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In this study, we combine the well-established Toxoplasmagondii infection model with three in vitro macrophage culture systems to delineate the contribution of individual GBP family members to control this apicomplexan parasite. Use of high-throughput imaging assays and genome engineering allowed us to define a role for GBP1, 2 and 5 in parasite infection control. While GBP1 performs a pathogen-proximal, parasiticidal and growth-restricting function through accumulation at the parasitophorous vacuole of intracellular Toxoplasma, GBP2 and GBP5 perform a pathogen-distal, growth-restricting role. We further find that mutants of the GTPase or isoprenylation site of GBP1\/2\/5 affect their normal function in Toxoplasma control by leading to mis-localization of the proteins.<\/jats:p>","DOI":"10.1093\/femspd\/ftab058","type":"journal-article","created":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T15:11:27Z","timestamp":1639753887000},"source":"Crossref","is-referenced-by-count":26,"title":["<i>Toxoplasma<\/i>\n                    -proximal and distal control by GBPs in human macrophages"],"prefix":"10.1093","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8155-0367","authenticated-orcid":false,"given":"Daniel","family":"Fisch","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Edgbaston B15 2TT, UK"},{"name":"Host-Toxoplasma Interaction Laboratory, The Francis Crick Institute, London NW1 1AT, 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