{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:55:49Z","timestamp":1789516549148,"version":"build-2803163510"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,3,28]],"date-time":"2020-03-28T00:00:00Z","timestamp":1585353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100008425","name":"Conseller\u00eda de Cultura, Educaci\u00f3n e Ordenaci\u00f3n Universitaria, Xunta de Galicia","doi-asserted-by":"publisher","award":["ED481B-2018\/018"],"award-info":[{"award-number":["ED481B-2018\/018"]}],"id":[{"id":"10.13039\/501100008425","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The prophage BTP1 is highly conserved among strains of the pathogenic lineage Salmonella Typhimurium ST313. We aimed to analyze the role of BTP1 and the gene bstA(BTP1-encoded) in virulence of S. Typhimurium D23580, the ST313 lineage 2 reference strain. The deletion mutant D23580\u0394bstA showed significantly higher replication and survival rates within human-derived THP-1 macrophages than the wild-type (WT) strain, while the mutant isolate \u0394BTP1, lacking the full prophage, did not significantly differ from the WT. Interestingly, during mice infection, \u0394BTP1 yielded significantly higher counts in all tested organs [spleens, livers and mesenteric lymph nodes (MLN)] than the WT, and organs were significantly enlarged compared to WT-infected animals. D23580\u0394bstA significantly outcompeted the WT during competitive infection of mice, and yielded significantly enlarged spleens and MLN compared to WT-infected animals during single strain infection. Moreover, increased cellular infiltration and focal necrosis were observed in the liver samples of mice infected with D23580\u0394bstA and \u0394BTP1 compared to WT-infected animals. In conclusion, removal of the gene bstA and the prophage BTP1 in S. Typhimurium D23580 led to increased virulence in mice, demonstrating that bstA is an antivirulence gene.<\/jats:p>","DOI":"10.1093\/femspd\/ftaa019","type":"journal-article","created":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T12:07:30Z","timestamp":1584965250000},"source":"Crossref","is-referenced-by-count":12,"title":["Association of the prophage BTP1 and the prophage-encoded gene,<i>bstA<\/i>, with antivirulence of<i>Salmonella<\/i>Typhimurium ST313"],"prefix":"10.1093","volume":"78","author":[{"given":"Malene R","family":"Spiegelhauer","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5459-8325","authenticated-orcid":false,"given":"Vanesa","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal 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