray and functional gene analyses of sulfate-reducing prokaryotes in low-sulfate, acidic fens reveal cooccurrence of recognized genera and novel lineages","volume":"70","author":"Loy","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"2023021402015660200_bib67","doi-asserted-by":"crossref","first-page":"8174","DOI":"10.1128\/AEM.01931-10","article-title":"Ecophysiology of Fe-cycling bacteria in acidic sediments","volume":"76","author":"Lu","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"2023021402015660200_bib68","doi-asserted-by":"crossref","first-page":"2814","DOI":"10.1111\/j.1462-2920.2010.02251.x","article-title":"Acid-tolerant microaerophilic Fe(II)-oxidizing bacteria promote Fe(III)-accumulation in a fen","volume":"12","author":"L\u00fcdecke","year":"2010","journal-title":"Environ Microbiol"},{"key":"2023021402015660200_bib69","doi-asserted-by":"crossref","DOI":"10.1128\/mSystems.00553-19","article-title":"Validating the Cyc2 neutrophilic iron oxidation pathway using meta-omics of zetaproteobacteria iron mats at marine hydrothermal vents","volume-title":"Msystems","author":"McAllister","year":"2020"},{"key":"2023021402015660200_bib70","doi-asserted-by":"crossref","DOI":"10.1093\/femsec\/fiab143","article-title":"Organic carbon from graminoid roots as a driver of fermentation in a fen","volume":"97","author":"Meier","year":"2021","journal-title":"FEMS Microbiol Ecol"},{"key":"2023021402015660200_bib71","doi-asserted-by":"crossref","first-page":"995","DOI":"10.5194\/bg-11-995-2014","article-title":"The role of mixotrophic protists in the biological carbon pump","volume":"11","author":"Mitra","year":"2014","journal-title":"Biogeosciences"},{"key":"2023021402015660200_bib72","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.3389\/fmicb.2016.02082","article-title":"Reconstruction of the metabolic potential of acidophilic sideroxydans strains from the metagenome of an microaerophilic enrichment culture of acidophilic iron-oxidizing bacteria from a pilot plant for the treatment of acid mine drainage reveals metabolic versatility and adaptation to life at low pH","volume":"7","author":"M\u00fchling","year":"2016","journal-title":"Front Microbiol"},{"key":"2023021402015660200_bib73","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.49816","article-title":"Genomic adaptations in information processing underpin trophic strategy in a whole-ecosystem nutrient enrichment experiment","volume-title":"Elife","author":"Okie","year":"2020"},{"key":"2023021402015660200_bib74","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1111\/1462-2920.14260","article-title":"The distribution of active iron-cycling bacteria in marine and freshwater sediments is decoupled from geochemical gradients","volume":"20","author":"Otte","year":"2018","journal-title":"Environ Microbiol"},{"key":"2023021402015660200_bib75","doi-asserted-by":"crossref","first-page":"D206","DOI":"10.1093\/nar\/gkt1226","article-title":"The SEED and the rapid annotation of microbial genomes using Subsystems Technology (RAST)","volume":"42","author":"Overbeek","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"2023021402015660200_bib76","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1111\/1462-2920.13023","article-title":"Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples","volume":"18","author":"Parada","year":"2016","journal-title":"Environ Microbiol"},{"key":"2023021402015660200_bib77","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.01667-20","article-title":"Expanded diversity and metabolic versatility of marine nitrite-oxidizing bacteria revealed by cultivation- and genomics-based approaches","volume":"86","author":"Park","year":"2020","journal-title":"Appl Environ Microbiol"},{"key":"2023021402015660200_bib78","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/j.soilbio.2005.09.001","article-title":"Reduction processes in forest wetlands: tracking down heterogeneity of source\/sink functions with a combination of methods","volume":"38","author":"Paul","year":"2006","journal-title":"Soil Biol Biochem"},{"key":"2023021402015660200_bib79","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1038\/ismej.2010.75","article-title":"A \u2018rare biosphere\u2019 microorganism contributes to sulfate reduction in a peatland","volume":"4","author":"Pester","year":"2010","journal-title":"ISME J"},{"key":"2023021402015660200_bib80","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1016\/j.bbamcr.2014.11.021","article-title":"FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation","volume":"1853","author":"Peters","year":"2015","journal-title":"Biochimica Et Biophysica Acta (BBA) - Mol Cell Res"},{"key":"2023021402015660200_bib81","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1042\/bst0300653","article-title":"Cytochrome cbb(3) oxidase and bacterial microaerobic metabolism","volume":"30","author":"Pitcher","year":"2002","journal-title":"Biochem Soc Trans"},{"key":"2023021402015660200_bib82","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1574-6941.2008.00523.x","article-title":"Competition of Fe(III) reduction and methanogenesis in an acidic fen","volume":"65","author":"Reiche","year":"2008","journal-title":"FEMS Microbiol Ecol"},{"key":"2023021402015660200_bib83","doi-asserted-by":"crossref","first-page":"187","DOI":"10.5194\/bg-7-187-2010","article-title":"Effect of peat quality on microbial greenhouse gas