{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:17:58Z","timestamp":1791321478134,"version":"4.3.1"},"reference-count":41,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human-induced pluripotent stem cell-derived small intestinal epithelial cell (hiPSC-SIEC) monolayers are useful in vitro models for evaluating the gut mucosal barrier; however, their reactivity to cytokines, which are closely related to the regulation of mucosal barrier function, remains unclear. Interleukin (IL)-22 is a cytokine that contributes to regulate the mucosal barrier in the intestinal epithelia. Using microarray and gene set enrichment analysis, we found that hiPSC-SIEC monolayers activate the immune response and enhance the mucosal barrier in response to IL-22. Moreover, hiPSC-SIEC monolayers induced the gene expression of antimicrobials, including the regenerating islet-derived protein 3 family. Furthermore, IL-22 stimulation upregulated Mucin 2 secretion and gene expression of an enzyme that modifies sugar chains, suggesting alteration of the state of the mucus layer of hiPSC-SIEC monolayers. To evaluate its physiological significance, we measured the protective activity against Salmonella enterica subsp. enterica infection in hiPSC-SIEC monolayers and found that prestimulation with IL-22 reduced the number of viable intracellular bacteria. Collectively, these results suggest that hiPSC-SIEC monolayers enhance the mucosal barrier and inhibit infection by pathogenic bacteria in response to IL-22, as previously reported. These results can contribute to the further application of hiPSC-SIECs in evaluating mucosal barriers.<\/jats:p>","DOI":"10.1093\/femsle\/fnae006","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T23:25:14Z","timestamp":1705965914000},"source":"Crossref","is-referenced-by-count":6,"title":["Interleukin-22 enhanced the mucosal barrier and inhibited the invasion of\n                    <i>Salmonella enterica<\/i>\n                    in human-induced pluripotent stem cell-derived small intestinal epithelial cells"],"prefix":"10.1093","volume":"371","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5731-0614","authenticated-orcid":false,"given":"Fuka","family":"Yamazaki","sequence":"first","affiliation":[{"name":"Food Microbiology and Function Research Laboratories, R&D Division, Meiji Co., Ltd , 1-29-1 Nanakuni, Hachioji, Tokyo 192\u20130919 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8962-0649","authenticated-orcid":false,"given":"Kyosuke","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Food Microbiology and Function Research Laboratories, R&D Division, Meiji Co., Ltd , 1-29-1 Nanakuni, Hachioji, Tokyo 192\u20130919 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Food Microbiology and Function Research Laboratories, R&D Division, Meiji Co., Ltd , 1-29-1 Nanakuni, Hachioji, Tokyo 192\u20130919 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7617-0987","authenticated-orcid":false,"given":"Toshihiro","family":"Sashihara","sequence":"additional","affiliation":[{"name":"Food Microbiology and Function Research Laboratories, R&D Division, Meiji Co., Ltd , 1-29-1 Nanakuni, Hachioji, Tokyo 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