{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T14:54:23Z","timestamp":1775228063536,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T00:00:00Z","timestamp":1686528000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Microbacterium sp. C448, isolated from a soil regularly exposed to sulfamethazine (SMZ), can use various sulphonamide antibiotics as the sole carbon source for growth. The basis for the regulation of genes encoding the sulphonamide metabolism pathway, the dihydropteroate synthase sulphonamide target (folP), and the sulphonamide resistance (sul1) genes, is unknown in this organism. In the present study, the response of the transcriptome and proteome of Microbacterium sp. C448 following exposure to subtherapeutic (33\u00a0\u00b5M) or therapeutic (832\u00a0\u00b5M) SMZ concentrations was evaluated. Therapeutic concentration induced the highest sad expression and Sad production, consistent with the activity of SMZ degradation observed in cellulo. Following complete SMZ degradation, Sad production tended to return to the basal level observed prior to SMZ exposure. Transcriptomic and proteomic kinetics were concomitant for the resistance genes and proteins. The abundance of Sul1 protein, 100-fold more abundant than FolP protein, did not change in response to SMZ exposure. Moreover, non-targeted analyses highlighted the increase of a deaminase RidA and a putative sulphate exporter expression and production. These two novel factors involved in the 4-aminophenol metabolite degradation and the export of sulphate residues formed during SMZ degradation, respectively, provided new insights into Microbacterium sp. C448 SMZ detoxification process.<\/jats:p>","DOI":"10.1093\/femsec\/fiad064","type":"journal-article","created":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T09:54:20Z","timestamp":1686390860000},"source":"Crossref","is-referenced-by-count":6,"title":["Effect of subtherapeutic and therapeutic sulfamethazine concentrations on transcribed genes and translated proteins involved in <i>Microbacterium<\/i> sp. C448 resistance and degradation"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8383-0365","authenticated-orcid":false,"given":"Laurianne","family":"Paris","sequence":"first","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne , CNRS, LMGE, F-63000 Clermont-Ferrand , France"},{"name":"Universit\u00e9 Clermont Auvergne , CNRS, ICCF, F-63000 Clermont-Ferrand , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Devers-Lamrani","sequence":"additional","affiliation":[{"name":"Institut Agro, INRAE, Universit\u00e9 de Bourgogne, Universit\u00e9 de Bourgogne Franche-Comt\u00e9 , Agro\u00e9cologie, F-21000 Dijon , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muriel","family":"Joly","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne , CNRS, LMGE, F-63000 Clermont-Ferrand , France"},{"name":"Universit\u00e9 Clermont Auvergne , CNRS, ICCF, F-63000 Clermont-Ferrand , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"Viala","sequence":"additional","affiliation":[{"name":"INRAE Site de Theix, Plate-forme d'exploration du m\u00e9tabolisme , F-63122 Saint-Gen\u00e8s Champanelle, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"De Antonio","sequence":"additional","affiliation":[{"name":"Biostatistics Unit (DRCI), Clermont-Ferrand University Hospital , F-63000 Clermont-Ferrand , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3778-7161","authenticated-orcid":false,"given":"Bruno","family":"Pereira","sequence":"additional","affiliation":[{"name":"Biostatistics Unit (DRCI), Clermont-Ferrand University Hospital , F-63000 Clermont-Ferrand , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Rouard","sequence":"additional","affiliation":[{"name":"Institut Agro, INRAE, Universit\u00e9 de Bourgogne, Universit\u00e9 de Bourgogne Franche-Comt\u00e9 , Agro\u00e9cologie, F-21000 Dijon , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale","family":"Besse-Hoggan","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne , CNRS, ICCF, F-63000 Clermont-Ferrand , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"H\u00e9braud","sequence":"additional","affiliation":[{"name":"INRAE Site de Theix, Plate-forme d'exploration du m\u00e9tabolisme , F-63122 Saint-Gen\u00e8s Champanelle, France"},{"name":"Universit\u00e9 Clermont Auvergne, INRAE, UMR MEDiS , F-63122 Saint-Gen\u00e8s Champanelle, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Topp","sequence":"additional","affiliation":[{"name":"London Research and Development Centre, Agriculture and Agri-Food Canada , London, ON N5V 4T3 , Canada"},{"name":"Department of Biology, University of Western Ontario , London, ON N6A 3K7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrice","family":"Martin-Laurent","sequence":"additional","affiliation":[{"name":"Institut Agro, INRAE, Universit\u00e9 de Bourgogne, Universit\u00e9 de Bourgogne Franche-Comt\u00e9 , Agro\u00e9cologie, F-21000 Dijon , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Batisson","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne , CNRS, LMGE, F-63000 Clermont-Ferrand , France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,6,12]]},"container-title":["FEMS Microbiology Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/femsec\/advance-article-pdf\/doi\/10.1093\/femsec\/fiad064\/50576897\/fiad064.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsec\/advance-article-pdf\/doi\/10.1093\/femsec\/fiad064\/50576897\/fiad064.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T04:06:50Z","timestamp":1686629210000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsec\/advance-article\/doi\/10.1093\/femsec\/fiad064\/7194726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,12]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/femsec\/fiad064","relation":{},"ISSN":["0168-6496","1574-6941"],"issn-type":[{"value":"0168-6496","type":"print"},{"value":"1574-6941","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,12]]},"article-number":"fiad064"}}