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The mechanisms involved in Leishmania dissemination to different areas of the skin and the internal organs remain poorly understood. Evidence shows that Very Late Antigen-4 (VLA-4)-dependent phagocyte adhesion is impaired by Leishmania infection, which may be related to the mechanisms of parasite dissemination. We investigated factors potentially associated with decreased VLA-4-mediated adhesion in Leishmania-infected macrophages, including lipid raft-mediated VLA-4 mobilization along the cellular membrane, integrin cluster formation at the cell base (adhesion site), and focal adhesion complex assembly. Phagocytes treated with Methyl-\u03b2-Cyclodextrin (M\u03b2CD) demonstrated reduced adhesion, similarly to Leishmania amazonensis-infected J774 cells. Infected and M\u03b2CD-treated macrophages presented decreased VLA-4 mobilization to the adhesion plane, as well as reduced integrin clustering. Leishmania amazonensis-infected cells exhibited talin depletion, as well as a decreased mobilization of adhesion complex proteins, such as talin and viculin, which were associated with lower VLA-4 concentrations at the adhesion site and limited cell-spreading. Our results suggest that Leishmania infection may modulate the firm adhesion phase of the cell-spreading process, which could contribute to the bloodstream dissemination of infected cells.<\/jats:p>","DOI":"10.1093\/femspd\/ftad013","type":"journal-article","created":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T06:17:34Z","timestamp":1688451454000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Leishmania amazonensis<\/i> infection impairs VLA-4 clustering and adhesion complex assembly at the adhesion site of J774 cells"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8177-4301","authenticated-orcid":false,"given":"Reginaldo","family":"Brito","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Gon\u00e7alo Moniz, Laborat\u00f3rio de Patologia Estrutural e Molecular (LAPEM) . Rua Waldemar Falc\u00e3o 121, Candeal, CEP 40296-710 , Salvador, BA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erina Masayo","family":"Hassegawa","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Gon\u00e7alo Moniz, Laborat\u00f3rio de Patologia Estrutural e Molecular (LAPEM) . Rua Waldemar Falc\u00e3o 121, Candeal, CEP 40296-710 , Salvador, BA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Camardelli","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Gon\u00e7alo Moniz, Laborat\u00f3rio de Patologia Estrutural e Molecular (LAPEM) . Rua Waldemar Falc\u00e3o 121, Candeal, CEP 40296-710 , Salvador, BA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalene","family":"Elp\u00eddio","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Gon\u00e7alo Moniz, Laborat\u00f3rio de Patologia Estrutural e Molecular (LAPEM) . Rua Waldemar Falc\u00e3o 121, Candeal, CEP 40296-710 , Salvador, BA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana","family":"de\u00a0Menezes","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Gon\u00e7alo Moniz, Laborat\u00f3rio de Intera\u00e7\u00e3o Parasito-Hospedeiro e Epidemiologia (LAIPHE) . Rua Waldemar Falc\u00e3o 121, Candeal, CEP 40296-710 , Salvador, BA, Brazil"},{"name":"Universidade Federal da Bahia, Faculdade de Medicina da Bahia. Avenida Reitor Miguel Calmon s\/n, Vale do Canela , CEP 40110-100, Salvador, BA , Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio Pereira","family":"Figueira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Gon\u00e7alo Moniz, Laborat\u00f3rio de Patologia Estrutural e Molecular (LAPEM) . Rua Waldemar Falc\u00e3o 121, Candeal, CEP 40296-710 , Salvador, BA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Washington L C","family":"dos-Santos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Gon\u00e7alo Moniz, Laborat\u00f3rio de Patologia Estrutural e Molecular (LAPEM) . Rua Waldemar Falc\u00e3o 121, Candeal, CEP 40296-710 , Salvador, BA, Brazil"},{"name":"Universidade Federal da Bahia, Faculdade de Medicina da Bahia. 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