{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T19:07:27Z","timestamp":1790276847930,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T00:00:00Z","timestamp":1631145600000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"USDA","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["1011114"],"award-info":[{"award-number":["1011114"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["1018898"],"award-info":[{"award-number":["1018898"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2019-67017-29687"],"award-info":[{"award-number":["2019-67017-29687"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Listeria monocytogenes is the causative agent of the highly fatal foodborne disease listeriosis and can persist in food production environments. Recent research highlights the involvement of L. monocytogenes plasmids in different stress response mechanisms, which contribute to its survival in food production facilities. Ultraviolet (UV) light in the UVC spectrum (200\u2013280\u00a0nm) is used in food production to control microbial contamination. Although plasmid-encoded UV resistance mechanisms have been described in other bacteria, no research indicates that L. monocytogenes plasmids contribute to the UV stress response. The plasmids of L. monocytogenes strains 6179, 4KSM and R479a are genetically distinct and were utilized to study the roles of plasmids in the UV response. Wild-type and plasmid-cured variant cells were grown to logarithmic or late-stationary phase, plated on agar plates and exposed to UVC for 60 or 90\u00a0s, and colony-forming units (CFUs) were determined. CFUs of 6179 and 4KSM, bearing pLM6179 and p4KSM, respectively, were significantly (P-value &amp;lt;\u00a00.05) higher than those of the plasmid-cured strains in both logarithmic and stationary phases. No difference in survival was observed for the R479a strain. Our data show for the first time that certain L. monocytogenes plasmids contribute to the survival of UVC light stress.<\/jats:p>","DOI":"10.1093\/femsle\/fnab123","type":"journal-article","created":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T11:34:09Z","timestamp":1631100849000},"source":"Crossref","is-referenced-by-count":9,"title":["Certain <i>Listeria monocytogenes<\/i> plasmids contribute to increased UVC ultraviolet light stress"],"prefix":"10.1093","volume":"368","author":[{"given":"Justin M","family":"Anast","sequence":"first","affiliation":[{"name":"Department of Animal Science, Iowa State University, Ames, IA 50011, USA"},{"name":"Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA 50011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1907-0709","authenticated-orcid":false,"given":"Stephan","family":"Schmitz-Esser","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Iowa State University, Ames, IA 50011, USA"},{"name":"Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA 50011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"key":"2021092302143895600_bib1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.ijfoodmicro.2015.06.018","article-title":"Ultraviolet-C light inactivation of Escherichia coli O157:H7 and Listeria monocytogenes on organic fruit surfaces","volume":"210","author":"Adhikari","year":"2015","journal-title":"Int J Food Microbiol"},{"key":"2021092302143895600_bib2","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ijfoodmicro.2011.03.009","article-title":"Listeria monocytogenes survival of UV-C radiation is enhanced by presence of sodium chloride, organic food material and by bacterial biofilm formation","volume":"147","author":"Bernbom","year":"2011","journal-title":"Int J Food Microbiol"},{"key":"2021092302143895600_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.foodcont.2016.12.016","article-title":"A review of Listeria monocytogenes: an update on outbreaks, virulence, dose-response, ecology, and risk assessments","volume":"75","author":"Buchanan","year":"2017","journal-title":"Food Control"},{"key":"2021092302143895600_bib4","doi-asserted-by":"crossref","first-page":"2700","DOI":"10.3389\/fmicb.2018.02700","article-title":"Resistance of Listeria monocytogenes to stress conditions encountered in food and food processing environments","volume":"9","author":"Bucur","year":"2018","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2011.01.005","article-title":"Review: persistence of Listeria monocytogenes in food industry equipment and premises","volume":"145","author":"Carpentier","year":"2011","journal-title":"Int J Food Microbiol"},{"key":"2021092302143895600_bib6","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3389\/fmicb.2014.00068","article-title":"Transcriptome analysis of Listeria monocytogenes exposed to biocide stress reveals a multi-system response involving cell wall synthesis, sugar uptake, and motility","volume":"5","author":"Casey","year":"2014","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib7","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s00248-007-9346-7","article-title":"62-kb