{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:32:48Z","timestamp":1788568368176,"version":"build-2803163510"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Background: despite being in the 5th month of pandemic, knowledge with respect to viral dynamics, infectivity and RT-PCR positivity continues to evolve.<\/jats:p>\n                  <jats:p>Aim: to analyse the SARS CoV-2 nucleic acid RT-PCR profiles in COVID-19 patients.<\/jats:p>\n                  <jats:p>Design: it was a retrospective, observational study conducted at COVID facilities under AIIMS, New Delhi.<\/jats:p>\n                  <jats:p>Methods: patients admitted with laboratory confirmed COVID-19 were eligible for enrolment. Patients with incomplete details, or only single PCR tests were excluded. Data regarding demographic details, comorbidities, treatment received and results of SARS-CoV-2 RT-PCR performed on nasopharyngeal and oropharyngeal swabs, collected at different time points, was retrieved from the hospital records.<\/jats:p>\n                  <jats:p>Results: a total of 298 patients were included, majority were males (75\u00b78%) with mean age of 39\u00b707 years (0\u00b76\u201388 years). The mean duration from symptom onset to first positive RT-PCR was 4\u00b77 days (SD 3\u00b767), while that of symptom onset to last positive test was 17\u00b783 days (SD 6\u00b722). Proportions of positive RT-PCR tests were 100%, 49%, 24%, 8\u00b77% and 20\u00b76% in the 1st, 2nd, 3rd, 4th and &amp;gt;4\u00a0weeks of illness. A total of 12 symptomatic patients had prolonged positive test results even after 3 weeks of symptom onset. Age &amp;gt; = 60 years was associated with prolonged RT-PCR positivity (statistically significant).<\/jats:p>\n                  <jats:p>Conclusion: this study showed that the average period of PCR positivity is more than 2 weeks in COVID-19 patients; elderly patients have prolonged duration of RT-PCR positivity and requires further follow up.<\/jats:p>","DOI":"10.1093\/femspd\/ftaa064","type":"journal-article","created":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T15:35:24Z","timestamp":1602516924000},"source":"Crossref","is-referenced-by-count":6,"title":["SARS-CoV-2 RT-PCR profile in 298 Indian COVID-19 patients: a retrospective observational study"],"prefix":"10.1093","volume":"79","author":[{"given":"Bisakh","family":"Bhattacharya","sequence":"first","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, Medicine Office, 3rd floor, Teaching block, AIIMS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, Medicine Office, 3rd floor, Teaching block, AIIMS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ved Prakash","family":"Meena","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, Medicine Office, 3rd floor, Teaching block, AIIMS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manish","family":"Soneja","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, Medicine Office, 3rd floor, Teaching block, AIIMS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Teaching block, 2nd floor, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rojaleen","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Teaching block, 2nd floor, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashit","family":"Xess","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Teaching block, 2nd floor, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazneen","family":"Arif","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Teaching block, 2nd floor, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh","family":"Vig","sequence":"additional","affiliation":[{"name":"Department of Onco-anesthesia and Palliative Medicine, Dr. B.R.A.I.R.C.H, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vandana","family":"Rastogi","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavan","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushma","family":"Bhatnagar","sequence":"additional","affiliation":[{"name":"Department of Onco-anesthesia and Palliative Medicine, Dr. B.R.A.I.R.C.H, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anant","family":"Mohan","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveet","family":"Wig","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, Medicine Office, 3rd floor, Teaching block, AIIMS, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalit","family":"Dar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Teaching block, 2nd