{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:42:32Z","timestamp":1790703752570,"version":"4.1.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2600202"],"award-info":[{"award-number":["2021YFC2600202"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Development Project of Jilin Province, China","award":["YDZJ202101ZYTS124"],"award-info":[{"award-number":["YDZJ202101ZYTS124"]}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["45121031B007"],"award-info":[{"award-number":["45121031B007"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Brucella is the causative agent of brucellosis and can be transmitted to humans through aerosolized particles or contaminated food. Brucella abortus (B. abortus), Brucella melitensis (B. melitensis), and Brucella suis (B. suis) are the most virulent of the brucellae, but the traditional detection methods to distinguish them are time-consuming and require high instrumentation. To obtain epidemiological information on Brucella during livestock slaughter and food contamination, we developed a rapid and sensitive triplex recombinant polymerase amplification (triplex-RPA) assay that can simultaneously detect and differentiate between B. abortus, B. melitensis, and B. suis. Three pairs of primers (B1O7F\/B1O7R, B192F\/B192R, and B285F\/B285R) were designed and screened for the establishment of the triplex-RPA assay. After optimization, the assay can be completed within 20 min at 39\u00b0C with good specificity and no cross-reactivity with five common pathogens. The triplex-RPA assay has a DNA sensitivity of 1\u201310 pg and a minimum detection limit of 2.14\u00a0\u00d7\u00a0104\u20132.14\u00a0\u00d7\u00a0105 CFU g\u22121 in B. suis spiked samples. It is a potential tool for the detection of Brucella and can effectively differentiate between B. abortus, B. melitensis, and B. suis S2, making it a useful tool for epidemiological investigations.<\/jats:p>","DOI":"10.1093\/femsle\/fnad056","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T04:03:51Z","timestamp":1686629031000},"source":"Crossref","is-referenced-by-count":6,"title":["Arapid and sensitive triplex-recombinase polymerase amplification for simultaneous differentiation of <i>Brucella abortus, Brucella melitensis<\/i>, and <i>Brucella suis<\/i> in sera and foods"],"prefix":"10.1093","volume":"370","author":[{"given":"Jiang","family":"Chang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xusen","family":"Hou","sequence":"additional","affiliation":[{"name":"Jilin Academy of Animal Husbandry and Veterinary Medicine , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Ying","family":"Zou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"},{"name":"Panjin Center for Inspection and Testing , Panjin 124000, Liaoning Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"},{"name":"Shandong Binzhou Animal Science and Veterinary Medicine Institute , Binzhou 256600, Shandong Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Song","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Ying","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng-Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3364-8335","authenticated-orcid":false,"given":"Hong-Lin","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University , Changchun 130062, Jilin Province , P.R. 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