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Borrelia burgdorferi has a linear chromosome with a number of circular and linear plasmids. Bacteria, including B. burgdorferi, release spherical outer membrane vesicles (OMVs) that are known to carry secretory products including metabolites, nucleic acids and proteins. Herein, we provide the first comparative transcriptomic analysis of the vesicles released from B. burgdorferi. We identified a total of \u223c1200 unique transcripts with at least one mapped read from the bacterial cell and its OMVs. We compared the spectrum of transcripts between bacterial cell and its OMVs, and found a biased distribution based on the source of transcripts, i.e. plasmid-encoded transcripts are more likely to be enriched in the OMVs. We validated the distribution for some of the transcripts by qPCR. This analysis provides the first evidence that some of the B. burgdorferi transcripts are preferentially packaged in OMV, which further suggest that the bacteria might use its OMVs for bacteria\u2013bacteria or bacteria\u2013host communications. This report also suggests a possible involvement of Borrelia-derived OMVs in the development of Lyme disease in both early and post disease syndromes.<\/jats:p>","DOI":"10.1093\/femsle\/fny135","type":"journal-article","created":{"date-parts":[[2018,5,27]],"date-time":"2018-05-27T03:06:51Z","timestamp":1527390411000},"source":"Crossref","is-referenced-by-count":24,"title":["mRNA transcript distribution bias between <i>Borrelia burgdorferi<\/i> bacteria and their outer membrane vesicles"],"prefix":"10.1093","volume":"365","author":[{"given":"Anjali","family":"Malge","sequence":"first","affiliation":[{"name":"Institute for Systems Biology, 401 Terry Avenue North Seattle, WA 98109-5263, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1968-7060","authenticated-orcid":false,"given":"Vikas","family":"Ghai","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology, 401 Terry Avenue North Seattle, WA 98109-5263, 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