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Unlike typical Gram-negative organisms, however, B. burgdorferi lacks lipopolysaccharide (LPS). Using computational genome analyses and structural modeling, we identified a transport system containing six proteins in B. burgdorferi that are all orthologs to proteins found in the lipopolysaccharide transport (LPT) system that links the inner and outer membranes of Gram-negative organisms and is responsible for placing LPS on the surface of these organisms. While B. burgdorferi does not contain LPS, it does encode over 100 different surface-exposed lipoproteins and several major glycolipids, which like LPS are also highly amphiphilic molecules, though no system to transport these molecules to the borrelial surface is known. Accordingly, experiments supplemented by molecular modeling were undertaken to determine whether the orthologous LPT system identified in B. burgdorferi could transport lipoproteins and\/or glycolipids to the borrelial outer membrane. Our combined observations strongly suggest that the LPT transport system does not transport lipoproteins to the surface. Molecular dynamic modeling, however, suggests that the borrelial LPT system could transport borrelial glycolipids to the outer membrane.<\/jats:p>","DOI":"10.1093\/femspd\/ftad014","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T01:48:51Z","timestamp":1688089731000},"source":"Crossref","is-referenced-by-count":6,"title":["Identification of a novel transport system in<i>Borrelia burgdorferi<\/i>that links the inner and outer membranes"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3712-664X","authenticated-orcid":false,"given":"Hannah G","family":"Bowen","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center , 940 Stanton L. Young Blvd., BMSB 1053 Oklahoma City, OK 73104 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melisha R","family":"Kenedy","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center , 940 Stanton L. Young Blvd., BMSB 1053 Oklahoma City, OK 73104 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David K","family":"Johnson","sequence":"additional","affiliation":[{"name":"Shenkel Structural Biology Center, Molecular Graphics and Modeling Laboratory and the Computational Biology Core, University of Kansas , 2034 Becker Drive Lawrence, Kansas 66047 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Alexander D","family":"MacKerell","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland , Baltimore 20 North Pine Street Baltimore, Maryland 21201 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darrin R","family":"Akins","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center , 940 Stanton L. 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