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In Gram-negative bacteria, it is required for the export of folded proteins from the cytoplasm to the periplasm. There are 30 experimentally verified Tat substrates in Salmonella, including hydrogenase subunits, enzymes required for anaerobic respiration and enzymes involved in peptidoglycan remodeling during cell division. Multiple studies have demonstrated the susceptibility of tat mutants to antimicrobial compounds such as SDS and bile; however, in this work, we use growth curves and viable plate counts to demonstrate that cell wall targeting antibiotics (penicillins, carbapenems, cephalosporins and fosfomycin) have increased killing against a \u0394tat strain. Further, we demonstrate that this increased killing is primarily due to defects in translocation of critical Tat substrates: MepK, AmiA, AmiC and SufI. Finally, we show that a \u0394hyaAB \u0394hybABC \u0394hydBC strain has an altered \u0394\u03a8 that impacts proper secretion of critical Tat substrates in aerobic growth conditions.<\/jats:p>","DOI":"10.1093\/femsmc\/xtab004","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T18:49:20Z","timestamp":1618598960000},"source":"Crossref","is-referenced-by-count":8,"title":["Twin-arginine translocation (Tat) mutants in <i>Salmonella enterica<\/i> serovar Typhimurium have increased susceptibility to cell wall targeting antibiotics"],"prefix":"10.1093","volume":"2","author":[{"given":"Adrienne","family":"M\u00a0Brauer","sequence":"first","affiliation":[{"name":"Department of Biology, Southeast Missouri State University , Cape Girardeau, MO 63701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"R\u00a0Rogers","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Midwestern 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