{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:22:44Z","timestamp":1792758164896,"version":"4.5.0"},"reference-count":67,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":155,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007924","name":"University of Louisville","doi-asserted-by":"publisher","award":["P20GM125504"],"award-info":[{"award-number":["P20GM125504"]}],"id":[{"id":"10.13039\/100007924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Vancomycin-resistant enterococci (VRE) pose a serious threat to public health because of their limited treatment options. Therefore, there is an increasing need to identify novel targets to develop new drugs. Here, we examined the roles of the universal PTS components, PtsI and PtsH, in Enterococcus faecium to determine their roles in carbon metabolism, biofilm formation, stress response, and the ability to compete in the gastrointestinal tract. Clean deletion of ptsHI resulted in a significant reduction in the ability to import and metabolize simple sugars, attenuated growth rate, reduced biofilm formation, and decreased competitive fitness both in vitro and in vivo. However, no significant difference in stress survival was observed when compared with the wild type. These results suggest that targeting universal or specific PTS may provide a novel treatment strategy by reducing the fitness of E. faecium.<\/jats:p>","DOI":"10.1093\/femsmc\/xtae018","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T01:47:29Z","timestamp":1717465649000},"source":"Crossref","is-referenced-by-count":8,"title":["The role of the universal sugar transport system components PtsI (EI) and PtsH (HPr) in <i>Enterococcus faecium<\/i>"],"prefix":"10.1093","volume":"5","author":[{"given":"Michelle","family":"Hallenbeck","sequence":"first","affiliation":[{"name":"Department of Microbiology & Immunology, University of Louisville , Louisville, KY 40202 , United States"},{"name":"Center for Predictive Medicine, University of Louisville , Louisville, KY 40202 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Chua","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, University of Louisville , Louisville, KY 40202 , United States"},{"name":"Center for Predictive Medicine, University of Louisville , Louisville, KY 40202 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2479-5189","authenticated-orcid":false,"given":"James","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, University of Louisville , Louisville, KY 40202 , United States"},{"name":"Center for Predictive Medicine, University of Louisville , Louisville, KY 40202 , United States"},{"name":"Center for Microbiomics, Inflammation and Pathogenicity, University of Louisville , Louisville, KY 40202 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,3]]},"reference":[{"key":"2024071013215282800_bib1","doi-asserted-by":"crossref","first-page":"8926","DOI":"10.1038\/s41598-019-45441-3","article-title":"Intestinal translocation of enterococci requires a threshold level of enterococcal overgrowth in the lumen","volume":"9","author":"Archambaud","year":"2019","journal-title":"Sci Rep"},{"key":"2024071013215282800_bib2","first-page":"65","article-title":"The cold shock response of Lactobacillus casei: relation between HPr phosphorylation and resistance to freeze\/thaw cycles","volume":"13","author":"Beaufils","year":"2007","journal-title":"J Mol Microbiol Biotechnol"},{"key":"2024071013215282800_bib3","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/mmi.14413","article-title":"Influence of the phosphoenolpyruvate:carbohydrate phosphotransferase system on toxin gene expression and virulence in Bacillus anthracis","volume":"113","author":"Bier","year":"2020","journal-title":"Mol Microbiol"},{"key":"2024071013215282800_bib4","doi-asserted-by":"crossref","first-page":"e0012321","DOI":"10.1128\/MMBR.00123-21","article-title":"Sugar-phosphate toxicities","volume":"85","author":"Boulanger","year":"2021","journal-title":"Microbiol Mol Biol Rev"},{"key":"2024071013215282800_bib5","doi-asserted-by":"crossref","first-page":"711","DOI":"10.3390\/antibiotics11060711","article-title":"Experimental