{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T19:44:21Z","timestamp":1788291861452,"version":"build-2803163510"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"21","license":[{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100006143","name":"Department of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006143","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["CRG\/2019\/001755"],"award-info":[{"award-number":["CRG\/2019\/001755"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005965","name":"NASI","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005965","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Helicobacter pylori, a type 1 carcinogen, accounts for numerous gastric cancer-related deaths worldwide. Repurposing existing drugs or developing new ones for a combinatorial approach against increasing antimicrobial resistance is the need of the hour. This study highlights the efficacy of acriflavine hydrochloride (ACF-HCl) in inhibiting the growth of H. pylori reference strain and antibiotic-resistant clinical isolates at low concentrations. ACF-HCl inhibits H. pylori growth at MIC value 10 times less than that in Escherichia coli, another Gram-negative bacteria. Furthermore, ACF-HCl demonstrates synergistic effect with clarithromycin, a commonly used antibiotic against H. pylori. ACF-HCl treatment also eradicates H. pylori infection in the mice model efficiently. Our in vitro data indicate that bacterial membrane is the prime target. The novel action of ACF-HCl against antibiotic-resistant clinical isolates, synergistic effect with the conventional antibiotic clarithromycin and eradication of H. pylori from infected mice highlight the potential of ACF-HCl as a promising therapeutic agent against H. pylori by itself as well as for combinatorial therapy.<\/jats:p>","DOI":"10.1093\/femsle\/fnaa178","type":"journal-article","created":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T11:12:37Z","timestamp":1603537957000},"source":"Crossref","is-referenced-by-count":13,"title":["Antibacterial action of acriflavine hydrochloride for eradication of the gastric pathogen <i>Helicobacter pylori<\/i>"],"prefix":"10.1093","volume":"367","author":[{"given":"Ankita","family":"Tehlan","sequence":"first","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University (JNU), New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bipul 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