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Although inexpensive, straightforward, and requiring a\u00a0very short time commitment, these models recapitulate neither the functionality of\u00a0multilayered cell types nor the associated microbiome that occurs\u00a0in a human. Animal models have commonly been used to recreate\u00a0the complexity of human infections.\u00a0However, extensive modifications of animal models are required to recreate interactions that resemble those in the human reproductive tract. 3D cell culture models have emerged as alternative means of reproducing vital elements of human infections at a fraction of the cost of animal models and on a scale that allows\u00a0for replicative\u00a0experiments.\u00a0Here, we describe a new 3D model that utilizes transwells with epithelial cells seeded apically and a basolateral extracellular matrix (ECM)-like layer. The model produced\u00a0tissues with morphologic and physiological resemblance to human cervical and vaginal epithelia, including mucus levels produced by cervical cells. Infection by Chlamydia trachomatis and\u00a0Neisseria gonorrhoeae was demonstrated, as well as the growth of bacterial species observed in\u00a0the human vaginal microbiota. This enabled controlled mechanistic analyses of the interactions between host cells, the\u00a0vaginal microbiota, and STI pathogens. Affordable and semi high-throughput 3D models of the cervicovaginal epithelia that are physiologically relevant by sustaining vaginal bacterial colonization, and facilitate studies of chlamydial and gonococcal infections.<\/jats:p>","DOI":"10.1093\/femspd\/ftac026","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T23:09:56Z","timestamp":1659654596000},"source":"Crossref","is-referenced-by-count":43,"title":["Three-dimensional models of the cervicovaginal epithelia to study host\u2013microbiome interactions and sexually transmitted infections"],"prefix":"10.1093","volume":"80","author":[{"given":"Vonetta L","family":"Edwards","sequence":"first","affiliation":[{"name":"Institute for Genome Sciences, University of Maryland School of Medicine , Baltimore, MD, United States"},{"name":"Department of Microbiology and Immunology, University of Maryland School of Medicine , Baltimore, MD, United 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