evolution of copper resistance in Escherichia coli produces evolutionary trade-offs in the antibiotics chloramphenicol, bacitracin, and sulfonamide","volume":"11","author":"Boyd","year":"2022","journal-title":"Antibiotics (Basel)"},{"key":"2024071013215282800_bib6","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s41579-018-0107-z","article-title":"Biofilm-associated infection by enterococci","volume":"17","author":"Ch'ng","year":"2019","journal-title":"Nat Rev Micro"},{"key":"2024071013215282800_bib7","doi-asserted-by":"crossref","first-page":"e0242721","DOI":"10.1128\/spectrum.02427-21","article-title":"Rapid, efficient, and cost-effective gene editing of Enterococcus faecium with CRISPR-Cas12a","volume":"10","author":"Chua","year":"2022","journal-title":"Microbiol Spectr"},{"key":"2024071013215282800_bib8","doi-asserted-by":"crossref","first-page":"112122","DOI":"10.1016\/j.jinorgbio.2023.112122","article-title":"The role of CopA in Streptococcus pyogenes copper homeostasis and virulence","volume":"240","author":"Dao","year":"2023","journal-title":"J Inorg Biochem"},{"key":"2024071013215282800_bib9","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1111\/mmi.13349","article-title":"The phosphocarrier protein HPr of Neisseria meningitidis interacts with the transcription regulator CrgA and its deletion affects capsule production, cell adhesion, and virulence","volume":"100","author":"Derkaoui","year":"2016","journal-title":"Mol Microbiol"},{"key":"2024071013215282800_bib10","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1128\/MMBR.00024-06","article-title":"How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria","volume":"70","author":"Deutscher","year":"2006","journal-title":"Microbiol Mol Biol Rev"},{"key":"2024071013215282800_bib11","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1093\/advances\/nmz118","article-title":"Adaptation of the gut microbiota to modern dietary sugars and sweeteners","volume":"11","author":"Di\u00a0Rienzi","year":"2020","journal-title":"Adv Nutr"},{"key":"2024071013215282800_bib12","doi-asserted-by":"crossref","first-page":"2701","DOI":"10.3390\/cells9122701","article-title":"Diet rich in simple sugars promotes pro-inflammatory response via gut microbiota alteration and TLR4 signaling","volume":"9","author":"Fajstova","year":"2020","journal-title":"Cells"},{"key":"2024071013215282800_bib13","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.GPP3-0053-2018","article-title":"Pathogenicity of enterococci","volume":"7","author":"Fiore","year":"2019","journal-title":"Microbiol Spectr"},{"key":"2024071013215282800_bib14","doi-asserted-by":"crossref","first-page":"e1010617","DOI":"10.1371\/journal.ppat.1010617","article-title":"Copper microenvironments in the human body define patterns of copper adaptation in pathogenic bacteria","volume":"18","author":"Focarelli","year":"2022","journal-title":"PLoS Pathog"},{"key":"2024071013215282800_bib15","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.jmb.2017.02.006","article-title":"Sophisticated regulation of transcriptional factors by the bacterial phosphoenolpyruvate: sugar phosphotransferase system","volume":"429","author":"Galinier","year":"2017","journal-title":"J Mol Biol"},{"key":"2024071013215282800_bib16","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.mib.2017.11.030","article-title":"Evolution of virulence in Enterococcus faecium, a hospital-adapted opportunistic pathogen","volume":"41","author":"Gao","year":"2018","journal-title":"Curr Opin Microbiol"},{"key":"2024071013215282800_bib17","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1128\/IAI.01271-13","article-title":"The phosphoenolpyruvate phosphotransferase system in group A Streptococcus acts to reduce streptolysin S activity and lesion severity during soft tissue infection","volume":"82","author":"Gera","year":"2014","journal-title":"Infect Immun"},{"key":"2024071013215282800_bib18","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s00284-004-4204-z","article-title":"Catabolite repression and