{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:58:16Z","timestamp":1753876696345,"version":"3.41.2"},"reference-count":31,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":120,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We have demonstrated previously that TNF-\u03b1-producing CD8+ T cells mediate chlamydial pathogenesis, likely in an antigen (Ag)-specific fashion. Here we hypothesize that inhibition of Ag-specific CD8+ T cell response after immunization and\/or challenge would correlate with protection against oviduct pathology induced by a protective vaccine regimen. Intranasal (i.n.) live chlamydial elementary body (EB), intramuscular (i.m.) live EB, or i.n. irrelevant antigen, bovine serum albumin (BSA), immunized animals induced near-total protection, 50% protection, or no protection, respectively against oviduct pathology following i.vag. C. muridarum challenge. In these models, we evaluated Ag-specific CD8+ T cell cytokine response at various time-periods after immunization or challenge. The results show protective efficacy of vaccine regimens correlated with reduction of Ag-specific CD8+ T cell TNF-\u03b1 responses following i.vag. chlamydial challenge, not after immunization. Depletion of CD4+ T cells abrogated, whereas adoptive transfer of Ag-specific CD4+ T cells induced the significant reduction of Ag-specific CD8+ T cell TNF-\u03b1 response after chlamydial challenge. In conclusion, protective anti-chlamydial vaccine regimens induce Ag-specific CD4+ T cell response that mediate early inhibition of pathogenic CD8+ T cell response following challenge and may serve as a predictive biomarker of protection against Chlamydia -induced chronic pathologies.<\/jats:p>","DOI":"10.1093\/femspd\/ftae008","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T02:49:19Z","timestamp":1714445359000},"source":"Crossref","is-referenced-by-count":2,"title":["Protective anti-chlamydial vaccine regimen-induced CD4+ T cell response mediates early inhibition of pathogenic CD8+ T cell response following genital challenge"],"prefix":"10.1093","volume":"82","author":[{"given":"Ashlesh K","family":"Murthy","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Midwestern University , Glendale, AZ 85308 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,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarakarama","family":"Musunuri","sequence":"additional","affiliation":[{"name":"Arizona College of Osteopathic Medicine, Midwestern University , Glendale, AZ 85308 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srikanth","family":"Manam","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Midwestern University , Glendale, AZ 85308 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemence Z","family":"Chako","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Midwestern University , Glendale, AZ 85308 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle H","family":"Ramsey","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Midwestern University , Glendale, AZ 85308 ,","place":["USA"]},{"name":"College of Graduate 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