plasmids harboring rulAB homologues confer UV-tolerance and epiphytic fitness to Pseudomonas syringae pv. syringae mango isolates","volume":"56","author":"Cazorla","year":"2008","journal-title":"Microb Ecol"},{"key":"2021092302143895600_bib8","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1080\/10408390701856272","article-title":"Physiology and genetics of Listeria monocytogenes survival and growth at cold temperatures","volume":"49","author":"Chan","year":"2009","journal-title":"Crit Rev Food Sci Nutr"},{"key":"2021092302143895600_bib9","doi-asserted-by":"crossref","first-page":"3110","DOI":"10.3389\/fmicb.2019.03110","article-title":"Transcriptome sequencing of Listeria monocytogenes reveals major gene expression changes in response to lactic acid stress exposure but a less pronounced response to oxidative stress","volume":"10","author":"Cortes","year":"2020","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib10","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0168-1605(00)00305-6","article-title":"Analysis of the role of the Listeria monocytogenes F0F1-ATPase operon in the acid tolerance response","volume":"60","author":"Cotter","year":"2000","journal-title":"Int J Food Microbiol"},{"key":"2021092302143895600_bib11","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1746-1448-2-11","article-title":"The uvrA, uvrB and uvrC genes are required for repair of ultraviolet light induced DNA photoproducts in Halobacterium sp. NRC-1","volume":"2","author":"Crowley","year":"2006","journal-title":"Saline Syst"},{"key":"2021092302143895600_bib12","doi-asserted-by":"crossref","first-page":"8231","DOI":"10.1128\/AEM.02056-10","article-title":"Genetic characterization of plasmid-associated benzalkonium chloride resistance determinants in a Listeria monocytogenes strain from the 1998\u20131999 outbreak","volume":"76","author":"Elhanafi","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"2021092302143895600_bib13","doi-asserted-by":"crossref","first-page":"e0151117","DOI":"10.1371\/journal.pone.0151117","article-title":"Genome analysis of Listeria monocytogenes sequence type 8 strains persisting in salmon and poultry processing environments and comparison with related strains","volume":"11","author":"Fagerlund","year":"2016","journal-title":"PLoS One"},{"key":"2021092302143895600_bib14","doi-asserted-by":"crossref","first-page":"e112649","DOI":"10.1371\/journal.pone.0112649","article-title":"Divergent evolution of the activity and regulation of the glutamate decarboxylase systems in Listeria monocytogenes EGD-e and 10403S: roles in virulence and acid tolerance","volume":"9","author":"Feehily","year":"2014","journal-title":"PLoS One"},{"key":"2021092302143895600_bib15","doi-asserted-by":"crossref","first-page":"150","DOI":"10.4315\/0362-028X.JFP-13-150","article-title":"Listeria monocytogenes persistence in food-associated environments: epidemiology, strain characteristics, and implications for public health","volume":"77","author":"Ferreira","year":"2014","journal-title":"J Food Prot"},{"key":"2021092302143895600_bib16","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.3389\/fmicb.2016.02057","article-title":"Comparative genomics of the Listeria monocytogenes ST204 subgroup","volume":"7","author":"Fox","year":"2016","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib17","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0022-2836(03)00615-6","article-title":"Electrostatic interactions in a peptide\u2013RNA complex","volume":"331","author":"Garc\u00eda-Garc\u00eda","year":"2003","journal-title":"J Mol Biol"},{"key":"2021092302143895600_bib18","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.fm.2014.08.011","article-title":"Environmental and biological factors influencing the UV-C resistance of Listeria monocytogenes","volume":"46","author":"Gayan","year":"2015","journal-title":"Food Microbiol"},{"key":"2021092302143895600_bib19","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.tifs.2007.03.010","article-title":"Pulsed light for food decontamination: a review","volume":"18","author":"Gomez-Lopez","year":"2007","journal-title":"Trends Food Sci Technol"},{"key":"2021092302143895600_bib20","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.ifset.2018.03.019","article-title":"Inactivation of Escherichia coli, Listeria and Salmonella by single and multiple wavelength ultraviolet-light emitting diodes","volume":"47","author":"Green","year":"2018","journal-title":"Innov Food Sci Emerg Technol"},{"key":"2021092302143895600_bib21","doi-asserted-by":"crossref","first-page":"e00827","DOI":"10.1128\/AEM.00827-17","article-title":"Stress survival islet 2, predominantly present in Listeria monocytogenes strains of sequence type 121, is involved in the alkaline and oxidative stress responses","volume":"83","author":"Harter","year":"2017","journal-title":"Appl