{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T17:56:41Z","timestamp":1786816601786,"version":"build-2736575974"},"reference-count":38,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2303900"],"award-info":[{"award-number":["2022YFC2303900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In recent years, the emergence of multidrug-resistant bacteria has limited the selection of drugs for treating bacterial infections, reduced clinical efficacy, and increased treatment costs and mortality. It is urgent to find alternative antibiotics. In order to explore a new method for controlling methicillin-resistant Staphylococcus aureus (S. aureus), this study isolated and purified a multidrug-resistant S. aureus broad-spectrum phage JPL-50 from wastewater. JPL-50 belongs to the Siphoviridae family after morphological observation, biological characterization, and transmission electron microscopy (TEM) fragmentation spectrum analysis. It can cleave 84% of tested S. aureus (168\/200), in which 100% of tested mastitis-associated strains (48\/48) and 72.04% of MRSA strains (67\/93) were lysed. In addition, it has an optimal growth temperature of about 30\u00b0C, a high activity within a wide pH range (pH 3\u201310), and an optimal multiplicity of infection of 0.01. The one-step growth curve shows a latent time of 20 min, an explosive time of 80 min. JPL-50 was 16\u2009927\u00a0bp in length and was encoded by double-stranded DNA, with no genes associated with bacterial resistance or virulence factors detected. In a therapeutic study, injection of the phage JPL-50 once and for 7 times in 7 days protected 40% and 60% of the mice from fatal S. aureus infection, respectively. More importantly, JPL-50-doxycycline combination could effectively inhibit host S. aureus in vitro and reduce the use of doxycycline within 8 h. In conclusion, the bacteriophage JPL-50 has a wide lysis spectrum, high lysis rate, high tolerance to extreme environments, and moderate in vivo activity, providing ideas for developing multidrug-resistant S. aureus infections.<\/jats:p>","DOI":"10.1093\/femsle\/fnae072","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T05:59:12Z","timestamp":1726207152000},"source":"Crossref","is-referenced-by-count":3,"title":["Isolation and characterization of a multidrug-resistant <i>Staphylococcus aureus<\/i> infecting phage and its therapeutic use in mice"],"prefix":"10.1093","volume":"371","author":[{"given":"Zhen","family":"Xiao","sequence":"first","affiliation":[{"name":"China animal health and epidemiology center , Qingdao 266032 ,","place":["China"]},{"name":"College of Veterinary Medicine, Qingdao Agricultural University , Qingdao 266109 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