{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:00:46Z","timestamp":1753876846131,"version":"3.41.2"},"reference-count":20,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270203"],"award-info":[{"award-number":["32270203"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chlamydia trachomatis is an obligate intracellular bacterium that causes blinding trachoma and sexually transmitted disease. The chlamydial plasmid is a critical virulence factor in the pathogenesis of these diseases. Plasmid gene protein 4 (Pgp4) plays a major role in chlamydial virulence by regulating the expression of both chromosomal genes and Pgp3. Despite the importance of Pgp4 in mediating lytic exit from host cells the pathogenic mechanism by which it functions is unknown. CT084 is a highly conserved chromosomal gene with homology to phospholipase D. We showed CT084 expression is regulated by Pgp4 and expressed late in the chlamydial developmental cycle. To investigate the function of CT084 in chlamydial lytic exit from infected cells, we made a CT084 null strain (ct084::bla) by using Targetron. The ct084::bla strain grew normally in vitro compared to wild-type strain; however, the strain did not lyse infected cells and produced significantly less and smaller plaques. Collectively, our finding shows Pgp4-regulated CT084-mediated chlamydia lytic exit from infected host cells.<\/jats:p>","DOI":"10.1093\/femspd\/ftad026","type":"journal-article","created":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T11:26:15Z","timestamp":1696677975000},"source":"Crossref","is-referenced-by-count":1,"title":["TargeTron inactivation of plasmid-regulated <i>Chlamydia trachomatis<\/i> CT084 results in a nonlytic phenotype"],"prefix":"10.1093","volume":"81","author":[{"given":"Una","family":"Karanovic","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Disease, National Institutes of Health , Bethesda, Maryland, 208952 , United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Lei","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Disease, National Institutes of Health , Bethesda, Maryland, 208952 , United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig A","family":"Martens","sequence":"additional","affiliation":[{"name":"Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases , Hamilton, Montana, 59840 , United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kent","family":"Barbian","sequence":"additional","affiliation":[{"name":"Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases , Hamilton, Montana, 59840 , United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant","family":"McClarty","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, University of Manitoba , Winnipeg, Manitoba R3T 2N2 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harlan 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