virulence gene expression in Listeria monocytogenes","volume":"49","author":"Gilbreth","year":"2004","journal-title":"Curr Microbiol"},{"key":"2024071013215282800_bib19","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.mib.2013.01.006","article-title":"Genomic transition of enterococci from gut commensals to leading causes of multidrug-resistant hospital infection in the antibiotic era","volume":"16","author":"Gilmore","year":"2013","journal-title":"Curr Opin Microbiol"},{"key":"2024071013215282800_bib20","doi-asserted-by":"crossref","first-page":"e01147","DOI":"10.1128\/AEM.01147-20","article-title":"Enterococcus faecalis maltodextrin gene regulation by combined action of maltose gene regulator MalR and pleiotropic regulator CcpA","volume":"86","author":"Grand","year":"2020","journal-title":"Appl Environ Microb"},{"key":"2024071013215282800_bib21","doi-asserted-by":"crossref","first-page":"4611","DOI":"10.1128\/jb.178.15.4611-4619.1996","article-title":"Cold shock stress-induced proteins in Bacillus subtilis","volume":"178","author":"Graumann","year":"1996","journal-title":"J Bacteriol"},{"key":"2024071013215282800_bib22","doi-asserted-by":"crossref","first-page":"4166","DOI":"10.1128\/IAI.00711-10","article-title":"Enterococcal biofilm formation and virulence in an optimized murine model of foreign body-associated urinary tract infections","volume":"78","author":"Guiton","year":"2010","journal-title":"Infect Immun"},{"key":"2024071013215282800_bib23","doi-asserted-by":"crossref","first-page":"8233","DOI":"10.1128\/JB.01205-07","article-title":"Enterococcal surface protein esp is important for biofilm formation of Enterococcus faecium E1162","volume":"189","author":"Heikens","year":"2007","journal-title":"J Bacteriol"},{"key":"2024071013215282800_bib24","doi-asserted-by":"crossref","first-page":"5097","DOI":"10.1128\/IAI.00275-09","article-title":"SgrA, a nidogen-binding LPXTG surface adhesin implicated in biofilm formation, and EcbA, a collagen binding MSCRAMM, are two novel adhesins of hospital-acquired Enterococcus faecium","volume":"77","author":"Hendrickx","year":"2009","journal-title":"Infect Immun"},{"key":"2024071013215282800_bib25","first-page":"65","article-title":"A simple sequentially rejective multiple test procedure","volume":"6","author":"Holm","year":"1979","journal-title":"Scand J Stat"},{"key":"2024071013215282800_bib26","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jmii.2017.01.007","article-title":"Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation","volume":"51","author":"Horng","year":"2018","journal-title":"J Microbiol Immunol Infect"},{"key":"2024071013215282800_bib27","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1128\/IAI.01356-09","article-title":"Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germfree mouse intestine","volume":"78","author":"Houot","year":"2010","journal-title":"Infect Immun"},{"key":"2024071013215282800_bib28","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.1128\/JB.00213-10","article-title":"The phosphoenolpyruvate phosphotransferase system regulates vibrio cholerae biofilm formation through multiple independent pathways","volume":"192","author":"Houot","year":"2010","journal-title":"J Bacteriol"},{"key":"2024071013215282800_bib29","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1128\/JB.01410-07","article-title":"A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm","volume":"190","author":"Houot","year":"2008","journal-title":"J Bacteriol"},{"key":"2024071013215282800_bib30","doi-asserted-by":"crossref","first-page":"eaay6218","DOI":"10.1126\/scitranslmed.aay6218","article-title":"Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice","volume":"12","author":"Khan","year":"2020","journal-title":"Sci Transl Med"},{"key":"2024071013215282800_bib31","doi-asserted-by":"crossref","first-page":"113769","DOI":"10.1016\/j.lwt.2022.113769","article-title":"The accurate identification and quantification of six Enterococcus species using quantitative polymerase chain reaction based novel DNA