{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:59:29Z","timestamp":1753876769613,"version":"3.41.2"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T00:00:00Z","timestamp":1555027200000},"content-version":"vor","delay-in-days":11,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["2012\u201367\u00a0017-30\u00a0218"],"award-info":[{"award-number":["2012\u201367\u00a0017-30\u00a0218"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2012\u201367\u00a0017-30\u00a0218"],"award-info":[{"award-number":["2012\u201367\u00a0017-30\u00a0218"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Several outbreaks of listeriosis have implicated fresh produce but genetic factors required for growth of Listeria monocytogenes on produce remain poorly characterized. Based on the fact that \u03b2-lactam antibiotics only kill bacterial cells that are growing, we hypothesized that ampicillin selection can enrich for L. monocytogenes mutants unable to grow on produce. For validation, we examined relative recovery of L. monocytogenes strain 2011L-2858 and its cold-sensitive mutant L1E4 following inoculation of cantaloupe rind fragments with 1:1 mixture of the strains and incubation at 4\u00b0C with or without ampicillin. Listeria monocytogenes from rind fragments inoculated with the mixed cultures and incubated in the presence of ampicillin were used to inoculate fresh rind fragments for a second round of enrichment. In the presence of ampicillin, the proportion of L1E4 increased from 55% on day 0 to 78% on day 14, with higher recovery (85% after 14 days) in the second round of enrichment. These data suggested that L1E4 was enriched on cantaloupe rind fragments while growing cells of the wildtype were killed by ampicillin. Application of this protocol to transposon mutant libraries from three L. monocytogenes strains yielded several mutants unable to grow on cantaloupe. Thus, ampicillin selection can facilitate discovery of genes essential for growth of L. monocytogenes on fresh produce.<\/jats:p>","DOI":"10.1093\/femsle\/fnz076","type":"journal-article","created":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T01:04:49Z","timestamp":1554944689000},"source":"Crossref","is-referenced-by-count":2,"title":["Validation of an ampicillin selection protocol to enrich for mutants of <i>Listeria monocytogenes<\/i> unable to replicate on fresh produce"],"prefix":"10.1093","volume":"366","author":[{"given":"Victor","family":"Jayeola","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5307-2222","authenticated-orcid":false,"given":"C","family":"Parsons","sequence":"additional","affiliation":[{"name":"Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Gorski","sequence":"additional","affiliation":[{"name":"Agricultural Research Service, United States Department of Agriculture, Albany, CA 94710, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Kathariou","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA"},{"name":"Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,4,12]]},"reference":[{"key":"2022030901054618300_bib1","doi-asserted-by":"crossref","first-page":"6855","DOI":"10.1128\/AEM.01006-13","article-title":"Characterization of a potential Listeria monocytogenes virulence factor associated with attachment to fresh produce","volume":"79","author":"Bae","year":"2013","journal-title":"Appl Environ Microbiol"},{"key":"2022030901054618300_bib2","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1038\/nrmicro1743","article-title":"The biological role of death and lysis in biofilm development","volume":"5","author":"Bayles","year":"2007","journal-title":"Nat Rev Microbiol"},{"key":"2022030901054618300_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.foodcont.2016.12.016","article-title":"A review of Listeria monocytogenes: an update on outbreaks, virulence, dose-response, ecology, and risk assessments","volume":"75","author":"Buchanan","year":"2017","journal-title":"Food Control"},{"key":"2022030901054618300_bib4","doi-asserted-by":"crossref","first-page":"5522","DOI":"10.1073\/pnas.86.14.5522","article-title":"Intracellular methicillin selection of Listeria monocytogenes mutants unable to replicate in a macrophage cell line","volume":"86","author":"Camilli","year":"1989","journal-title":"Proc Natl Acad Sci"},{"key":"2022030901054618300_bib5","doi-asserted-by":"crossref","first-page":"2758","DOI":"10.1128\/AEM.02844-06","article-title":"A mariner-based transposition system for Listeria monocytogenes","volume":"73","author":"Cao","year":"2007","journal-title":"Appl Environ Microbiol"},{"article-title":"Multistate outbreak of listeriosis linked to commercially produced, prepackaged caramel apples made from Bidart Bros. 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