{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:56:24Z","timestamp":1753876584792,"version":"3.41.2"},"reference-count":45,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Legionella pneumophila is an opportunistic pathogen responsible for Legionnaires\u2019 disease or Legionellosis. This bacterium is found in the environment interacting with free-living amoebae such as Acanthamoeba castellanii. Until now, proteomic analyses have been done in amoebae infected with L. pneumophila but focused on the Legionella-containing vacuole. In this study, we propose a global proteomic analysis of the A. castellanii proteome following infection with L. pneumophila wild-type (WT) or with an isogenic \u0394dotA mutant strain, which is unable to replicate intracellularly. We found that infection with L. pneumophila WT leads to reduced levels of A. castellanii proteins associated with lipid homeostasis\/metabolism, GTPase regulation, and kinase. The levels of organelle-associated proteins were also decreased during infection. Legionellapneumophila WT infection leads to increased levels of proteins associated with polyubiquitination, folding or degradation, and antioxidant activities. This study reinforces our knowledge of this too little explored but so fundamental interaction between L. pneumophila and A. castellanii, to understand how the bacterium could resist amoeba digestion.<\/jats:p>","DOI":"10.1093\/femsle\/fnad086","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T03:18:31Z","timestamp":1693538311000},"source":"Crossref","is-referenced-by-count":1,"title":["Proteomic analysis of <i>Acanthamoeba castellanii<\/i> response to <i>Legionella pneumophila<\/i> infection"],"prefix":"10.1093","volume":"370","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8768-7513","authenticated-orcid":false,"given":"Alban","family":"Hay","sequence":"first","affiliation":[{"name":"Ecologie et Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers , 86073 Poitiers , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Rolland","sequence":"additional","affiliation":[{"name":"Ecologie et Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers , 86073 Poitiers , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e9ment","family":"Bernard","sequence":"additional","affiliation":[{"name":"Ecologie et Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers , 86073 Poitiers , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yann","family":"H\u00e9chard","sequence":"additional","affiliation":[{"name":"Ecologie et Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers , 86073 Poitiers , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain","family":"Vill\u00e9ger","sequence":"additional","affiliation":[{"name":"Ecologie et Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers , 86073 Poitiers , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4417-6254","authenticated-orcid":false,"given":"Ascel","family":"Samba-Louaka","sequence":"additional","affiliation":[{"name":"Ecologie et Biologie des Interactions, UMR CNRS 7267, Universit\u00e9 de Poitiers , 86073 Poitiers , France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,8,31]]},"reference":[{"key":"2023091416353982500_bib1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1039\/D0MO00087F","article-title":"Guide for protein fold change and p-value calculation for non-experts in proteomics","volume":"16","author":"Aguilan","year":"2020","journal-title":"Mol Omics"},{"key":"2023091416353982500_bib2","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1128\/IAI.00956-06","article-title":"Environmental mimics and the Lvh type IVA secretion system contribute to virulence-related phenotypes of 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