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To characterize the effect of glycogen synthase A deficiency on the pathogenicity of C. muridarum in the genital tract, BALB\/c mice were intravaginally inoculated with wild-type, plasmid-free and glycogen synthase A-deficient C. muridarum, and the genital tract tissue was isolated to assess the severity of hydrosalpinx and the levels of oviduct dilatation at day 60 after infection. The glycogen storage capacity and in vitro infection ability of different C. muridarum strains were analyzed by periodic acid-Schiff staining and quantification of progeny elementary body(EB) formation. The tissue homogenate was used to determine the recovery of different C. muridarum strains. The results show that glycogen synthase A-deficient C. muridarum induced reduction of hydrosalpinx and attenuated the extent of oviduct dilatation in mice, and exhibited reduced growth and proliferation in the mouse lower genital tract. In addition, glycogen synthase A point mutations at different sites reduced the glycogen storage capacity and in vitro infectivity of C. muridarum to different degrees. Glycogen synthase A deficiency also reduced the host inflammatory reaction and ascending infection of C. muridarum.<\/jats:p>","DOI":"10.1093\/femspd\/ftaa027","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T11:29:08Z","timestamp":1591961348000},"source":"Crossref","is-referenced-by-count":6,"title":["GlgA plays an important role in the induction of hydrosalpinx by<i>Chlamydia muridarum<\/i>"],"prefix":"10.1093","volume":"78","author":[{"given":"Chunmin","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Dermatovenereology, Tianjin Medical University General Hospital, 154 Anshan Rd., Tianjin 300052, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoqing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology, Tianjin Medical University General Hospital, 154 Anshan Rd., Tianjin 300052, PR 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