{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T16:17:00Z","timestamp":1780589820460,"version":"3.54.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100006533","name":"Ministry of Agriculture of the Czech Republic","doi-asserted-by":"crossref","award":["RO0518"],"award-info":[{"award-number":["RO0518"]}],"id":[{"id":"10.13039\/501100006533","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006533","name":"Ministry of Agriculture of the Czech Republic","doi-asserted-by":"crossref","award":["QK1810212"],"award-info":[{"award-number":["QK1810212"]}],"id":[{"id":"10.13039\/501100006533","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Infection with Toxoplasma gondii has usually been connected with consumption of improperly treated meat. However, contaminated water and products of plant origin have emerged as new sources of infection in the last few years. Here, 292 vegetable samples\u2014carrot, cucumber and lettuce\u2014obtained from nine farms in the Czech Republic were examined using triplex real time PCR targeting two specific T. gondii sequences. Irrigation water and water used for washing of vegetables were also included. Overall, a positivity rate of 9.6% was found in vegetables. The concentration varied between 1.31\u00a0\u00d7\u00a0100 and 9.00\u00a0\u00d7\u00a0102 oocysts\/g of sample. A significant difference was found between the positivity of vegetables collected directly from fields and that of vegetables collected from farm storage rooms (4.4\u20138.6% vs 10\u201324.1%, respectively). All samples of irrigation water and water used to rinse vegetables were negative. Genotyping based on restriction fragment length polymorphism (RFLP) analysis using seven markers revealed the exclusive presence of genotype II.<\/jats:p>","DOI":"10.1093\/femsle\/fnz170","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T18:48:55Z","timestamp":1565203735000},"source":"Crossref","is-referenced-by-count":19,"title":["<i>Toxoplasma gondii<\/i> in vegetables from fields and farm storage facilities in the Czech Republic"],"prefix":"10.1093","volume":"366","author":[{"given":"Michal","family":"Slany","sequence":"first","affiliation":[{"name":"Department of Food and Feed Safety, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7203-5569","authenticated-orcid":false,"given":"Radka","family":"Dziedzinska","sequence":"additional","affiliation":[{"name":"Department of Food and Feed Safety, Veterinary Research Institute, Hudcova 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