{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:07:42Z","timestamp":1786752462669,"version":"build-2736575974"},"reference-count":88,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":128,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Netherlands Centre of One Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Enterococcus faecium is an opportunistic pathogen able to colonize the intestines of hospitalized patients. This initial colonization is an important step in the downstream pathogenesis, which includes outgrowth of the intestinal microbiota and potential infection of the host. The impact of intestinal overgrowth on host\u2013enterococcal interactions is not well understood. We therefore applied a RNAseq approach in order to unravel the transcriptional dynamics of E. faecium upon co-culturing with human derived colonic epithelium. Co-cultures of colonic epithelium with a hospital-associated vancomycin resistant (vanA-type) E. faecium (VRE) showed that VRE resided on top of the colonic epithelium when analyzed by microscopy. RNAseq revealed that exposure to the colonic epithelium resulted in upregulation of 238 VRE genes compared to the control condition, including genes implicated in pili expression, conjugation (plasmid_2), genes related to sugar uptake, and biofilm formation (chromosome). In total, 260 were downregulated, including the vanA operon located on plasmid_3. Pathway analysis revealed an overall switch in metabolism to amino acid scavenging and reduction. In summary, our study demonstrates that co-culturing of VRE with human colonic epithelium promotes an elaborate gene response in VRE, enhancing our insight in host\u2013E. faecium interactions, which might facilitate the design of novel anti-infectivity strategies.<\/jats:p>","DOI":"10.1093\/femsmc\/xtae014","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:59:02Z","timestamp":1715129942000},"source":"Crossref","is-referenced-by-count":6,"title":["Colonization of vancomycin-resistant <i>Enterococcus faecium<\/i> in human-derived colonic epithelium: unraveling the transcriptional dynamics of host\u2013enterococcal interactions"],"prefix":"10.1093","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2020-2702","authenticated-orcid":false,"given":"Paul B","family":"Stege","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, UMC Utrecht , Utrecht, 3584CX , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M","family":"Beekman","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht, 3584CX , The Netherlands"},{"name":"Regenerative Medicine Utrecht, University Medical Center Utrecht, Utrecht University , Utrecht, 3584CX , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoni P A","family":"Hendrickx","sequence":"additional","affiliation":[{"name":"Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM) , 3721MA , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"van\u00a0Eijk","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, UMC Utrecht , Utrecht, 3584CX , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malbert R C","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, UMC Utrecht , Utrecht, 3584CX , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia W F","family":"Suen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht, 3584CX , The Netherlands"},{"name":"Regenerative Medicine Utrecht, University Medical Center Utrecht, Utrecht University , Utrecht, 3584CX , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annelotte M","family":"Vonk","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University , Utrecht, 3584CX , The Netherlands"},{"name":"Regenerative 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