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Here, we studied the role of HIF1\u03b1 in myeloid cells during pneumonia caused by the major causative pathogen, Streptococcus pneumoniae (Spneu). Mice deficient for HIF1\u03b1 in myeloid cells (LysMcreHif1\u03b1fl\/fl) were generated to study the in vitro responsiveness of bone marrow-derived macrophages (BMDMs) and alveolar macrophages (AMs) to the Gram-positive bacterial wall component lipoteichoic acid (LTA) and heat-killed Spneu, and the in vivo host response after infection with Spneu via the airways. Both BMDMs and AMs released more lactate upon stimulation with LTA or Spneu, indicative of enhanced glycolysis; HIF1\u03b1-deficiency in these cells was associated with diminished lactate release. In BMDMs, HIF1\u03b1-deficiency resulted in reduced secretion of tumor necrosis factor (TNF)\u03b1 and interleukin (IL)-6 upon activation with Spneu but not LTA, while HIF1\u03b1-deficient AMs secreted less TNF\u03b1 and IL-6 in response to LTA, and TNF\u03b1 after Spneu stimulation. However, no difference was found in the host response of LysMcreHif1\u03b1fl\/fl mice after Spneu infection as compared to controls. Similar in vivo findings were obtained in neutrophil (Mrp8creHif1\u03b1fl\/fl) HIF1\u03b1-deficient mice. These data suggest that myeloid HIF1\u03b1 is dispensable for the host defense during pneumococcal pneumonia.<\/jats:p>","DOI":"10.1093\/femspd\/ftac047","type":"journal-article","created":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T00:48:56Z","timestamp":1671497336000},"source":"Crossref","is-referenced-by-count":3,"title":["Role of Hypoxia-inducible factor 1\u03b1 in host defense during pneumococcal pneumonia"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9091-7506","authenticated-orcid":false,"given":"Liza","family":"Pereverzeva","sequence":"first","affiliation":[{"name":"Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, Location Academic Medical Center, University of Amsterdam , 1105 AZ Amsterdam, the Netherlands"},{"name":"Amsterdam Infection & Immunity Institute, Amsterdam University Medical Centers, Location Academic Medical Center, University of Amsterdam , 1105 AZ Amsterdam, the 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