formation in an acidic fen","volume":"7","author":"Reiche","year":"2010","journal-title":"Biogeosciences"},{"key":"2023021402015660200_bib84","doi-asserted-by":"crossref","first-page":"10101","DOI":"10.1073\/pnas.1221487110","article-title":"Iron traps terrestrially derived dissolved organic matter at redox interfaces","volume":"110","author":"Riedel","year":"2013","journal-title":"Proc Natl Acad Sci"},{"key":"2023021402015660200_bib85","first-page":"e1900v1","article-title":"The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes","volume-title":"PeerJ Preprints","author":"Rodriguez-R","year":"2016"},{"key":"2023021402015660200_bib86","doi-asserted-by":"crossref","first-page":"e2584","DOI":"10.7717\/peerj.2584","article-title":"VSEARCH: a versatile open source tool for metagenomics","volume":"4","author":"Rognes","year":"2016","journal-title":"PeerJ"},{"key":"2023021402015660200_bib87","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1038\/ismej.2014.235","article-title":"The physiology and ecological implications of efficient growth","volume":"9","author":"Roller","year":"2015","journal-title":"ISME J"},{"key":"2023021402015660200_bib98_1673251032633","article-title":"RStudio: Integrated Development for R. RStudio, PBC, Boston, MA URL","author":"RStudio","year":"2020"},{"key":"2023021402015660200_bib88","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1016\/j.bbabio.2013.01.015","article-title":"NiFe] hydrogenases: a common active site for hydrogen metabolism under diverse conditions","volume":"1827","author":"Shafaat","year":"2013","journal-title":"Biochimica Et Biophysica Acta (BBA) - Bioenergetics"},{"key":"2023021402015660200_bib89","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/1462-2920.14010","article-title":"Tracking active groundwater microbes with D2O labelling to understand their ecosystem function","volume":"20","author":"Taubert","year":"2018","journal-title":"Environ Microbiol"},{"key":"2023021402015660200_bib90","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1021\/es9707769","article-title":"Fe(0)-supported autotrophic denitrification","volume":"32","author":"Till","year":"1998","journal-title":"Environ Sci Technol"},{"key":"2023021402015660200_bib91","doi-asserted-by":"crossref","first-page":"e0146832","DOI":"10.1371\/journal.pone.0146832","article-title":"Genome analysis of the biotechnologically relevant acidophilic iron oxidising strain JA12 indicates phylogenetic and metabolic diversity within the novel genus \u201cFerrovum","volume":"11","author":"Ullrich","year":"2016","journal-title":"PLoS One"},{"key":"2023021402015660200_bib92","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/nrmicro1490","article-title":"Microorganisms pumping iron: anaerobic microbial iron oxidation and reduction","volume":"4","author":"Weber","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"2023021402015660200_bib93","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"Ggplot2: elegant graphics for Data analysis","author":"Wickham","year":"2016"},{"key":"2023021402015660200_bib94","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1111\/j.1462-2920.2009.01867.x","article-title":"Trophic links between fermenters and methanogens in a moderately acidic fen soil","volume":"11","author":"W\u00fcst","year":"2009","journal-title":"Environ Microbiol"},{"key":"2023021402015660200_bib95","doi-asserted-by":"crossref","first-page":"W52","DOI":"10.1093\/nar\/gkz333","article-title":"OrthoVenn2: a web server for whole-genome comparison and annotation of orthologous clusters across multiple species","volume":"47","author":"Xu","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2023021402015660200_bib96","doi-asserted-by":"crossref","first-page":"674411","DOI":"10.3389\/fmicb.2021.674411","article-title":"Geographical distribution of iron redox cycling bacterial community in peatlands: distinct assemble mechanism across environmental gradient","volume":"12","author":"Yang","year":"2021","journal-title":"Front Microbiol"},{"key":"2023021402015660200_bib97","doi-asserted-by":"crossref","first-page":"e0159521","DOI":"10.1128\/AEM.01595-21","article-title":"Unraveling Fe(II)-oxidizing mechanisms in a facultative Fe(II) oxidizer, Sideroxydans lithotrophicus strain ES-1, via culturing, transcriptomics, and reverse transcription-quantitative PCR","volume":"88","author":"Zhou","year":"2022","journal-title":"Appl Environ Microbiol"}],"container-title":["FEMS Microbiology Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/femsec\/advance-article-pdf\/doi\/10.1093\/femsec\/fiac156\/48584897\/fiac156.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsec\/article-pdf\/99\/2\/fiac156\/49172672\/fiac156.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsec\/article-pdf\/99\/2\/fiac156\/49172672\/fiac156.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,3]],"date-time":"2023-12-03T23:58:40Z","timestamp":1701647920000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsec\/article\/doi\/10.1093\/femsec\/fiac156\/6979798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,9]]},"references-count":98,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,1,24]]}},"URL":"https:\/\/doi.org\/10.1093\/femsec\/fiac156","relation":{},"ISSN":["1574-6941"],"issn-type":[{"value":"1574-6941","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,2]]},"published":{"date-parts":[[2023,1,9]]},"article-number":"fiac156"}}