Environ Microbiol"},{"key":"2021092302143895600_bib22","doi-asserted-by":"crossref","first-page":"426","DOI":"10.3390\/toxins11070426","article-title":"Comparative analysis of Listeria monocytogenes plasmids and expression levels of plasmid-encoded genes during growth under salt and acid stress conditions","volume":"11","author":"Hingston","year":"2019","journal-title":"Toxins"},{"key":"2021092302143895600_bib23","doi-asserted-by":"crossref","first-page":"M431","DOI":"10.1111\/j.1750-3841.2009.01319.x","article-title":"Inactivation of Listeria monocytogenes on unpackaged and vacuum-packaged chicken frankfurters using pulsed UV-light","volume":"74","author":"Keklik","year":"2009","journal-title":"J Food Sci"},{"key":"2021092302143895600_bib24","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.4315\/0362-028X-69.12.3031","article-title":"Role of uvrA in the growth and survival of Listeria monocytogenes under UV radiation and acid and bile stress","volume":"69","author":"Kim","year":"2006","journal-title":"J Food Prot"},{"key":"2021092302143895600_bib25","doi-asserted-by":"crossref","first-page":"e12511","DOI":"10.1371\/journal.pone.0012511","article-title":"Comparative analysis of plasmids in the genus Listeria","volume":"5","author":"Kuenne","year":"2010","journal-title":"PLoS One"},{"key":"2021092302143895600_bib26","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/36786","article-title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis","volume":"390","author":"Kunst","year":"1997","journal-title":"Nature"},{"key":"2021092302143895600_bib27","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1111\/1574-6976.12076","article-title":"Coping with low pH: molecular strategies in neutralophilic bacteria","volume":"38","author":"Lund","year":"2014","journal-title":"FEMS Microbiol Rev"},{"key":"2021092302143895600_bib28","doi-asserted-by":"crossref","first-page":"D412","DOI":"10.1093\/nar\/gkaa913","article-title":"Pfam: the protein families database in 2021","volume":"49","author":"Mistry","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2021092302143895600_bib29","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.3389\/fmicb.2018.01965","article-title":"The hurdle approach: a holistic concept for controlling food safety risks associated with pathogenic bacterial contamination of leafy green vegetables. A review","volume":"9","author":"Mogren","year":"2018","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib30","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3389\/fmicb.2018.00064","article-title":"New aspects on Listeria monocytogenes ST5-ECVI predominance in a heavily contaminated cheese processing environment","volume":"9","author":"Muhterem-Uyar","year":"2018","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib32","doi-asserted-by":"crossref","first-page":"e76835","DOI":"10.1371\/journal.pone.0076835","article-title":"Tn6188: a novel transposon in Listeria monocytogenes responsible for tolerance to benzalkonium chloride","volume":"8","author":"Muller","year":"2013","journal-title":"PLoS One"},{"key":"2021092302143895600_bib31","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1111\/1574-6968.12626","article-title":"The Listeria monocytogenes transposon Tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide","volume":"361","author":"Muller","year":"2014","journal-title":"FEMS Microbiol Lett"},{"key":"2021092302143895600_bib33","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.ijfoodmicro.2019.03.016","article-title":"Plasmids contribute to food processing environment-associated stress survival in three Listeria monocytogenes ST121, ST8, and ST5 strains","volume":"299","author":"Naditz","year":"2019","journal-title":"Int J Food Microbiol"},{"key":"2021092302143895600_bib34","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1111\/j.1365-2621.2005.01071.x","article-title":"Inactivation of Escherichia coli O157:H7 and Listeria monocytogenes inoculated on raw salmon fillets by pulsed UV-light treatment","volume":"41","author":"Ozer","year":"2006","journal-title":"Int J Food Sci Technol"},{"key":"2021092302143895600_bib35","doi-asserted-by":"crossref","first-page":"e00364","DOI":"10.1128\/mSphere.00364-17","article-title":"Heat resistance mediated by pLM58 plasmid-borne ClpL in Listeria monocytogenes","volume":"2","author":"Pontinen","year":"2017","journal-title":"mSphere"},{"key":"2021092302143895600_bib36","doi-asserted-by":"crossref","first-page":"592980","DOI":"10.4061\/2010\/592980","article-title":"Molecular mechanisms of ultraviolet radiation-induced DNA damage and repair","volume":"2010","author":"Rastogi","year":"2010","journal-title":"J Nucleic Acids"},{"key":"2021092302143895600_bib37","doi-asserted-by":"crossref","first-page":"e0176857","DOI":"10.1371\/journal.pone.0176857","article-title":"Comparative genomics of human and non-human Listeria monocytogenes sequence type 121 