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Aging is associated with immune system dysfunctions that impair responses to vaccines and infections. Therefore, immunization of middle-aged individuals could be beneficial. This study aims to evaluate the immunogenicity of N. meningitidis B outer membrane vesicles (OMVs) complexed to two different adjuvants. Middle-aged BALB\/c and A\/Sn mice were immunized and subsequent immune response was assessed by ELISA, immunoblotting and ELISpot. IgG levels were similar between the animals immunized with OMVs complexed to adjuvants. A total of 235 days after the last immunization only A\/Sn mice presented higher IgG levels than those observed in the baseline, especially the group immunized with OMVs and aluminum hydroxide. The predominant IgG subclasses were IgG2a and IgG2b. Immunization with the three-dose regimen generated IgG antibodies that recognized a variety of antigens present in the homologous and heterologous meningococcal OMVs evaluated. There was an increase in the frequency of antigen-specific IFN-\u03b3 secreting splenocytes, after in vitro stimulation, in mice immunized with OMVs and adjuvants compared to the control group, almost 1 year after the last immunization. Both adjuvants showed similar performance. Immunization of middle-aged mice has generated a robust immune response and it appears to be advantageous.<\/jats:p>","DOI":"10.1093\/femspd\/ftaa028","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T15:09:39Z","timestamp":1594134579000},"source":"Crossref","is-referenced-by-count":10,"title":["Immune responses of meningococcal B outer membrane vesicles in middle-aged mice"],"prefix":"10.1093","volume":"78","author":[{"given":"Gabriela","family":"Trzewikoswki\u00a0de\u00a0Lima","sequence":"first","affiliation":[{"name":"Department of Immunology, Adolfo Lutz Institute , Av. Dr. Arnaldo 355, 11 floor, Sao Paulo, SP,","place":["Brazil"]},{"name":"Interunits Post-Graduate Program in Biotechnology, University of Sao Paulo , Sao Paulo, SP,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais Sousa","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Immunology, Adolfo Lutz Institute , Av. Dr. Arnaldo 355, 11 floor, Sao Paulo, SP,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Izeli","family":"Portilho","sequence":"additional","affiliation":[{"name":"Department of Immunology, Adolfo Lutz Institute , Av. Dr. Arnaldo 355, 11 floor, Sao Paulo, SP,","place":["Brazil"]},{"name":"Interunits Post-Graduate Program in Biotechnology, University of Sao Paulo , Sao Paulo, SP,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor Araujo","family":"Correa","sequence":"additional","affiliation":[{"name":"Department of Immunology, Adolfo Lutz Institute , Av. Dr. Arnaldo 355, 11 floor, Sao Paulo, SP,","place":["Brazil"]},{"name":"Interunits Post-Graduate Program in Biotechnology, University of Sao Paulo , Sao Paulo, SP,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuelle Baldo","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Embrapa Southern Region Animal Husbandry , Bag\u00e9, RS,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"De\u00a0Gaspari","sequence":"additional","affiliation":[{"name":"Department of Immunology, Adolfo Lutz Institute , Av. 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