markers","volume":"166","author":"Kim","year":"2022","journal-title":"LWT"},{"key":"2024071013215282800_bib32","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1099\/mic.0.26406-0","article-title":"Effect of enzyme I of the bacterial phosphoenolpyruvate : sugar phosphotransferase system (PTS) on virulence in a murine model","volume":"149","author":"Kok","year":"2003","journal-title":"Microbiology (Reading)"},{"key":"2024071013215282800_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijmm.2010.04.021","article-title":"Sugar metabolism, an additional virulence factor in enterobacteria","volume":"301","author":"Le\u00a0Bouguenec","year":"2011","journal-title":"Int J Med Microbiol"},{"key":"2024071013215282800_bib34","doi-asserted-by":"crossref","first-page":"e00534","DOI":"10.1128\/mBio.00534-13","article-title":"Emergence of epidemic multidrug-resistant Enterococcus faecium from animal and commensal strains","volume":"4","author":"Lebreton","year":"2013","journal-title":"mBio"},{"key":"2024071013215282800_bib35","doi-asserted-by":"crossref","first-page":"e1002834","DOI":"10.1371\/journal.ppat.1002834","article-title":"AsrR is an oxidative stress sensing regulator modulating Enterococcus faecium opportunistic traits, antimicrobial resistance, and pathogenicity","volume":"8","author":"Lebreton","year":"2012","journal-title":"PLoS Pathog"},{"key":"2024071013215282800_bib36","doi-asserted-by":"crossref","first-page":"101587","DOI":"10.1016\/j.jbc.2022.101587","article-title":"Regulation of DNA-binding activity of the Staphylococcus aureus catabolite control protein A by copper (II)-mediated oxidation","volume":"298","author":"Liao","year":"2022","journal-title":"J Biol Chem"},{"key":"2024071013215282800_bib37","doi-asserted-by":"crossref","first-page":"M1392","DOI":"10.1111\/1750-3841.12181","article-title":"Construction of Listeria monocytogenes mutants with in-frame deletions in the phosphotransferase transport system (PTS) and analysis of their growth under stress conditions","volume":"78","author":"Liu","year":"2013","journal-title":"J Food Sci"},{"key":"2024071013215282800_bib38","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.3389\/fmicb.2017.01611","article-title":"LMOf2365_0442 Encoding for a fructose specific PTS permease IIA may Be required for virulence in L. monocytogenes strain F2365","volume":"8","author":"Liu","year":"2017","journal-title":"Front Microbiol"},{"key":"2024071013215282800_bib39","doi-asserted-by":"crossref","first-page":"7826","DOI":"10.1128\/JB.187.22.7826-7839.2005","article-title":"Catabolite repression and activation in Bacillus subtilis: dependency on CcpA, HPr, and HprK","volume":"187","author":"Lorca","year":"2005","journal-title":"J Bacteriol"},{"key":"2024071013215282800_bib40","doi-asserted-by":"crossref","first-page":"e0059721","DOI":"10.1128\/iai.00597-21","article-title":"N,N-dimethyldithiocarbamate elicits pneumococcal hypersensitivity to copper and macrophage-mediated clearance","volume":"90","author":"Menghani","year":"2022","journal-title":"Infect Immun"},{"key":"2024071013215282800_bib41","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1128\/JB.00972-06","article-title":"Interference of components of the phosphoenolpyruvate phosphotransferase system with the central virulence gene regulator PrfA of Listeria monocytogenes","volume":"189","author":"Mertins","year":"2007","journal-title":"J Bacteriol"},{"key":"2024071013215282800_bib42","doi-asserted-by":"crossref","first-page":"e0050821","DOI":"10.1128\/mSystems.00508-21","article-title":"AMiGA: software for automated analysis of microbial growth assays","volume":"6","author":"Midani","year":"2021","journal-title":"Msystems"},{"key":"2024071013215282800_bib43","doi-asserted-by":"crossref","first-page":"5425","DOI":"10.1111\/1462-2920.16244","article-title":"Phosphotransferase system sugars immediately induce mutations of Cra in an Escherichia coli ptsH mutant","volume":"24","author":"Min","year":"2022","journal-title":"Environ Microbiol"},{"key":"2024071013215282800_bib44","first-page":"20","article-title":"The phosphotransferase system of Lactobacillus casei: regulation of