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Differentiation and genotyping were established via biochemical profiles and polymerase chain reaction. Illumina MiSeq\u00ae was applied to sequence the Brucella isolates. Using multilocus sequence typing and Fisher\u2019s exact test was used for the KEGG enrichment analysis of differential genes. Differential gene protein-protein interaction (PPI) network analysis was conducted using the STRING database and visualization was performed using. According to the final identification results of an A\/M serum agglutination test. ARQ-070 was identified as the No. 3 white spirulina biological variety, and mM was identified as the No. 1 white Spirulina biological variety. The genetic information of this strain is very close to that of the M5 vaccine strain, suggesting possible vaccine-escape infection. In the comparative genomics analysis with B. melitensis bv.1 16\u00a0M, the main differences between the B. melitensis strains were found to be concentrated in the genes related to amino acid metabolism and environmental perception. A base mutation was found in the Brucella gene virB9, which is associated with the key virulence factor of the type IV secretion system, but this mutation did not lead to changes in the protein\u2019s tertiary structure, and the strain did not lose its infectivity. The study discovered a base mutation in the virB9 gene of Brucella, which is linked to T4SS but does not affect the protein\u2019s structure or the strain\u2019s infectivity. This mutation could influence public health approaches to detecting and preventing Brucella transmission. Future research aims to analyse a wider range of Brucella strains for a deeper understanding of their epidemiology.<\/jats:p>","DOI":"10.1093\/femsle\/fnae085","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T01:26:32Z","timestamp":1731547592000},"source":"Crossref","is-referenced-by-count":2,"title":["Origin tracking of <i>Brucella<\/i> strain <i>B. melitensics bv.3<\/i> ARQ-070 using biochemical and genomic studies"],"prefix":"10.1093","volume":"371","author":[{"given":"Na","family":"Ta","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Inner Mongolia Agricultural University , Hohhot 010010 ,","place":["China"]},{"name":"Inner Mongolia Center for Disease Control and Research , Hohhot 010051 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke-Ming","family":"Zuo","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Inner Mongolia Agricultural University , Hohhot 010010 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Public Health, Baotou Medical College , Baotou City 014040 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Inner Mongolia Agricultural University , Hohhot 010010 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Tao","family":"Song","sequence":"additional","affiliation":[{"name":"Inner Mongolia Center for Disease Control and Research , Hohhot 010051 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Jun","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Inner Mongolia Agricultural University , Hohhot 010010 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Ping","family":"Yu","sequence":"additional","affiliation":[{"name":"Inner Mongolia Center for Disease Control and Research , Hohhot 010051 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,11,13]]},"reference":[{"key":"2024121420301113600_bib1","doi-asserted-by":"crossref","first-page":"e0031122","DOI":"10.1128\/jcm.00311-22","article-title":"Core genome multilocus sequence typing scheme for improved characterization and epidemiological surveillance of pathogenic brucella","volume":"60","author":"Abdel-Glil","year":"2022","journal-title":"J Clin Microbiol"},{"key":"2024121420301113600_bib2","doi-asserted-by":"crossref","first-page":"771510","DOI":"10.3389\/fcimb.2021.771510","article-title":"Antibiotic resistance: one health one world outlook","volume":"11","author":"Aslam","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"2024121420301113600_bib3","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.femsle.2005.09.030","article-title":"From bioremediation to biowarfare: on the impact and mechanism of type IV secretion systems","volume":"253","author":"Baron","year":"2005","journal-title":"FEMS Microbiol Lett"},{"key":"2024121420301113600_bib4","doi-asserted-by":"crossref","first-page":"109249","DOI":"10.1016\/j.vetmic.2021.109249","article-title":"A core genome multilocus sequence typing scheme for Mycoplasma hyorhinis","volume":"262","author":"B\u00fcnger","year":"2021","journal-title":"Vet Microbiol"},{"key":"2024121420301113600_bib5","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1146\/annurev-animal-021815-111326","article-title":"Brucella spp. Virulence factors and immunity","volume":"4","author":"Byndloss","year":"2016","journal-title":"Annu Rev Anim Biosci"},{"key":"2024121420301113600_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1097(03)00963-7","article-title":"Brucella pathogenesis, genes identified from random large-scale screens","volume":"231","author":"Delrue","year":"2004","journal-title":"FEMS Microbiol Lett"},{"key":"2024121420301113600_bib7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0378-1135(02)00252-3","article-title":"Epidemiology and control of brucellosis in China","volume":"90","author":"Deqiu","year":"2002","journal-title":"Vet Microbiol"},{"key":"2024121420301113600_bib8","first-page":"497","article-title":"Tests of immunologic competence. 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