{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T15:10:10Z","timestamp":1785510610545,"version":"3.56.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":205,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide, and resistance to major antibiotic classes is increasing. Exploiting collateral sensitivity (CS) may be an effective approach to optimize antibiotic use and mitigate the impact of resistance. In this study, we investigated CS and collateral resistance (CR) profiles in experimentally evolved C. jejuni mutants derived from ATCC 29428 resistant to ciprofloxacin, erythromycin, gentamicin, and tetracycline. Minimum inhibitory concentrations of thirteen antibiotics were determined in the wild-type and mutant strains to characterize collateral effects. Whole-genome sequencing was performed to identify mutations potentially underlying the observed collateral effects, and bacterial growth curves were used to evaluate the fitness of the mutants. Ciprofloxacin-resistant mutants exhibited CS most consistently to aminoglycosides, potentially explained by mutations in gyrA, parC, and parE. Gentamicin-resistant mutants showed CS to macrolides, fosfomycin, and amoxicillin. Erythromycin-resistant mutants displayed CS to aminoglycosides, albeit inconsistently across biological replicates. No CS was observed in tetracycline-resistant mutants, which showed CR to other ribosome-targeting antibiotics, potentially due to mutations in rpsJ. Consistent CR was observed among antibiotics of the same class. These findings demonstrate CS and CR patterns in C. jejuni, highlighting evolutionary trade-offs that could inform more effective treatment strategies against resistant C. jejuni infections.<\/jats:p>","DOI":"10.1093\/femsle\/fnag089","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:40:53Z","timestamp":1784893253000},"source":"Crossref","is-referenced-by-count":0,"title":["Collateral effects of antibiotic resistance in\n                    <i>Campylobacter jejuni<\/i>"],"prefix":"10.1093","volume":"373","author":[{"given":"Hadiseh","family":"Zandrajabi","sequence":"first","affiliation":[{"name":"Division of Systems Pharmacology and Pharmacy, Leiden Academic Center for Drug Research, Leiden University , 2333 CC Einsteinweg 55 ,","place":["The Netherlands"]},{"name":"Faculty of Agriculture, Ferdowsi University of Mashhad Department of Food Science and Technology, , Azadi Square, 9177948974 Mashhad ,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wisse","family":"van\u00a0Os","sequence":"additional","affiliation":[{"name":"Division of Systems Pharmacology and Pharmacy, Leiden Academic Center for Drug Research, Leiden University , 2333 CC Einsteinweg 55 ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7409-2847","authenticated-orcid":false,"given":"Wiep Klaas","family":"Smits","sequence":"additional","affiliation":[{"name":"Experimental Bacteriology, Leiden University Medical Center, Albinusdreef 2 , 2333 ZA Leiden ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Ali","family":"Mortazavi","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Ferdowsi University of Mashhad Department of Food Science and Technology, , Azadi Square, 9177948974 Mashhad ,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farideh","family":"Tabatabaei-Yazdi","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Ferdowsi University of Mashhad Department of Food Science and Technology, , Azadi Square, 9177948974 Mashhad ,","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1664-7314","authenticated-orcid":false,"given":"Johan G C","family":"van\u00a0Hasselt","sequence":"additional","affiliation":[{"name":"Division of Systems Pharmacology and Pharmacy, Leiden Academic Center for Drug Research, Leiden University , 2333 CC Einsteinweg 55 ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"2026073110365875100_bib1","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1111\/j.1574-6968.2007.00935.x","article-title":"Antibiotic 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