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Here, we report that while nine tested P. aeruginosa strains were indeed triclosan-resistant when grown on Luria Bertani agar supplemented with triclosan, all nine exhibited decreased CFU by plating following pre-exposure to phosphate-buffer saline (PBS). Compared to growth on Luria Bertani agar in the absence of PBS incubation, PBS pre-exposure was associated with mean CFU reductions ranging from \u223c25% (clinical isolate PABL048) to \u223c80% (laboratory strain PA14). The potential for triclosan agar to cause significant errors in experiments designed to quantify P. aeruginosa numbers was demonstrated using a mouse model of P. aeruginosa gastrointestinal carriage. In this experiment, P. aeruginosa carriage was underestimated by up to 20-fold when fecal samples were processed using PBS. These findings suggest that triclosan should be used with caution to quantify P. aeruginosa numbers in protocols that utilize PBS.<\/jats:p>","DOI":"10.1093\/femsle\/fnag086","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:40:52Z","timestamp":1784893252000},"source":"Crossref","is-referenced-by-count":0,"title":["Pre-exposure to phosphate-buffer saline (PBS) renders some\n                    <i>Pseudomonas aeruginosa<\/i>\n                    strains susceptible to triclosan on solid medium"],"prefix":"10.1093","volume":"373","author":[{"given":"Marine","family":"Lebrun-Corbin","sequence":"first","affiliation":[{"name":"Feinberg School of Medicine, Northwestern University Department of Microbiology-Immunology, , Chicago, IL 60611 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somalisa","family":"Pan","sequence":"additional","affiliation":[{"name":"Feinberg School of Medicine, Northwestern University Department of Microbiology-Immunology, , Chicago, IL 60611 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preeti","family":"Garai","sequence":"additional","affiliation":[{"name":"Feinberg School of Medicine, Northwestern University Department of Microbiology-Immunology, , Chicago, IL 60611 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4596-7939","authenticated-orcid":false,"given":"Alan R","family":"Hauser","sequence":"additional","affiliation":[{"name":"Feinberg School of Medicine, Northwestern University Department of Microbiology-Immunology, , Chicago, IL 60611 ,","place":["United States"]},{"name":"Division of Infectious Diseases, Feinberg School of Medicine, Northwestern University Department of Medicine, , Chicago, IL 60611 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"2026080417512039100_bib1","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1038\/s41467-020-14363-4","article-title":"Systemic infection facilitates transmission of Pseudomonas aeruginosa in mice","volume":"11","author":"Bachta","year":"2020","journal-title":"Nat Commun"},{"key":"2026080417512039100_bib2","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1067\/mic.2003.11","article-title":"High-level triclosan resistance in Pseudomonas aeruginosa is solely a result of efflux","volume":"31","author":"Chuanchuen","year":"2003","journal-title":"Am J Infect Control"},{"key":"2026080417512039100_bib3","article-title":"COPAN","author":"ESwab\u00ae Liquid Based Collection and Transport","year":"2024"},{"key":"2026080417512039100_bib4","doi-asserted-by":"publisher","first-page":"S775","DOI":"10.1093\/clinids\/6.Supplement_3.S775","article-title":"Classics in infectious diseases. 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