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Here we show that an ester hydrolase from Nocardioides strain SG-4\u00a0G has potential as a bioremediation agent against various pollutants that can be detoxified by hydrolytic cleavage of some carboxylester, carbamate, or amide linkages. Previously we showed that a radiation-killed, freeze-dried preparation (ZimA) of this strain can rapidly degrade the benzimidazole fungicide carbendazim due to the activity of a specific ester hydrolase, MheI. Here, we report that ZimA also has substantial hydrolytic activity against phthalate diesters (dimethyl, dibutyl, and dioctyl phthalate), anilide (propanil and monalide), and carbamate ester (chlorpropham) herbicides under laboratory conditions. The reaction products are substantially less toxic, or inactive as herbicides, than the parent compounds. Tests of strain SG-4\u00a0G and Escherichia coli expressing MheI found they were also able to hydrolyse dimethyl phthalate, propanil, and chlorpropham, indicating that MheI is principally responsible for the above activities.<\/jats:p>","DOI":"10.1093\/femsle\/fnad085","type":"journal-article","created":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T08:08:05Z","timestamp":1693728485000},"source":"Crossref","is-referenced-by-count":5,"title":["Wide substrate range for a candidate bioremediation enzyme isolated from <i>Nocardioides<\/i> sp. strain SG-4\u00a0G"],"prefix":"10.1093","volume":"370","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2774-7772","authenticated-orcid":false,"given":"Kishore K","family":"Krishnani","sequence":"first","affiliation":[{"name":"CSIRO Environment , Canberra, ACT 2601 , Australia"},{"name":"Central Institute of Fisheries Education , Versova, Andheri (West), Mumbai 400061 , 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