carbon metabolism and connection to cold shock response","volume":"12","author":"Monedero","year":"2007","journal-title":"J Mol Microbiol Biotechnol"},{"key":"2024071013215282800_bib45","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1093\/infdis\/jiv597","article-title":"Gastrointestinal tract colonization dynamics by different Enterococcus faecium clades","volume":"213","author":"Montealegre","year":"2016","journal-title":"J Infect Dis"},{"key":"2024071013215282800_bib46","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1093\/infdis\/jiw108","article-title":"Genome-wide screening identifies phosphotransferase system permease BepA to Be involved in Enterococcus faecium endocarditis and biofilm formation","volume":"214","author":"Paganelli","year":"2016","journal-title":"J Infect Dis"},{"key":"2024071013215282800_bib47","doi-asserted-by":"crossref","first-page":"e00154","DOI":"10.1128\/mBio.00154-13","article-title":"Enterococcus faecium biofilm formation: identification of major autolysin AtlAEfm, associated Acm surface localization, and AtlAEfm-independent extracellular DNA release","volume":"4","author":"Paganelli","year":"2013","journal-title":"mBio"},{"key":"2024071013215282800_bib48","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.resmic.2017.03.003","article-title":"Phosphotransferase systems in Enterococcus faecalis OG1RF enhance anti-stress capacity in vitro and in vivo","volume":"168","author":"Peng","year":"2017","journal-title":"Res Microbiol"},{"key":"2024071013215282800_bib49","first-page":"125","article-title":"Recent developments in bacterial cold-shock response","volume":"6","author":"Phadtare","year":"2004","journal-title":"Curr Iss Mol Biol"},{"key":"2024071013215282800_bib50","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/1469-0691.12236","article-title":"Incidence, clinical characteristics and 30-day mortality of enterococcal bacteraemia in Denmark 2006-2009: a population-based cohort study","volume":"20","author":"Pinholt","year":"2014","journal-title":"Clin Microbiol Infect"},{"key":"2024071013215282800_bib51","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1038\/oby.2003.179","article-title":"The sweetening of the world's diet","volume":"11","author":"Popkin","year":"2003","journal-title":"Obes Res"},{"key":"2024071013215282800_bib52","doi-asserted-by":"crossref","first-page":"612","DOI":"10.3389\/fmicb.2019.00612","article-title":"The combined effect of cold and copper stresses on the proliferation and transcriptional response of Listeria monocytogenes","volume":"10","author":"Quesille-Villalobos","year":"2019","journal-title":"Front Microbiol"},{"key":"2024071013215282800_bib53","doi-asserted-by":"crossref","first-page":"e0146021","DOI":"10.1371\/journal.pone.0146021","article-title":"Dose-response analysis using R","volume":"10","author":"Ritz","year":"2015","journal-title":"PLoS One"},{"key":"2024071013215282800_bib54","doi-asserted-by":"crossref","first-page":"236","DOI":"10.4161\/viru.1.4.11966","article-title":"Characterization of the ebp(fm) pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection","volume":"1","author":"Sillanpaa","year":"2010","journal-title":"Virulence"},{"key":"2024071013215282800_bib55","doi-asserted-by":"crossref","first-page":"3580","DOI":"10.1128\/IAI.01911-14","article-title":"CcpA is important for growth and virulence of Enterococcus faecium","volume":"82","author":"Somarajan","year":"2014","journal-title":"Infect Immun"},{"key":"2024071013215282800_bib56","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1093\/jac\/dkt270","article-title":"Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis","volume":"68","author":"Starikova","year":"2013","journal-title":"J Antimicrob Chemother"},{"key":"2024071013215282800_bib57","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1126\/science.aax3760","article-title":"Lactose drives Enterococcus expansion to promote graft-versus-host disease","volume":"366","author":"Stein-Thoeringer","year":"2019","journal-title":"Science"},{"key":"2024071013215282800_bib58","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1093\/cid\/cis580","article-title":"Intestinal