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The analysis of shield bug microbiomes has been mainly focused on the gut intestinal tract predominantly colonized by Pantoea symbionts and other microbial community members in the gut or other organs have hardly been investigated. In this study, we reveal that the Southern green shield bug Nezara viridula harbours a Sodalis symbiont in several organs, with a notable prevalence in salivary glands, and anterior regions of the midgut. Removing external egg microbiota via sterilization profoundly impacted insect viability but did not disrupt the vertical transmission of Sodalis and Pantoea symbionts. Based on the dominance of Sodalis in testes, we deduce that N. viridula males could be involved in symbiont vertical transmission. Genomic analyses comparing Sodalis species revealed that Sodalis sp. Nvir shares characteristics with both free-living and obligate insect-associated Sodalis spp. Sodalis sp. Nvir also displays genome instability typical of endosymbiont lineages, which suggests ongoing speciation to an obligate endosymbiont. Together, our study reveals that shield bugs harbour unrecognized symbionts that might be paternally transmitted.<\/jats:p>","DOI":"10.1093\/femsec\/fiaf017","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T07:16:18Z","timestamp":1739258178000},"source":"Crossref","is-referenced-by-count":4,"title":["From eggs to guts: Symbiotic association of\n                    <i>Sodalis nezarae<\/i>\n                    sp. nov. with the Southern green shield bug\n                    <i>Nezara viridula<\/i>"],"prefix":"10.1093","volume":"101","author":[{"given":"Magda A","family":"Rogowska-van\u00a0der\u00a0Molen","sequence":"first","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , PO Box 9010, 6500 Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Manzano-Mar\u00edn","sequence":"additional","affiliation":[{"name":"Centre for Microbiology and Environmental Systems Science, University of Vienna , Djerassiplatz 1, 1030 Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelle L","family":"Postma","sequence":"additional","affiliation":[{"name":"Department of General Instrumentation, Faculty of Science, Radboud University , Heyendaalseweg 135, 6525 Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Coolen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , PO Box 9010, 6500 Nijmegen ,","place":["The Netherlands"]},{"name":"Translational Plant Biology, Department of Biology, Utrecht University , PO Box 800.56, 3508\u00a0Utrecht ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"van\u00a0Alen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , PO Box 9010, 6500 Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , PO Box 9010, 6500 Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1568-8878","authenticated-orcid":false,"given":"Cornelia U","family":"Welte","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Radboud University , PO 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