strains","volume":"12","author":"Rychli","year":"2017","journal-title":"PLoS One"},{"key":"2021092302143895600_bib38","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3201\/eid1701.P11101","article-title":"Foodborne illness acquired in the United States: major pathogens","volume":"17","author":"Scallan","year":"2011","journal-title":"Emerg Infect Dis"},{"key":"2021092302143895600_bib40","doi-asserted-by":"crossref","first-page":"653155","DOI":"10.3389\/fmicb.2021.653155","article-title":"A large-scale sequencing-based survey of plasmids in Listeria monocytogenes reveals global dissemination of plasmids","volume":"12","author":"Schmitz-Esser","year":"2021","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib39","doi-asserted-by":"crossref","first-page":"e00150","DOI":"10.1128\/genomeA.00150-15","article-title":"Complete genome sequence of the persistent Listeria monocytogenes strain R479a","volume":"3","author":"Schmitz-Esser","year":"2015","journal-title":"Genome Announc"},{"key":"2021092302143895600_bib41","doi-asserted-by":"crossref","first-page":"380","DOI":"10.3389\/fmicb.2015.00380","article-title":"Genomes of sequence type 121 Listeria monocytogenes strains harbor highly conserved plasmids and prophages","volume":"6","author":"Schmitz-Esser","year":"2015","journal-title":"Front Microbiol"},{"key":"2021092302143895600_bib42","doi-asserted-by":"crossref","first-page":"5272","DOI":"10.1128\/AEM.01354-13","article-title":"pA506, a conjugative plasmid of the plant epiphyte Pseudomonas fluorescens A506","volume":"79","author":"Stockwell","year":"2013","journal-title":"Appl Environ Microbiol"},{"key":"2021092302143895600_bib43","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0378-1119(96)00273-9","article-title":"Resistance to ultraviolet light in Pseudomonas syringae: sequence and functional analysis of the plasmid-encoded rulAB genes","volume":"177","author":"Sundin","year":"1996","journal-title":"Gene"},{"key":"2021092302143895600_bib44","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1128\/JB.185.7.2153-2160.2003","article-title":"Roles of YqjH and YqjW, homologs of the Escherichia coli UmuC\/DinB or Y superfamily of DNA polymerases, in stationary-phase mutagenesis and UV-induced mutagenesis of Bacillus subtilis","volume":"185","author":"Sung","year":"2003","journal-title":"J Bacteriol"},{"key":"2021092302143895600_bib45","doi-asserted-by":"crossref","first-page":"4393","DOI":"10.1093\/nar\/gkz198","article-title":"New insights into the structures and interactions of bacterial Y-family DNA polymerases","volume":"47","author":"Timinskas","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2021092302143895600_bib46","first-page":"579","article-title":"Gene expression analysis for Listeria monocytogenes following exposure to pulsed light and continuous ultraviolet light treatments","volume":"68","author":"Uesugi","year":"2016","journal-title":"Food Sci Technol"},{"key":"2021092302143895600_bib47","doi-asserted-by":"crossref","first-page":"641647","DOI":"10.1155\/2014\/641647","article-title":"Effects of growth phase and temperature on sigmaB activity within a Listeria monocytogenes population: evidence for RsbV-independent activation of sigmaB at refrigeration temperatures","volume":"2014","author":"Utratna","year":"2014","journal-title":"Biomed Res Int"},{"key":"2021092302143895600_bib48","doi-asserted-by":"crossref","first-page":"D535","DOI":"10.1093\/nar\/gkw1017","article-title":"Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center","volume":"45","author":"Wattam","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"2021092302143895600_bib49","doi-asserted-by":"crossref","first-page":"e0162832","DOI":"10.1371\/journal.pone.0162832","article-title":"The arginine\/lysine-rich element within the DNA-binding domain is essential for nuclear localization and function of the intracellular pathogen resistance 1","volume":"11","author":"Yao","year":"2016","journal-title":"PLoS One"}],"container-title":["FEMS Microbiology Letters"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femsle\/advance-article-pdf\/doi\/10.1093\/femsle\/fnab123\/40331641\/fnab123.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsle\/article-pdf\/368\/17\/fnab123\/40430031\/fnab123.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femsle\/article-pdf\/368\/17\/fnab123\/40430031\/fnab123.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T03:09:50Z","timestamp":1632366590000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsle\/article\/doi\/10.1093\/femsle\/fnab123\/6367057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":49,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2021,9,22]]}},"URL":"https:\/\/doi.org\/10.1093\/femsle\/fnab123","relation":{},"ISSN":["1574-6968"],"issn-type":[{"value":"1574-6968","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,9]]},"published":{"date-parts":[[2021,9]]},"article-number":"fnab123"}}