floor, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"key":"2021010923441556200_bib1","author":"#IndiaFightsCorona COVID-19 [Internet]","year":"2020"},{"key":"2021010923441556200_bib2","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1001\/jama.2020.2565","article-title":"Presumed asymptomatic carrier transmission of COVID-19","volume":"323","author":"Bai","year":"2020","journal-title":"JAMA"},{"key":"2021010923441556200_bib3","author":"Discharge criteria for confirmed COVID-19 cases \u2013 When is it safe to discharge COVID-19 cases from the hospital or end home isolation? Discharge criteria for confirmed COVID-19 cases.pdf"},{"key":"2021010923441556200_bib4","author":"Laboratory testing for 2019 novel coronavirus (2019-nCoV) in suspected human cases [Internet]","year":"2020"},{"key":"2021010923441556200_bib11","first-page":"Apr 27 [cited 2020 Oct 23];1","article-title":"Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards","author":"La\u00a0Scola","year":"2020","journal-title":"Eur J Clin Microbiol Infect Dis [Internet]"},{"key":"2021010923441556200_bib5","doi-asserted-by":"crossref","first-page":"108956","DOI":"10.1016\/j.ejrad.2020.108956","article-title":"Asymptomatic SARS-CoV-2 infected patients with persistent negative CT findings","volume":"126","author":"Ling","year":"2020","journal-title":"Eur J Radiol"},{"key":"2021010923441556200_bib6","article-title":"The reproductive number of COVID-19 is higher compared to SARS coronavirus","volume":"27","author":"Liu","year":"2020","journal-title":"J Travel Med [Internet]"},{"key":"2021010923441556200_bib7","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/S1473-3099(20)30232-2","article-title":"Viral dynamics in mild and severe cases of COVID-19","volume":"20","author":"Liu","year":"2020","journal-title":"Lancet Infect Dis"},{"issue":"4","key":"2021010923441556200_bib8","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/S1473-3099(20)30114-6","article-title":"Asymptomatic cases in a family cluster with SARS-CoV-2 infection","volume":"20","author":"Pan","year":"2020","journal-title":"Lancet Infect Dis"},{"key":"2021010923441556200_bib9","author":"ReviseddischargePolicyforCOVID19.pdf [Internet]","year":"2020"},{"key":"2021010923441556200_bib10","author":"RevisedNationalClinicalManagementGuidelineforCOVID19310 32020.pdf","year":"2020"},{"key":"2021010923441556200_bib12","doi-asserted-by":"crossref","first-page":"104346","DOI":"10.1016\/j.jcv.2020.104346","article-title":"Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: a descriptive study","volume":"127","author":"Xiao","year":"2020","journal-title":"J Clin Virol"},{"key":"2021010923441556200_bib13","doi-asserted-by":"crossref","DOI":"10.1093\/cid\/ciaa460","article-title":"Profile of RT-PCR for SARS-CoV-2: a preliminary study from 56 COVID-19 patients","author":"Xiao","year":"2020","journal-title":"Clin Infect Dis"},{"key":"2021010923441556200_bib14","doi-asserted-by":"crossref","DOI":"10.1093\/cid\/ciaa351","article-title":"Factors associated with prolonged viral RNA shedding in patients with coronavirus disease 2019 (COVID-19)","author":"Xu","year":"2020","journal-title":"Clin Infect Dis"},{"key":"2021010923441556200_bib15","doi-asserted-by":"crossref","DOI":"10.1136\/bmj.m1443","article-title":"Viral load dynamics and disease severity in patients infected with SARS-CoV-2 in Zhejiang province, China, January-March 2020: retrospective cohort study","author":"Zheng","year":"2020","journal-title":"BMJ"}],"container-title":["Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/femspd\/advance-article-pdf\/doi\/10.1093\/femspd\/ftaa064\/33885496\/ftaa064.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femspd\/article-pdf\/79\/1\/ftaa064\/35593709\/ftaa064.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/femspd\/article-pdf\/79\/1\/ftaa064\/35593709\/ftaa064.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T21:46:36Z","timestamp":1610574396000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femspd\/article\/doi\/10.1093\/femspd\/ftaa064\/5923555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,14]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1,9]]}},"URL":"https:\/\/doi.org\/10.1093\/femspd\/ftaa064","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.06.19.20135905","asserted-by":"object"}]},"ISSN":["2049-632X"],"issn-type":[{"value":"2049-632X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,2]]},"published":{"date-parts":[[2020,10,14]]},"article-number":"ftaa064"}}