{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:57:41Z","timestamp":1753876661790,"version":"3.41.2"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T00:00:00Z","timestamp":1611014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100010834","name":"Education Department of Hainan Province","doi-asserted-by":"publisher","award":["Hnky2019ZD-27"],"award-info":[{"award-number":["Hnky2019ZD-27"]}],"id":[{"id":"10.13039\/501100010834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["820RC650","81760376","82060377","81900386"],"award-info":[{"award-number":["820RC650","81760376","82060377","81900386"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7192240"],"award-info":[{"award-number":["7192240"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,2,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Streptococcus pneumoniae is an important clinical pathogenic bacterium that is the primary cause of meningitis, septicemia and community-acquired pneumonia. The mortality rate of pneumococcal disease is high, especially in children younger than 5-years-old. Rapid and accurate detection of S.pneumoniae is critical for clinical diagnosis. A ply gene-based multiple cross displacement amplification (MCDA) assay, amplifying DNA under 65\u00b0C\u00a0for 40\u00a0min, was established to detect S.pneumoniae. Antarctic thermal sensitive uracil-DNA-glycosylase (AUDG) was applied to prevent carryover contamination. A lateral flow biosensor (LFB) was used to indicate the MCDA results. The ply-MCDA assay could detect as low as 10 fg of S. pneumoniae DNA and 447 colony forming units (CFU)\/mL of spiked sputum samples. The analytical sensitivity of the ply-MCDA assay to detect clinical specimens was 100 times higher than that of PCR. The specificity of the ply-MCDA assay was evaluated using 15 S.pneumoniae strains and 25 non-S. pneumoniae strains, which confirmed the high selectivity of the ply-MCDA assay for S.pneumoniae. The AUDG enzyme could effectively eliminate carryover contamination and thus prevented false-positive results. In conclusion, ply-AUDG-MCDA-LFB is a simple, rapid and accurate method to detect S.pneumoniae.<\/jats:p>","DOI":"10.1093\/femsle\/fnab006","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T12:19:48Z","timestamp":1610713188000},"source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous detection of <i>Streptococcus<\/i>\n                  <i>pneumoniae<\/i> and prevention of carryover contamination using multiple cross displacement amplification with Antarctic thermal sensitive uracil-DNA-glycosylase and a lateral flow biosensor"],"prefix":"10.1093","volume":"368","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0008-8914","authenticated-orcid":false,"given":"Linlin","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Peking University Shougang Hospital, No. 9 Jinyuanzhuang Road, Shijingshan District, Beijing 100144, 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