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In this study, we performed whole-genome sequencing and bioinformatic analyses on eight multidrug-resistant Escherichia coli isolates previously obtained from domestic WWTPs in Costa Rica. We identified 61 ARGs (23 unique), with 40 located on plasmids, and 21 on chromosomal sequences, seven of which were within integrons. Several ARGs were associated with resistance to clinically and veterinary important antibiotics, including sulfamethoxazole\/trimethoprim, beta-lactams, and tetracyclines. One hundred twenty-one virulence-associated genes (29 unique) were detected, with 16 located on plasmids. Notably, the presence of virulence factors such as ompT and hlyF genes alongside ARGs on plasmids underscores the transmissible pathogenic potential of WWTP-associated E. coli strains. These findings highlight the role of small domestic WWTPs in disseminating pathogenic and multidrug-resistant bacteria and their mobile genetic elements, emphasizing the need for further research to understand how these discharges impact aquatic environments.<\/jats:p>","DOI":"10.1093\/femsle\/fnaf041","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T07:33:13Z","timestamp":1744702393000},"source":"Crossref","is-referenced-by-count":5,"title":["Plasmid and integron-associated antibiotic resistance in <i>Escherichia coli<\/i> isolated from domestic wastewater treatment plants"],"prefix":"10.1093","volume":"372","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3214-4599","authenticated-orcid":false,"given":"Bradd","family":"Mendoza-Guido","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Salud (INISA), Universidad de Costa Rica , San Jos\u00e9 11501 ,","place":["Costa Rica"]},{"name":"Programa de Doctorado en Ciencias, 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