{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:19:57Z","timestamp":1787008797429,"version":"build-2736575974"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Netherlands Organisation for Scientific Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017334","name":"Soehngen Institute of Anaerobic Microbiology","doi-asserted-by":"publisher","award":["024002002"],"award-info":[{"award-number":["024002002"]}],"id":[{"id":"10.13039\/100017334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"ERC","doi-asserted-by":"publisher","award":["8540088"],"award-info":[{"award-number":["8540088"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aquatic ecosystems are large contributors to global methane (CH4) emissions. Eutrophication significantly enhances CH4-production as it stimulates methanogenesis. Mitigation measures aimed at reducing eutrophication, such as the addition of metal salts to immobilize phosphate (PO43\u2212), are now common practice. However, the effects of such remedies on methanogenic and methanotrophic communities\u2014and therefore on CH4-cycling\u2014remain largely unexplored. Here, we demonstrate that Fe(II)Cl2 addition, used as PO43- binder, differentially affected microbial CH4 cycling-processes in field experiments and batch incubations. In the field experiments, carried out in enclosures in a eutrophic pond, Fe(II)Cl2 application lowered in-situ CH4 emissions by lowering net CH4-production, while sediment aerobic CH4-oxidation rates\u2014as found in batch incubations of sediment from the enclosures\u2014did not differ from control. In Fe(II)Cl2-treated sediments, a decrease in net CH4-production rates could be attributed to the stimulation of iron-dependent anaerobic CH4-oxidation (Fe-AOM). In batch incubations, anaerobic CH4-oxidation and Fe(II)-production started immediately after CH4 addition, indicating Fe-AOM, likely enabled by favorable indigenous iron cycling conditions and the present methanotroph community in the pond sediment. 16S rRNA sequencing data confirmed the presence of anaerobic CH4-oxidizing archaea and both iron-reducing and iron-oxidizing bacteria in the tested sediments. Thus, besides combatting eutrophication, Fe(II)Cl2 application can mitigate CH4 emissions by reducing microbial net CH4-production and stimulating Fe-AOM.<\/jats:p>","DOI":"10.1093\/femsec\/fiae061","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T20:04:38Z","timestamp":1713297878000},"source":"Crossref","is-referenced-by-count":6,"title":["Fe(II)Cl2 amendment suppresses pond methane emissions by stimulating iron-dependent anaerobic oxidation of methane"],"prefix":"10.1093","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7404-6963","authenticated-orcid":false,"given":"Quinten","family":"Struik","sequence":"first","affiliation":[{"name":"Department of Ecology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R","family":"Parana\u00edba","sequence":"additional","affiliation":[{"name":"Department of Ecology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martyna","family":"Glodowska","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarian","family":"Kosten","sequence":"additional","affiliation":[{"name":"Department of Ecology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berber M J W","family":"Meulepas","sequence":"additional","affiliation":[{"name":"Department of Ecology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana B","family":"Rios-Miguel","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike S M","family":"Jetten","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miquel","family":"L\u00fcrling","sequence":"additional","affiliation":[{"name":"Aquatic Ecology & Water Quality Management Group, Department of Environmental Sciences, Wageningen University , PO Box 47, 6700 AA, Wageningen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Waajen","sequence":"additional","affiliation":[{"name":"Water Authority Brabantse Delta , 4836 AA, Breda , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P A","family":"Nijman","sequence":"additional","affiliation":[{"name":"Department of Ecology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6286-7484","authenticated-orcid":false,"given":"Annelies J","family":"Veraart","sequence":"additional","affiliation":[{"name":"Department of Ecology, Radboud Institute for Biological and Environmental Sciences, Radboud University , 6525 AJ, Nijmegen , The 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