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The current study firstly isolates Pulsatilla Radix-utilising Pediococcus pentosaceus PR-1 from human faeces. Subsequently, the tolerance of PR-1 to low pH, bile salts, simulated gastric juice and succus entericus, antioxidant activity, antimicrobial activity, cholesterol assimilation and antibiotics susceptibility were investigated. After 2\u00a0h of incubation at pH 2.0, over 80% of PR-1 survived. The cell viability of PR-1 at 2\u00a0h under 0.1% bile salt condition was 99.2%. The survival rate of PR-1 in gastric juice and succus entericus was 64.48% and 81.86%, respectively. Cell-free supernatant of PR-1 culture also showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Salmonella typhimurium. Besides, antioxidant activity of PR-1 CFS was significantly greater than cell pellet. PR-1 was shown to be resistant to kanamycin, streptomycin, vancomycin and norfloxacin and was able to lower the cholesterol level to 72.5\u00b11.5%. In addition, PR-1 displayed \u03b3-haemolysis and was non-pathogenic.<\/jats:p>","DOI":"10.1093\/femsle\/fnac089","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T09:10:02Z","timestamp":1662628202000},"source":"Crossref","is-referenced-by-count":5,"title":["Isolation and characterisation of <i>Pulsatilla Radix<\/i>-utilising bacteria <i>Pediococcus pentosaceus<\/i> PR-1 from human faeces"],"prefix":"10.1093","volume":"369","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4339-7920","authenticated-orcid":false,"given":"Yue","family":"Liu","sequence":"first","affiliation":[{"name":"Key lab of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University , Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxia","family":"Sun","sequence":"additional","affiliation":[{"name":"Key lab of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University , Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jincan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key lab of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University , Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Gao","sequence":"additional","affiliation":[{"name":"Key lab of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University , Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leilei","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Food Science and Technology, Jiangnan University , Wuxi 214122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Dong","sequence":"additional","affiliation":[{"name":"Central Laboratory, Shanxi Provincial Peoples Hospital , 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