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However, the effective treatment is limited currently. The aim of this study was to investigate the activity of the vaccine including the EV71 polypeptides mixed with a novel adjuvant containing CpG oligodeoxynucleotides (CpG ODNs). After collecting mouse sera, we determined the antibody concentration in serum by enzyme-linked immunosorbent assays (ELISA). Then, CD19+CD27+ B cells in the spleen were analysed by flow cytometry. The assay revealed that a substantial increase in antibody titers was achieved. This indicates a high level of immunogenicity for peptide vaccine and the good stability of adjuvant, also suggests that the combination of vaccine and adjuvant can stimulate the production of high-level antibodies and CD19+CD27+ B lymphocytes in mice. Furthermore, the antibody could effectively identify EV71 inactivated virus. The results demonstrated that the autonomous construction of EV71 polypeptide vaccine had a good immunogenicity. Moreover, the peptide vaccine injection with a novel adjuvant, which is easy to prepare, could cause a high antibody level of EV71 and shown a good application prospect.<\/jats:p>","DOI":"10.1093\/femspd\/ftab057","type":"journal-article","created":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T12:08:16Z","timestamp":1639656496000},"source":"Crossref","is-referenced-by-count":3,"title":["A novel polypeptide vaccine and adjuvant formulation of EV71"],"prefix":"10.1093","volume":"79","author":[{"given":"Zhiang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Xinxiang Medical University , Xinxiang, Henan 453000, P.R.China"},{"name":"Xinxiang Key Laboratory of Tumor Vaccine and Immunotherapy, Xinxiang Medical University , Xinxiang, Henan 453000, P.R.China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Immunology, 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