{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:49:31Z","timestamp":1753876171722,"version":"3.41.2"},"reference-count":33,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001691","name":"KAKENHI","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Grant-in-Aid for Scientific Research","award":["26460528"],"award-info":[{"award-number":["26460528"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Streptococcus intermedius secretes the human-specific cytolysin intermedilysin (ILY), a crucial factor in the pathogenicity of this bacterium. Previously, we reported that a lactose phosphotransferase repressor (LacR) represses ily expression, and that its mutation increases ILY production. Interestingly, UNS40, a strain isolated from a liver abscess, produces high levels of ILY despite the absence of mutations in the lacR promoter and coding regions. Our results showed that a G\u00a0&amp;gt;\u00a0A mutation at the\u00a0\u221290th position from the transcription start point in the UNS40 ily promoter region increased hemolytic activity and decreased the binding ability to LacR. To elucidate the regions involved in the repression of ily expression, we generated mutant strains, in which point or deletion mutations were introduced into the ily promoter region, and then compared their hemolytic activity. Among the point mutations, \u2212120 C\u00a0&amp;gt;\u00a0A and\u00a0\u221290 G\u00a0&amp;gt;\u00a0A and their flanking mutations increased hemolytic activity. These results indicated that these mutations may increase the virulence of S. intermedius.<\/jats:p>","DOI":"10.1093\/femsle\/fnae063","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T06:45:26Z","timestamp":1722926726000},"source":"Crossref","is-referenced-by-count":0,"title":["Identification of mutations resulting in derepression of the intermedilysin gene by sequential mutagenesis of its promoter region in <i>Streptococcus intermedius<\/i>"],"prefix":"10.1093","volume":"371","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3724-3747","authenticated-orcid":false,"given":"Toshifumi","family":"Tomoyasu","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Division of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University Graduate School , 2-1 Minami-Jousanjima-Cho , Tokushima, Tokushima 770\u20138513, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4896-1738","authenticated-orcid":false,"given":"Atsushi","family":"Tabata","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Division of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University Graduate School , 2-1 Minami-Jousanjima-Cho , Tokushima, Tokushima 770\u20138513, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9995-7635","authenticated-orcid":false,"given":"Hideaki","family":"Nagamune","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Division of Bioscience and Bioindustry, Graduate School of Technology, Industrial and Social Sciences, Tokushima University Graduate School , 2-1 Minami-Jousanjima-Cho , Tokushima, Tokushima 770\u20138513, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,8,5]]},"reference":[{"key":"2024082709012812900_bib1","doi-asserted-by":"crossref","first-page":"3752","DOI":"10.1128\/JB.187.11.3752-3761.2005","article-title":"Fructose utilization in Lactococcus lactis as a model for low-GC Gram-positive bacteria: its regulator, signal, and DNA-binding site","volume":"187","author":"Barri\u00e8re","year":"2005","journal-title":"J Bacteriol"},{"key":"2024082709012812900_bib2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1574-6968.2000.tb08895.x","article-title":"Thiol-activated cytolysins: structure, function and role in pathogenesis","volume":"182","author":"Billington","year":"2000","journal-title":"FEMS Microbiol Lett"},{"key":"2024082709012812900_bib3","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1038\/nsmb862","article-title":"Human CD59 is a receptor for the cholesterol-dependent cytolysin intermedilysin","volume":"11","author":"Giddings","year":"2004","journal-title":"Nat Struct Mol Biol"},{"key":"2024082709012812900_bib4","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00886-16","article-title":"Characterization of the pathogenicity of Streptococcus intermedius TYG1620 isolated from a human brain abscess based on the complete genome sequence with transcriptome analysis and transposon mutagenesis in a murine subcutaneous abscess model","volume":"85","author":"Hasegawa","year":"2017","journal-title":"Infect Immun"},{"key":"2024082709012812900_bib5","doi-asserted-by":"crossref","first-page":"3642","DOI":"10.1128\/JB.00155-11","article-title":"Characterization of DNA binding sites of the ComE response regulator from Streptococcus mutans","volume":"193","author":"Hung","year":"2011","journal-title":"J Bacteriol"},{"key":"2024082709012812900_bib6","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3390\/pathogens8010022","article-title":"Genome mining and comparative analysis of Streptococcus intermedius causing brain abscess in a child","volume":"8","author":"Issa","year":"2019","journal-title":"Pathogens"},{"key":"2024082709012812900_bib7","doi-asserted-by":"crossref","first-page":"826","DOI":"10.3389\/fmicb.2020.00826","article-title":"From normal flora to brain abscesses: a review of Streptococcus intermedius","volume":"11","author":"Issa","year":"2020","journal-title":"Front Microbiol"},{"key":"2024082709012812900_bib8","doi-asserted-by":"crossref","first-page":"e56418","DOI":"10.1371\/journal.pone.0056418","article-title":"Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis","volume":"8","author":"Jensen","year":"2013","journal-title":"PLoS One"},{"key":"2024082709012812900_bib9","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1099\/ijs.0.043232-0","article-title":"Taxonomy of the Anginosus group of the genus Streptococcus and description of Streptococcus anginosus subsp. 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