{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T18:15:42Z","timestamp":1774548942568,"version":"3.50.1"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Institute of Microbiology of the University of Lausanne"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Objectives:In order to cope with the rapid spread of the COVID-19 pandemic, we introduced on our in-house high-throughput molecular diagnostic platform (MDx Platform) a real-time reverse transcriptase PCR (RT-PCR) to detect the SARS-CoV-2 from any clinical specimens. The aim of this study was to compare the RT-PCR results obtain with the MDx Platform and the commercial assay cobas SARS-CoV-2 (Roche) on nasopharyngeal swab and other clinical specimens including sputum, bronchial aspirate, bronchoalveolar lavage and anal swabs. Methods: Samples received in our laboratory from patients suspected of COVID-19 (n = 262) were tested in parallel with our MDx platform SARS-CoV-2 PCR and with the cobas SARS-CoV-2 test. Results: The overall agreement between the two tests for all samples tested was 99.24% (260\/262), which corresponded to agreements of 100% (178\/178) on nasopharyngeal swabs, 95.45% (42\/44) on lower respiratory tract specimen with discordant resultS obtained for very high cycle threshold (Ct) value and 100% (40\/40) on anorectal swabs. The Ct values for nasopharyngeal swabs displayed an excellent correlation (R2 &amp;gt; 96%) between both tests. Conclusions: The high agreements between the cobas SARS-CoV-2 test and the MDx platform supports the use of both methods for the diagnostic of COVID-19 on various clinical samples. Very few discrepant results may occur at very low viral load.<\/jats:p>","DOI":"10.1093\/femspd\/ftaa061","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T19:14:54Z","timestamp":1602011694000},"source":"Crossref","is-referenced-by-count":48,"title":["Comparison of SARS-CoV-2 RT-PCR on a high-throughput molecular diagnostic platform and the cobas SARS-CoV-2 test for the diagnostic of COVID-19 on various clinical samples"],"prefix":"10.1093","volume":"78","author":[{"given":"Onya","family":"Opota","sequence":"first","affiliation":[{"name":"Institute of Microbiology, University Hospital Center and University of Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9","family":"Brouillet","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, University Hospital Center and University of Lausanne, 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