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It has been reported that Escherichia coli and Salmonella use the two-component system proteins QseC and QseE to recognise catecholamines and so act as bacterial adrenergic receptors. In this study, we mutated the E. coli O157:H7 and Salmonella enterica serovar Typhimurium genes encoding QseC and QseE and found that this did not block stress hormone responsiveness in either species. Motility, biofilm formation, and analysis of virulence of the mutants using two infection models were similar to the wild-type strains. The main differences in phenotypes of the qseC and qseE mutants were responses to changes in temperature and growth in different media particularly with respect to salt, carbon, and nitrogen salt sources. In this physiological respect, it was also found that the phenotypes of the qseC and qseE mutants differed between E. coli and Salmonella. These findings collectively suggest that QseC and QseE are not essential for E. coli and Salmonella to respond to stress hormones and that the proteins may be playing a role in regulating metabolism.<\/jats:p>","DOI":"10.1093\/femsle\/fnac012","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T20:12:49Z","timestamp":1644005569000},"source":"Crossref","is-referenced-by-count":8,"title":["Characterisation of the <i>E. coli<\/i> and <i>Salmonella qseC<\/i> and <i>qseE<\/i> mutants reveals a metabolic rather than adrenergic receptor role"],"prefix":"10.1093","volume":"369","author":[{"given":"Abdalla","family":"Hamed","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Medicine, University of Zawia, Zawiya QP7X+536, Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Pullinger","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Institute for Animal 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