{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T21:18:26Z","timestamp":1771535906018,"version":"3.50.1"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","award":["2018ZX10711001-003"],"award-info":[{"award-number":["2018ZX10711001-003"]}],"id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFC1200501"],"award-info":[{"award-number":["2018YFC1200501"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03\u2013500\u00a0ng\/mL. The half-maximal inhibitory concentration was 2.28\u00a0ng\/mL and the coefficient of variation was &amp;lt;10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1\u00a0ng\/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.<\/jats:p>","DOI":"10.1093\/femsle\/fnab029","type":"journal-article","created":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T12:10:09Z","timestamp":1616155809000},"source":"Crossref","is-referenced-by-count":11,"title":["A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA"],"prefix":"10.1093","volume":"368","author":[{"given":"Liwen","family":"Zhang","sequence":"first","affiliation":[{"name":"Anhui Medical University, Hefei, Anhui 230032, China"},{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyu","family":"Lv","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuling","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Decong","family":"Kong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhua","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7587-830X","authenticated-orcid":false,"given":"Yongqiang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Anhui Medical University, Hefei, Anhui 230032, China"},{"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,3,23]]},"reference":[{"key":"2021070721375564100_bib1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.2174\/1875397300801010002","article-title":"The use of AlphaScreen technology in HTS: current status","volume":"1","author":"Eglen","year":"2008","journal-title":"Curr Chem Genomics"},{"key":"2021070721375564100_bib2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.fct.2015.02.005","article-title":"In vivo toxicity studies of fusarium mycotoxins in the last decade: a review","volume":"78","author":"Escriva","year":"2015","journal-title":"Food Chem Toxicol"},{"key":"2021070721375564100_bib3","doi-asserted-by":"crossref","first-page":"1177271918757484","DOI":"10.1177\/1177271918757484","article-title":"A homogeneous time-resolved fluorescence immunoassay method for the measurement of compound W","volume":"13","author":"Huang","year":"2018","journal-title":"Biomark Insights"},{"key":"2021070721375564100_bib4","doi-asserted-by":"crossref","first-page":"9763","DOI":"10.1021\/ac801875a","article-title":"Homogeneous dual-parameter assay for prostate-specific antigen based on fluorescence resonance energy transfer","volume":"80","author":"Kokko","year":"2008","journal-title":"Anal Chem"},{"key":"2021070721375564100_bib5","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.cca.2013.09.013","article-title":"Development of an amplified luminescent proximity homogeneous assay for quantitative determination of hepatitis B surface antigen in human serum","volume":"426","author":"Liu","year":"2013","journal-title":"Clin Chim Acta"},{"key":"2021070721375564100_bib6","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.talanta.2016.11.032","article-title":"Development of a nanoarray capable of the rapid and simultaneous detection of zearalenone, T2-toxin and fumonisin","volume":"164","author":"Mcnamee","year":"2017","journal-title":"Talanta"},{"key":"2021070721375564100_bib7","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1007\/s00216-013-6752-1","article-title":"A novel homogeneous immunoassay for anthrax detection based on the AlphaLISA method: detection of B. anthracis spores and protective antigen (PA) in complex samples","volume":"405","author":"Mechaly","year":"2013","journal-title":"Anal Bioanal Chem"},{"key":"2021070721375564100_bib8","doi-asserted-by":"crossref","first-page":"247","DOI":"10.3390\/toxins8080247","article-title":"Occurrence of Fusarium langsethiae and T-2 and HT-2 toxins in Italian malting barley","volume":"8","author":"Morcia","year":"2016","journal-title":"Toxins (Basel)"},{"key":"2021070721375564100_bib9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1297-9716-43-35","article-title":"The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig","volume":"43","author":"Seeboth","year":"2012","journal-title":"Vet Res"},{"key":"2021070721375564100_bib10","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.ijcard.2014.12.052","article-title":"A multimarker approach to diagnose and stratify heart failure","volume":"181","author":"Wan","year":"2015","journal-title":"Int J Cardiol"},{"key":"2021070721375564100_bib12","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.toxicon.2020.02.023","article-title":"Distribution and persistence of residual T-2 and HT-2 toxins from moldy feed in broiler chickens","volume":"178","author":"Yang","year":"2020","journal-title":"Toxicon"},{"key":"2021070721375564100_bib11","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1002\/cam4.1449","article-title":"A novel rapid quantitative method reveals stathmin-1 as a promising marker for esophageal squamous cell carcinoma","volume":"7","author":"Yan","year":"2018","journal-title":"Cancer Med"},{"key":"2021070721375564100_bib13","doi-asserted-by":"crossref","first-page":"127912","DOI":"10.1016\/j.snb.2020.127912","article-title":"Ultrasensitive and rapid detection of T-2 toxin using a target-responsive DNA hydrogel","volume":"311","author":"Yunfeng\u00a0Sun","year":"2020","journal-title":"Sens Actuators B Chem"},{"key":"2021070721375564100_bib14","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.aca.2018.09.007","article-title":"Ultrasensitive detection of T-2 toxin in food based on bio-barcode and rolling circle amplification","volume":"1043","author":"Zhang","year":"2018","journal-title":"Anal Chim Acta"},{"key":"2021070721375564100_bib15","doi-asserted-by":"crossref","first-page":"1944","DOI":"10.1002\/jsfa.5566","article-title":"Development of a homogeneous immunoassay based on the AlphaLISA method for the detection of chloramphenicol in milk, honey and eggs","volume":"92","author":"Zhang","year":"2012","journal-title":"J Sci Food Agric"},{"key":"2021070721375564100_bib16","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.toxicon.2019.04.016","article-title":"AlphaLISA for detection of staphylococcal enterotoxin B free from interference by protein A","volume":"165","author":"Zhao","year":"2019","journal-title":"Toxicon"},{"key":"2021070721375564100_bib17","doi-asserted-by":"crossref","first-page":"111635","DOI":"10.1016\/j.bios.2019.111635","article-title":"A novel sandwich aptasensor for detecting T-2 toxin based on rGO-TEPA-Au@Pt nanorods with a dual signal amplification strategy","volume":"144","author":"Zhong","year":"2019","journal-title":"Biosens Bioelectron"}],"container-title":["FEMS Microbiology Letters"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femsle\/advance-article-pdf\/doi\/10.1093\/femsle\/fnab029\/36676856\/fnab029.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsle\/article-pdf\/368\/6\/fnab029\/37301625\/fnab029.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsle\/article-pdf\/368\/6\/fnab029\/37301625\/fnab029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T23:01:34Z","timestamp":1625698894000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsle\/article\/doi\/10.1093\/femsle\/fnab029\/6184045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,4,22]]}},"URL":"https:\/\/doi.org\/10.1093\/femsle\/fnab029","relation":{},"ISSN":["1574-6968"],"issn-type":[{"value":"1574-6968","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,3]]},"published":{"date-parts":[[2021,3]]},"article-number":"fnab029"}}