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Here, we assembled and investigated the circular 575\u00a0kb genome of Candidatus Argiopiplasma dusa, a novel bacterial symbiont of the wasp spider Argiope bruennichi. Phylogenomic analysis placed this species within the phylum Mycoplasmatota, in a poorly characterized clade that may represent a new order-level lineage or affiliate with the Mycoplasmatales order. With 559 predicted genes, the genome is relatively small compared to other Mycoplasmatota genomes (on average 969 genes) and has a low GC content of \u223c24%. While the genome encodes genes for proteins involved in glycolysis and fermentative acetate production, it revealed minimal biosynthetic capabilities with pathways for nucleotide, amino acid, and vitamin biosynthesis being absent in Ca. Argiopiplasma dusa. This suggests a metabolic dependence and an endosymbiotic lifestyle within the spider host. The symbiont was detected in A. bruennichi populations across the distribution range of the spider but appears to be absent in certain populations.<\/jats:p>","DOI":"10.1093\/femsle\/fnag103","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:17:32Z","timestamp":1789989452000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic insights into\n                    <i>Candidatus<\/i>\n                    Argiopiplasma dusa, a bacterial symbiont of the wasp spider\n                    <i>Argiope bruennichi<\/i>"],"prefix":"10.1093","volume":"373","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9545-6355","authenticated-orcid":false,"given":"Larissa M","family":"Busch","sequence":"first","affiliation":[{"name":"Center for Functional Genomics of Microbes, University of Greifswald , 17489 Greifswald ,","place":["Germany"]},{"name":"Institute of Mathematics and Computer Science, University of Greifswald , 17489Greifswald ,","place":["Germany"]},{"name":"Institute of Microbiology, University of Greifswald , 17489 Greifswald ,","place":["Germany"]},{"name":"Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald Department of Functional Genomics, , 17489 Greifswald ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6527-4198","authenticated-orcid":false,"given":"Monica M","family":"Sheffer","sequence":"additional","affiliation":[{"name":"Zoological Institute and Museum, University of Greifswald , 17489 Greifswald ,","place":["Germany"]},{"name":"California Polytechnic University Department of Biological Sciences, , Humboldt, Arcata, CA 95521 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1917-2577","authenticated-orcid":false,"given":"Nina","family":"Dombrowski","sequence":"additional","affiliation":[{"name":"Royal Netherlands Institute for Sea Research (NIOZ) Department of Marine Microbiology and Biogeochemistry, , NL-1790 AB Den Burg ,","place":["The Netherlands"]},{"name":"Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam Department of Evolutionary & Population Biology, , 1090 GE Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5069-8601","authenticated-orcid":false,"given":"Henrik","family":"Krehenwinkel","sequence":"additional","affiliation":[{"name":"University of Trier Department of Biogeography, , 54296 Trier ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6229-3596","authenticated-orcid":false,"given":"Stefan","family":"Prost","sequence":"additional","affiliation":[{"name":"Ecology and Genetics Research Unit, University of Oulu , 90014 Oulu ,","place":["Finland"]},{"name":"Central Research Laboratories, Natural History Museum Vienna , 1010 Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6518-8556","authenticated-orcid":false,"given":"Anja","family":"Spang","sequence":"additional","affiliation":[{"name":"Royal Netherlands Institute for Sea Research (NIOZ) Department of Marine Microbiology and Biogeochemistry, , NL-1790 AB Den Burg ,","place":["The Netherlands"]},{"name":"Institute for Biodiversity and Ecosystem Dynamics (IBED), University of Amsterdam Department of Evolutionary & Population Biology, , 1090 GE Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8758-7913","authenticated-orcid":false,"given":"Gabriele","family":"Uhl","sequence":"additional","affiliation":[{"name":"Zoological Institute and Museum, University of Greifswald , 17489 Greifswald 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