{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:47:38Z","timestamp":1787827658841,"version":"build-2784847793"},"reference-count":36,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Traditional diagnosis of infectious gastroenteritis is based on culture, microscopy and antigen detection. The development of gastrointestinal syndromic panels based on molecular techniques have allowed rapid and simultaneous identification of multiple pathogens. The objective was to evaluate the implementation of Allplex\u2122 Gastrointestinal Panel Assays (AGPA): Allplex\u2122 GI-Virus, Allplex\u2122 GI-Bacteria (I) and Allplex\u2122 GI-Parasite by comparing with traditional diagnosis. A retrospective comparative study was conducted at Hospital Universitario La Paz, between the first year of implementation of the AGPA (April 1, 2018 to March 31, 2019) and the results obtained during the previous year with traditional methods (April 1, 2017 to March 31, 2018). With the implementation of AGPA we obtained an increase in the detection of rotavirus and adenovirus, being statistically significant for rotavirus ([CI95%:3.60\u20136.79]; P\u00a0&amp;lt;\u00a00.05) and an increase in the positivity rates of all the bacteria tested, with the exception of Salmonella spp. ([CI95%:3.60\u20136.79]; P\u00a0&amp;lt;\u00a00.05). Comparing the bacteria recovered by culture, we obtained an increase in the case of Shigella spp. cultivation during the AGPA period. Regarding protozoa, we achieved a significant increase in the positivity rates for Cryptosporidium spp. ([CI95%:1.98\u20133.01] P\u00a0&amp;lt;\u00a00.05), Giardia intestinalis ([CI95%:3.94\u20135.25]; P\u00a0&amp;lt;\u00a00.05) and Blastocystis spp. ([CI95%:9.44\u201311.36]; P\u00a0&amp;lt;\u00a00.05). There was an improvement in report turnaround time when comparing molecular diagnosis to bacterial culture and concentration plus microscopy for parasites; but not compared with antigen detection. The molecular diagnosis approach with AGPA were more sensitive and had a faster turnaround time for some targets, and in our setting, enabled an increased diagnostic capacity for viruses and protozoa.<\/jats:p>","DOI":"10.1093\/femsle\/fnad122","type":"journal-article","created":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T07:47:36Z","timestamp":1699948056000},"source":"Crossref","is-referenced-by-count":9,"title":["Diagnosis of gastrointestinal infections: comparison between traditional microbiology and a commercial syndromic molecular-based panel"],"prefix":"10.1093","volume":"370","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4039-2638","authenticated-orcid":false,"given":"Jorge","family":"Ligero-L\u00f3pez","sequence":"first","affiliation":[{"name":"Clinical Microbiology and Parasitology Department, Hospital Universitario La Paz , 28046 - Madrid , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Garc\u00eda-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Parasitology Department, Hospital Universitario La Paz , 28046 - Madrid , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Ruiz-Carrascoso","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Parasitology Department, Hospital Universitario La Paz , 28046 - Madrid , Spain"},{"name":"CIBERINFEC, Instituto de Salud Carlos III , 28029 - Madrid , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,13]]},"reference":[{"key":"2023112502563667700_bib1","doi-asserted-by":"crossref","first-page":"8135724","DOI":"10.1155\/2020\/8135724","article-title":"An overview of the molecular methods in the diagnosis of gastrointestinal infectious diseases","volume":"2020","author":"Amjad","year":"2020","journal-title":"Int J Microbiol"},{"key":"2023112502563667700_bib2","first-page":"e01457","article-title":"A gastrointestinal PCR panel improves clinical management and lowers health care costs","volume":"56","author":"Beal","year":"2017","journal-title":"J Clin Microbiol"},{"key":"2023112502563667700_bib3","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1128\/JCM.01489-10","article-title":"New methods for detection of campylobacters in stool samples in comparison to culture","volume":"49","author":"Bess\u00e8de","year":"2011","journal-title":"J Clin Microbiol"},{"key":"2023112502563667700_bib4","doi-asserted-by":"crossref","first-page":"711809","DOI":"10.3389\/fmed.2021.711809","article-title":"Systematic review on the correlation of quantitative PCR cycle threshold values of gastrointestinal pathogens with patient clinical presentation and outcomes","volume":"8","author":"Bonacorsi","year":"2021","journal-title":"Front Med (Lausanne)"},{"key":"2023112502563667700_bib5","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1128\/JCM.02674-14","article-title":"Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis","volume":"53","author":"Buss","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2023112502563667700_bib6","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.anpedi.2019.01.024","article-title":"\u00bfQu\u00e9 hemos aprendido sobre rotavirus en Espa\u00f1a en los \u00faltimos 10 a\u00f1os? 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