{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T17:22:07Z","timestamp":1780680127478,"version":"3.54.1"},"reference-count":35,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Post-doctoral funding for staying in Chongqing","award":["55012"],"award-info":[{"award-number":["55012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enterovirus 71 (EV71) can cause severe hand-foot-and-mouth disease with neurological complications. It has evolved multiple mechanisms to compromise the host type I interferon (IFN-I) response. In neuronal cells, EV71-mediated IFN-I antagonism may be associated with neural precursor cell-expressed developmentally downregulated 4-like (Nedd4L), the E3 ubiquitin ligase that can interact with alphaB-crystallin (CRYAB) in the regulation of Nav1.5 stability. Here, we investigated the effect of CRYAB stability on IFN-\u03b2 promoter activity in neuronal SH-SY5Y cells infected with EV71, and its relations to Nedd4\u00a0L and extracellular signal-regulated kinases (ERK). Results showed that EV71 infection significantly caused CRYAB degradation via the Nedd4L-proteasome pathway, which required ERK-mediated phosphorylation of Serine 45 in CRYAB. Subsequently, it was observed that siRNA- or EV71-mediated CRYAB reduction limited Poly(dAT)-activated IFN-\u03b2 promoter, and CRYAB stabilisation by U0126-mediated inhibition of ERK activation remarkably enhanced the activity of IFN-\u03b2 promoter upon EV71 challenge. Collectively, we elucidate a novel mechanism by which ERK activation contributes to EV71 immune escape via CRYAB\/IFN-\u03b2 axis in SH-SY5Y cells, indicating that perturbing ERK activation is desirable for anti-EV71 therapy.<\/jats:p>","DOI":"10.1093\/femspd\/ftad011","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T14:20:45Z","timestamp":1685542845000},"source":"Crossref","is-referenced-by-count":6,"title":["ERK inhibition aids IFN-\u03b2 promoter activation during EV71 infection by blocking CRYAB degradation in SH-SY5Y cells"],"prefix":"10.1093","volume":"81","author":[{"given":"Dengming","family":"Chen","sequence":"first","affiliation":[{"name":"Emergency and Critical Care Medicine Center, the Third Affiliated Hospital of Chongqing Medical University , Chongqing 401120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital , Chongqing 400044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyu","family":"Tan","sequence":"additional","affiliation":[{"name":"Physical Examination Center, Chongqing University Cancer Hospital , Chongqing 400044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Chongqing University Three Gorges Hospital, School of Medicine, Chong University , Chongqing 400030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6540-8726","authenticated-orcid":false,"given":"Bangtao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Chongqing University Three Gorges Hospital, School of Medicine, Chong University , Chongqing 400030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"2023070516114557500_bib1","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/nri3495","article-title":"Mitogen-activated protein kinases in innate immunity","volume":"13","author":"Arthur","year":"2013","journal-title":"Nat Rev Immunol"},{"key":"2023070516114557500_bib2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.bbagen.2015.09.017","article-title":"Phosphorylation of \u03b1B-crystallin: role in stress, aging and patho-physiological conditions","volume":"1860","author":"Bakthisaran","year":"2016","journal-title":"Biochim Biophys Acta"},{"key":"2023070516114557500_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biologicals.2022.08.007","article-title":"Recent progress and advances towards developing enterovirus 71 vaccines for effective protection against human hand, foot and 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