{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:57:45Z","timestamp":1753876665356,"version":"3.41.2"},"reference-count":46,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","award":["NRF-2016R1D1A3B01008304"],"award-info":[{"award-number":["NRF-2016R1D1A3B01008304"]}],"id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study was designed to evaluate the history-dependent behaviors of Salmonella Typhimurium re-exposed to sublethal levels of ciprofloxacin. The S. Typhimurium cells were pre-exposed to 0 (CON), 1\/16 (LOW), 1\/8 (MED), and 1\/4 (HIGH) minimum inhibitory concentrations (MICs) of ciprofloxacin, followed by re-exposure to the same concentrations. The bacterial growth, postantibiotic effect (PAE), relative fitness, and swimming motility of treatments were evaluated in the absence of ciprofloxacin. The lag phase duration (LPD) was estimate to assess bacterial recovery under ciprofloxacin exposure. A disk diffusion assay was used to determine the cross-resistance and collateral sensitivity of CON, LOW, MED, and HIGH treatments to ciprofloxacin (CIP), ceftriaxone (CEF), erythromycin (ERY), gentamicin (GEN), and polymyxin B (POL). The S. Typhimurium cells pre-exposed to ciprofloxacin were susceptible in antibiotic-free media, showing delayed growth. The highest PAE (&amp;gt;1\u00a0h) and bacterial fluctuation (CV\u00a0=\u00a05%) were observed at the High treatment compared to the CON. The HIGH treatment had the lowest relative fitness levels (0.87) and swimming motility (55\u00a0mm). The LPD was significantly decreased at the LOW treatment (1.8\u00a0h) when re-exposed to 1\/16\u00a0\u00d7\u00a0MIC of ciprofloxacin. The LOW, MED, and HIGH treatments showed the cross-resistance to POL and the collateral sensitivity to CEF, ERY, and GEN. The pre-exposure to ciprofloxacin could induce phenotypic diversity, corresponding to the history-dependent behaviors. These results provide important insights for the dynamic nature of bacterial populations when re-exposed to sublethal concentrations of antibiotics.<\/jats:p>","DOI":"10.1093\/femsle\/fnae050","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T13:52:56Z","timestamp":1719409976000},"source":"Crossref","is-referenced-by-count":0,"title":["Dynamic responses of <i>Salmonella<\/i> Typhimurium to re-exposure to sublethal ciprofloxacin"],"prefix":"10.1093","volume":"371","author":[{"given":"Jiseok","family":"Yi","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, Kangwon National University , Chuncheon, Gangwon 24341 , Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Kangwon National University , Chuncheon, Gangwon 24341 , Republic of 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