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Here, we aimed to isolate and characterize novel thermophilic acetogens from diverse environments. Enrichment of heterotrophic and autotrophic acetogens was monitored by 16S rRNA gene-based bacterial community analysis. Seven novel Moorella strains were isolated and characterized by genomic and physiological analyses. Two Moorella humiferrea isolates showed considerable differences during autotrophic growth. The M. humiferrea LNE isolate (DSM 117358) fermented carbon monoxide (CO) to acetate, while the M. humiferrea OCP isolate (DSM 117359) transformed CO to hydrogen and carbon dioxide (H2 + CO2), employing the water\u2013gas shift reaction. Another carboxydotrophic hydrogenogenic Moorella strain was isolated from the covering soil of an active charcoal burning pile and proposed as the type strain (ACPsT) of the novel species Moorella carbonis (DSM 116161T and CCOS 2103T). The remaining four novel strains were affiliated with Moorella thermoacetica and showed, together with the type strain DSM 2955T, the production of small amounts of ethanol from H2 + CO2 in addition to acetate. The physiological analyses of the novel Moorella strains revealed isolate-specific differences that considerably increase the knowledge base on thermophilic acetogens for future applications.<\/jats:p>","DOI":"10.1093\/femsec\/fiae109","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T01:31:10Z","timestamp":1723080670000},"source":"Crossref","is-referenced-by-count":12,"title":["Isolation and characterization of novel acetogenic <i>Moorella<\/i> strains for employment as potential thermophilic biocatalysts"],"prefix":"10.1093","volume":"100","author":[{"given":"Tim","family":"B\u00f6er","sequence":"first","affiliation":[{"name":"Genomic and Applied Microbiology and G\u00f6ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University G\u00f6ttingen , 37077 G\u00f6ttingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Engelhardt","sequence":"additional","affiliation":[{"name":"Microbiology, Institute of Biological Sciences, University Rostock , 18059 Rostock , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"L\u00fcschen","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology and G\u00f6ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University G\u00f6ttingen , 37077 G\u00f6ttingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Eysell","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology and G\u00f6ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University G\u00f6ttingen , 37077 G\u00f6ttingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Environmental Biotechnology Group, Department of Geosciences, University of T\u00fcbingen , 72074 T\u00fcbingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Schneider","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology and G\u00f6ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University G\u00f6ttingen , 37077 G\u00f6ttingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Largus T","family":"Angenent","sequence":"additional","affiliation":[{"name":"Environmental Biotechnology Group, Department of Geosciences, University of T\u00fcbingen , 72074 T\u00fcbingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirko","family":"Basen","sequence":"additional","affiliation":[{"name":"Microbiology, Institute of Biological Sciences, University Rostock , 18059 Rostock , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8646-7925","authenticated-orcid":false,"given":"Rolf","family":"Daniel","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology and G\u00f6ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University G\u00f6ttingen , 37077 G\u00f6ttingen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2473-6202","authenticated-orcid":false,"given":"Anja","family":"Poehlein","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology and G\u00f6ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University 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