domination and the risk of bacteremia in patients undergoing allogeneic hematopoietic stem cell transplantation","volume":"55","author":"Taur","year":"2012","journal-title":"Clin Infect Dis"},{"key":"2024071013215282800_bib59","doi-asserted-by":"crossref","first-page":"637656","DOI":"10.3389\/fmicb.2021.637656","article-title":"Genomic insights into last-line antimicrobial resistance in multidrug-resistant Staphylococcus and vancomycin-resistant Enterococcus","volume":"12","author":"Turner","year":"2021","journal-title":"Front Microbiol"},{"key":"2024071013215282800_bib60","doi-asserted-by":"crossref","first-page":"6672636","DOI":"10.1155\/2020\/6672636","article-title":"High-fructose diet increases inflammatory cytokines and alters gut microbiota composition in rats","volume":"2020","author":"Wang","year":"2020","journal-title":"Mediat Inflamm"},{"key":"2024071013215282800_bib61","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.bbmt.2007.01.078","article-title":"Colonization, bloodstream infection, and mortality caused by vancomycin-resistant Enterococcus early after allogeneic hematopoietic stem cell transplant","volume":"13","author":"Weinstock","year":"2007","journal-title":"Biol Blood Marrow Transplant"},{"key":"2024071013215282800_bib62","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.21105\/joss.01686","article-title":"Welcome to the Tidyverse","volume":"4","author":"Wickham","year":"2019","journal-title":"J Open Source Software"},{"key":"2024071013215282800_bib63","doi-asserted-by":"crossref","first-page":"110165","DOI":"10.1016\/j.ijfoodmicro.2023.110165","article-title":"Trehalose transport occurs via TreB in Listeria monocytogenes and it influences biofilm development and acid resistance","volume":"394","author":"Wu","year":"2023","journal-title":"Int J Food Microbiol"},{"key":"2024071013215282800_bib64","doi-asserted-by":"crossref","first-page":"22602","DOI":"10.1007\/s11356-021-17510-6","article-title":"Excess copper promotes catabolic activity of gram-positive bacteria and resistance of gram-negative bacteria but inhibits fungal community in soil","volume":"29","author":"Yang","year":"2022","journal-title":"Environ Sci Pollut Res Int"},{"key":"2024071013215282800_bib65","doi-asserted-by":"crossref","first-page":"e2118566119","DOI":"10.1073\/pnas.2118566119","article-title":"A broadly applicable, stress-mediated bacterial death pathway regulated by the phosphotransferase system (PTS) and the cAMP-crp cascade","volume":"119","author":"Zeng","year":"2022","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024071013215282800_bib66","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1093\/infdis\/jit076","article-title":"Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment","volume":"207","author":"Zhang","year":"2013","journal-title":"J Infect Dis"},{"key":"2024071013215282800_bib67","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/j.1462-2920.2010.02355.x","article-title":"A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source","volume":"13","author":"Zhang","year":"2011","journal-title":"Environ Microbiol"}],"container-title":["FEMS Microbes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/femsmicrobes\/advance-article-pdf\/doi\/10.1093\/femsmc\/xtae018\/58065186\/xtae018.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsmicrobes\/article-pdf\/doi\/10.1093\/femsmc\/xtae018\/58496939\/xtae018.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsmicrobes\/article-pdf\/doi\/10.1093\/femsmc\/xtae018\/58496939\/xtae018.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T15:28:31Z","timestamp":1720625311000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsmicrobes\/article\/doi\/10.1093\/femsmc\/xtae018\/7687149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":67,"URL":"https:\/\/doi.org\/10.1093\/femsmc\/xtae018","relation":{},"ISSN":["2633-6685"],"issn-type":[{"value":"2633-6685","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024]]},"published":{"date-parts":[[2024]]},"article-number":"xtae018"}}